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Database: UniProt
Entry: Q22710
LinkDB: Q22710
Original site: Q22710 
ID   NAS26_CAEEL             Reviewed;         414 AA.
AC   Q22710;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 4.
DT   10-APR-2019, entry version 128.
DE   RecName: Full=Zinc metalloproteinase nas-26;
DE            EC=3.4.24.- {ECO:0000250|UniProtKB:A8Q2D1};
DE   AltName: Full=Nematode astacin 26;
DE   AltName: Full=Tollish protein 1;
DE   Flags: Precursor;
GN   Name=toh-1; Synonyms=nas-26; ORFNames=T24A11.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   IDENTIFICATION.
RA   Finelli A.L., Savage C., Cho S.H., Padgett R.W.;
RT   "Tollish genes in C. elegans.";
RL   (er) Worm Breeder's Gazette 14(2):46(1996).
RN   [3]
RP   IDENTIFICATION, AND NOMENCLATURE.
RX   PubMed=14653817; DOI=10.1046/j.1432-1033.2003.03891.x;
RA   Moehrlen F., Hutter H., Zwilling R.;
RT   "The astacin protein family in Caenorhabditis elegans.";
RL   Eur. J. Biochem. 270:4909-4920(2003).
CC   -!- FUNCTION: Metalloprotease. {ECO:0000250|UniProtKB:A8Q2D1}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU01211};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000255|PROSITE-
CC       ProRule:PRU01211};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
DR   EMBL; Z49072; CAA88882.4; -; Genomic_DNA.
DR   PIR; T25213; T25213.
DR   RefSeq; NP_497769.3; NM_065368.5.
DR   UniGene; Cel.22628; -.
DR   ProteinModelPortal; Q22710; -.
DR   SMR; Q22710; -.
DR   STRING; 6239.T24A11.3; -.
DR   MEROPS; M12.A17; -.
DR   EPD; Q22710; -.
DR   PaxDb; Q22710; -.
DR   PeptideAtlas; Q22710; -.
DR   EnsemblMetazoa; T24A11.3; T24A11.3; WBGene00006591.
DR   GeneID; 175491; -.
DR   KEGG; cel:CELE_T24A11.3; -.
DR   UCSC; T24A11.3; c. elegans.
DR   CTD; 175491; -.
DR   WormBase; T24A11.3; CE35909; WBGene00006591; toh-1.
DR   eggNOG; KOG3714; Eukaryota.
DR   eggNOG; ENOG410ZPX7; LUCA.
DR   HOGENOM; HOG000018812; -.
DR   InParanoid; Q22710; -.
DR   KO; K08076; -.
DR   OMA; CYWRISV; -.
DR   OrthoDB; 681837at2759; -.
DR   PhylomeDB; Q22710; -.
DR   PRO; PR:Q22710; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00006591; Expressed in 4 organ(s), highest expression level in multi-cellular organism.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0018996; P:molting cycle, collagen and cuticulin-based cuticle; IEA:InterPro.
DR   CDD; cd00041; CUB; 1.
DR   CDD; cd04280; ZnMc_astacin_like; 1.
DR   Gene3D; 2.60.120.290; -; 1.
DR   Gene3D; 3.40.390.10; -; 1.
DR   InterPro; IPR034035; Astacin-like_dom.
DR   InterPro; IPR000859; CUB_dom.
DR   InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR   InterPro; IPR017050; Metallopeptidase_nem.
DR   InterPro; IPR001506; Peptidase_M12A.
DR   InterPro; IPR006026; Peptidase_Metallo.
DR   InterPro; IPR035914; Sperma_CUB_dom_sf.
DR   Pfam; PF01400; Astacin; 1.
DR   Pfam; PF00431; CUB; 1.
DR   PIRSF; PIRSF036365; Astacin_nematoda; 1.
DR   PRINTS; PR00480; ASTACIN.
DR   SMART; SM00042; CUB; 1.
DR   SMART; SM00235; ZnMc; 1.
DR   SUPFAM; SSF49854; SSF49854; 1.
DR   PROSITE; PS51864; ASTACIN; 1.
DR   PROSITE; PS01180; CUB; 1.
DR   PROSITE; PS00022; EGF_1; 1.
PE   3: Inferred from homology;
KW   Cleavage on pair of basic residues; Complete proteome; Disulfide bond;
KW   EGF-like domain; Glycoprotein; Hydrolase; Metal-binding;
KW   Metalloprotease; Protease; Reference proteome; Secreted; Signal; Zinc;
KW   Zymogen.
FT   SIGNAL        1     20       {ECO:0000255}.
FT   PROPEP       21     61       {ECO:0000250|UniProtKB:P13497}.
FT                                /FTId=PRO_0000442673.
FT   CHAIN        62    414       Zinc metalloproteinase nas-26.
FT                                /FTId=PRO_0000028930.
FT   DOMAIN       62    264       Peptidase M12A. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01211}.
FT   DOMAIN      251    307       EGF-like.
FT   DOMAIN      308    414       CUB. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00059}.
FT   ACT_SITE    155    155       {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   METAL       154    154       Zinc; via tele nitrogen; catalytic.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   METAL       158    158       Zinc; via tele nitrogen; catalytic.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   METAL       164    164       Zinc; via tele nitrogen; catalytic.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   CARBOHYD     24     24       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   DISULFID    103    263       {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   DISULFID    126    146       {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   DISULFID    267    286       {ECO:0000255|PROSITE-ProRule:PRU00059}.
FT   DISULFID    289    300       {ECO:0000255|PROSITE-ProRule:PRU00059}.
FT   DISULFID    308    331       {ECO:0000255|PROSITE-ProRule:PRU00059}.
FT   DISULFID    358    378       {ECO:0000255|PROSITE-ProRule:PRU00059}.
SQ   SEQUENCE   414 AA;  47140 MW;  B8B381B421239D1B CRC64;
     MTSSLVLILA PLALVAIGEA AFGNSSKIFE IPGLEVMASD KYPHFTTIET VSRTKVHRHR
     REVIAGQIYD WNSYEIPFQI WGGDYNFQSL IRRGIRMWED STCLRFKENQ QSRDAIRYVL
     EKGDSCFTEY IGRNGGHQDI IIGSECAEEY VVAHETGHAL GFWHTHQRPD RDRHISINWK
     NVMEEATASF MPFRSMLQAF GIRQVSPRRV PYDYGSLMHY HAVAHAVKVS DFTIVPKELK
     YVTTMGTEKM AFLDAKVIND IYCPNACQGR NHLNCLAGGY PDPNNCNVCR CPEGLGGPDC
     GRLQPSPCGG EIHASDQWQT LSSPSGRDVH CYWRISVPEG SRVRFRLSDG EFPCSYGCQS
     YVEIKHKLDV RLTGFRSCCY RPKEDTVSES NQIFVIYHPN GRTARFSLRF RRQA
//
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