ID Q250T7_DESHY Unreviewed; 825 AA.
AC Q250T7;
DT 18-APR-2006, integrated into UniProtKB/TrEMBL.
DT 18-APR-2006, sequence version 1.
DT 27-MAR-2024, entry version 92.
DE SubName: Full=Pyruvate-formate lyase {ECO:0000313|EMBL:BAE82205.1};
DE EC=2.3.1.54 {ECO:0000313|EMBL:BAE82205.1};
GN OrderedLocusNames=DSY0416 {ECO:0000313|EMBL:BAE82205.1};
OS Desulfitobacterium hafniense (strain Y51).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC Desulfitobacterium.
OX NCBI_TaxID=138119 {ECO:0000313|EMBL:BAE82205.1, ECO:0000313|Proteomes:UP000001946};
RN [1] {ECO:0000313|EMBL:BAE82205.1, ECO:0000313|Proteomes:UP000001946}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Y51 {ECO:0000313|EMBL:BAE82205.1,
RC ECO:0000313|Proteomes:UP000001946};
RX PubMed=16513756; DOI=10.1128/JB.188.6.2262-2274.2006;
RA Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K.,
RA Inatomi K., Furukawa K., Inui M., Yukawa H.;
RT "Complete genome sequence of the dehalorespiring bacterium
RT Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides
RT ethenogenes 195.";
RL J. Bacteriol. 188:2262-2274(2006).
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DR EMBL; AP008230; BAE82205.1; -; Genomic_DNA.
DR RefSeq; WP_011459062.1; NC_007907.1.
DR AlphaFoldDB; Q250T7; -.
DR SMR; Q250T7; -.
DR STRING; 138119.DSY0416; -.
DR KEGG; dsy:DSY0416; -.
DR eggNOG; COG1882; Bacteria.
DR HOGENOM; CLU_009096_0_1_9; -.
DR Proteomes; UP000001946; Chromosome.
DR GO; GO:0008861; F:formate C-acetyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR CDD; cd01677; PFL2_DhaB_BssA; 1.
DR Gene3D; 3.20.70.20; -; 1.
DR InterPro; IPR019777; Form_AcTrfase_GR_CS.
DR InterPro; IPR001150; Gly_radical.
DR InterPro; IPR004184; PFL_dom.
DR PANTHER; PTHR43641:SF2; DEHYDRATASE YBIW-RELATED; 1.
DR PANTHER; PTHR43641; FORMATE ACETYLTRANSFERASE 3-RELATED; 1.
DR Pfam; PF01228; Gly_radical; 1.
DR Pfam; PF02901; PFL-like; 1.
DR SUPFAM; SSF51998; PFL-like glycyl radical enzymes; 1.
DR PROSITE; PS00850; GLY_RADICAL_1; 1.
DR PROSITE; PS51149; GLY_RADICAL_2; 1.
DR PROSITE; PS51554; PFL; 1.
PE 4: Predicted;
KW Acyltransferase {ECO:0000313|EMBL:BAE82205.1};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:BAE82205.1};
KW Organic radical {ECO:0000256|ARBA:ARBA00022818, ECO:0000256|PROSITE-
KW ProRule:PRU00493}; Pyruvate {ECO:0000313|EMBL:BAE82205.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001946};
KW Transferase {ECO:0000313|EMBL:BAE82205.1}.
FT DOMAIN 27..695
FT /note="PFL"
FT /evidence="ECO:0000259|PROSITE:PS51554"
FT DOMAIN 702..825
FT /note="Glycine radical"
FT /evidence="ECO:0000259|PROSITE:PS51149"
FT MOD_RES 800
FT /note="Glycine radical"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00493"
SQ SEQUENCE 825 AA; 93581 MW; 9382F3820F1C1D05 CRC64;
MSTCCLSPHE ERIIDPQKGK VNYEGRERVF KILESFQDLR PKIDVERAKY FTESFKATEG
QPLQPRWAKA LMHIAQNITV YIDDQQLIVG RAGAQGRYGI VFPELDGDFL GLAIEQLPQR
VESPFNIDPD DAQIIINDVA PYWKGKTFHE ELAKALPEDT LKVTYDPADP LASRFIVNET
ASFRSSIQWV HDYEKVLKRG FKGIKEEAQA KLDQLDPLSP ADNMEKRPFL EAVIITCDAI
VVWANRHAHL AAQMAKLEKD PQRKQELSDI AERCAWVPEH PARNFREAVQ SQWFVQMFSR
IEQKTGTIIS NGRMDQYFYP YYQKDVAEGI LTPQQAIELL ECMWVGMAQF IDLYLSPTGG
AFNEGYAHWE AVTVGGQTPD GVDATNELTY LFLESKQKFP LNYPDLAARI HSRSPERYLY
EVAETVKEGT GFPKLINDEE VVPLLLSKGA KFAEALDYAV SGCAECRMPN RDTYTSGNPY
INFAAAIEMT LYNGRMLKYG DEQLGLETGD PTQFETWDEF WKAYLAQQTN FLKHAFIQQH
IIIRLRAQHF ASPLGSLMHD LCMENCLDLH QPVIKGGIDL GYFELIGYGT VVDSLAAIKK
LVYEEKKLTM AELLEAVRNN FEGHEVIREM VMHTPKYGNN DPYSDVIAKE VDRTAVEFTK
KYSRELGVHL DLRLVPFTSH VPFGKVVSAT PNGRKAWMPL ADGSSASHGA DVNGPTAVLL
SNFFSKNYGY RNRAARLLNI KLSPSCVAGE EGSEKLVSFI RTWCDLKLWH IQFNIVNRET
LLAAKQDPDK YRGLIVRVAG YSAYFVDLSS DLQDDIIART EHEML
//
