GenomeNet

Database: UniProt
Entry: Q27564
LinkDB: Q27564
Original site: Q27564 
ID   KITH_DICDI              Reviewed;         227 AA.
AC   Q27564; Q54HT8;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   05-DEC-2018, entry version 105.
DE   RecName: Full=Thymidine kinase 1;
DE            Short=TK1;
DE            EC=2.7.1.21;
DE   AltName: Full=Calmodulin-binding protein ThyB;
GN   Name=thyB {ECO:0000312|EMBL:AAB03673.1}; ORFNames=DDB_G0289179;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyostelids; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAM44288.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH CALMODULIN.
RX   PubMed=15883042; DOI=10.1016/j.bbrc.2005.04.074;
RA   O'Day D.H., Chatterjee-Chakraborty M., Wagler S., Myre M.A.;
RT   "Isolation and characterization of Dictyostelium thymidine kinase 1 as
RT   a calmodulin-binding protein.";
RL   Biochem. Biophys. Res. Commun. 331:1494-1502(2005).
RN   [2] {ECO:0000305, ECO:0000312|EMBL:AAO64434.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL
RP   PROPERTIES.
RC   STRAIN=AX4 {ECO:0000269|PubMed:17448496};
RX   PubMed=17448496; DOI=10.1016/j.jmb.2007.03.053;
RA   Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.;
RT   "Dictyostelium discoideum salvages purine deoxyribonucleosides by
RT   highly specific bacterial-like deoxyribonucleoside kinases.";
RL   J. Mol. Biol. 369:653-664(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AX4;
RX   PubMed=8643615; DOI=10.1073/pnas.93.11.5562;
RA   Kuspa A., Loomis W.F.;
RT   "Ordered yeast artificial chromosome clones representing the
RT   Dictyostelium discoideum genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996).
RN   [4] {ECO:0000305, ECO:0000312|EMBL:EAL62823.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4 {ECO:0000312|EMBL:EAL62823.1};
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [5] {ECO:0000305}
RP   DEVELOPMENTAL STAGE.
RX   PubMed=11923193;
RA   Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M.,
RA   Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I.,
RA   Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y.,
RA   Shaulsky G.;
RT   "A transcriptional profile of multicellular development in
RT   Dictyostelium discoideum.";
RL   Development 129:1543-1552(2002).
RN   [6] {ECO:0000305}
RP   DEVELOPMENTAL STAGE.
RX   PubMed=12912885; DOI=10.1128/EC.2.4.664-670.2003;
RA   Iranfar N., Fuller D., Loomis W.F.;
RT   "Genome-wide expression analyses of gene regulation during early
RT   development of Dictyostelium discoideum.";
RL   Eukaryot. Cell 2:664-670(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+);
CC         Xref=Rhea:RHEA:19129, ChEBI:CHEBI:15378, ChEBI:CHEBI:17748,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:63528, ChEBI:CHEBI:456216;
CC         EC=2.7.1.21; Evidence={ECO:0000269|PubMed:17448496};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=5.1 uM for thymidine {ECO:0000269|PubMed:17448496};
CC   -!- SUBUNIT: Interacts with calmodulin in the presence of Ca(2+).
CC       {ECO:0000269|PubMed:15883042}.
CC   -!- DEVELOPMENTAL STAGE: Expression levels decrease throughout
CC       development. {ECO:0000269|PubMed:11923193,
CC       ECO:0000269|PubMed:12912885}.
CC   -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000255}.
DR   EMBL; AY192984; AAO64434.1; -; mRNA.
DR   EMBL; AF510846; AAM44288.1; -; mRNA.
DR   EMBL; U61990; AAB03673.1; -; mRNA.
DR   EMBL; AAFI02000131; EAL62823.1; -; Genomic_DNA.
DR   RefSeq; XP_636351.1; XM_631259.1.
DR   ProteinModelPortal; Q27564; -.
DR   SMR; Q27564; -.
DR   MINT; Q27564; -.
DR   STRING; 44689.DDB0191436; -.
DR   PaxDb; Q27564; -.
DR   EnsemblProtists; EAL62823; EAL62823; DDB_G0289179.
DR   GeneID; 8627024; -.
DR   KEGG; ddi:DDB_G0289179; -.
DR   dictyBase; DDB_G0289179; thyB.
DR   eggNOG; KOG3125; Eukaryota.
DR   eggNOG; COG1435; LUCA.
DR   InParanoid; Q27564; -.
DR   KO; K00857; -.
DR   OMA; CAGLDQD; -.
DR   PhylomeDB; Q27564; -.
DR   BRENDA; 2.7.1.21; 1939.
DR   Reactome; R-DDI-73614; Pyrimidine salvage.
DR   SABIO-RK; Q27564; -.
DR   PRO; PR:Q27564; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   Proteomes; UP000002195; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IC:dictyBase.
DR   GO; GO:0005516; F:calmodulin binding; IPI:dictyBase.
DR   GO; GO:0004683; F:calmodulin-dependent protein kinase activity; IDA:dictyBase.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:dictyBase.
DR   GO; GO:0004797; F:thymidine kinase activity; IDA:dictyBase.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006259; P:DNA metabolic process; IBA:GO_Central.
DR   GO; GO:0006139; P:nucleobase-containing compound metabolic process; IDA:dictyBase.
DR   GO; GO:0046104; P:thymidine metabolic process; IBA:GO_Central.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001267; Thymidine_kinase.
DR   InterPro; IPR020633; Thymidine_kinase_CS.
DR   PANTHER; PTHR11441; PTHR11441; 1.
DR   Pfam; PF00265; TK; 1.
DR   PIRSF; PIRSF035805; TK_cell; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00603; TK_CELLULAR_TYPE; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Calmodulin-binding; Complete proteome; DNA synthesis;
KW   Kinase; Metal-binding; Nucleotide-binding; Reference proteome;
KW   Transferase; Zinc.
FT   CHAIN         1    227       Thymidine kinase 1.
FT                                /FTId=PRO_0000293627.
FT   NP_BIND      15     22       ATP. {ECO:0000255, ECO:0000305}.
FT   NP_BIND      47     49       ATP. {ECO:0000250}.
FT   NP_BIND      91     94       ATP. {ECO:0000250}.
FT   REGION      166    170       Substrate binding. {ECO:0000250}.
FT   ACT_SITE     92     92       Proton acceptor. {ECO:0000255}.
FT   METAL       147    147       Zinc. {ECO:0000250}.
FT   METAL       150    150       Zinc. {ECO:0000250}.
FT   METAL       179    179       Zinc. {ECO:0000250}.
FT   METAL       182    182       Zinc. {ECO:0000250}.
FT   BINDING     122    122       Substrate; via amide nitrogen.
FT                                {ECO:0000250}.
FT   BINDING     175    175       Substrate. {ECO:0000250}.
SQ   SEQUENCE   227 AA;  25494 MW;  CF96E644406C0772 CRC64;
     MIVTQIAGKI QVIFGPMFSG KTTELIRRIK RFNFANKKCL LIKYSKDTRY NDNIDKSFLV
     THDKQNYQAF PCSILEDVKE QAQNYDVIGI DEGQFFPDVV QFSEDLANQG KTVIIAALDG
     TFQRKPFQSV IDLVSKAEYI TKLTAVCMVC YNEAAFSKRI VESDDIELIG GIDKYISVCR
     GCYNSDQNEG NSTKPSKTAR HSHSQSAPSV APLAVNINPD DHLNNDY
//
DBGET integrated database retrieval system