GenomeNet

Database: UniProt
Entry: Q2GV49
LinkDB: Q2GV49
Original site: Q2GV49 
ID   SPB4_CHAGB              Reviewed;         646 AA.
AC   Q2GV49;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   18-SEP-2019, entry version 70.
DE   RecName: Full=ATP-dependent rRNA helicase SPB4 {ECO:0000305};
DE            EC=3.6.4.13 {ECO:0000250|UniProtKB:P25808};
GN   Name=SPB4 {ECO:0000250|UniProtKB:P25808}; ORFNames=CHGG_08155;
OS   Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC
OS   6347 / NRRL 1970) (Soil fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Sordariomycetes; Sordariomycetidae; Sordariales; Chaetomiaceae;
OC   Chaetomium.
OX   NCBI_TaxID=306901;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970;
RX   PubMed=25720678; DOI=10.1128/genomea.00021-15;
RA   Cuomo C.A., Untereiner W.A., Ma L.-J., Grabherr M., Birren B.W.;
RT   "Draft genome sequence of the cellulolytic fungus Chaetomium
RT   globosum.";
RL   Genome Announc. 3:E0002115-E0002115(2015).
CC   -!- FUNCTION: ATP-binding RNA helicase involved in the biogenesis of
CC       60S ribosomal subunits. Binds 90S pre-ribosomal particles and
CC       dissociates from pre-60S ribosomal particles after processing of
CC       27SB pre-rRNA. Required for the normal formation of 18S rRNA
CC       through the processing of pre-rRNAs at sites A0, A1 and A2, and
CC       the normal formation of 25S and 5.8S rRNAs through the processing
CC       of pre-rRNAs at sites C1 and C2. {ECO:0000250|UniProtKB:P25808}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000250|UniProtKB:P25808};
CC   -!- SUBUNIT: Component of pre-60S ribosomal complexes.
CC       {ECO:0000250|UniProtKB:P25808}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:P25808}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX55/SPB4
CC       subfamily. {ECO:0000305}.
DR   EMBL; CH408033; EAQ86902.1; -; Genomic_DNA.
DR   RefSeq; XP_001225811.1; XM_001225810.1.
DR   SMR; Q2GV49; -.
DR   STRING; 38033.XP_001225811.1; -.
DR   PRIDE; Q2GV49; -.
DR   EnsemblFungi; EAQ86902; EAQ86902; CHGG_08155.
DR   GeneID; 4393902; -.
DR   eggNOG; KOG0345; Eukaryota.
DR   eggNOG; ENOG410XNT7; LUCA.
DR   InParanoid; Q2GV49; -.
DR   OrthoDB; 973872at2759; -.
DR   Proteomes; UP000001056; Unassembled WGS sequence.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR025313; DUF4217.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF13959; DUF4217; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM01178; DUF4217; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Coiled coil; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA processing.
FT   CHAIN         1    646       ATP-dependent rRNA helicase SPB4.
FT                                /FTId=PRO_0000256048.
FT   DOMAIN       46    247       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      284    434       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      59     66       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   COILED      572    623       {ECO:0000255}.
FT   MOTIF        15     43       Q motif. {ECO:0000305}.
FT   MOTIF       195    198       DEAD box. {ECO:0000305}.
SQ   SEQUENCE   646 AA;  71577 MW;  969A97452C531D85 CRC64;
     MPPEQVRARK TPRSWGALTP SLAPWILDYL SSMGFEQPTP VQKSCFDIFR GNKDVVVEAV
     TGSGKTLAFL IPVVERLLRS EDPAKRHHVQ AIIISPTREL ASQIYNVLVS FLKFHAPSAH
     LLPHAKGDEK RPATTEPVVV PQLLVGGTTK AAEDLSTFLR LSPNILIGTP GRLAELLSTP
     YVKAPSSSFE VLVMDEADRL LDLGFSQELT RILGYLPKQR RTGLFSASLS EAVERLITVG
     LLYPHKITVR VKSLRDGGIV QERKTPMSLQ MSYIVTPASQ KIPALCQLLE RLEPRPSRSI
     VFFSTCFAVK YFARVLHGIL PPGFSIVSLH GKLEPQVREK NFERFVNAAS PTVLLTTDIA
     ARGLDIPQVD LVVQHDPPTD TKVFIHRCGR AGRAGRRGLA VVMLQPGREE GYVQLLDVRQ
     TPISPLAKPP VSVTAADADL ASSRIRAQAR ADRDIYQLAQ RAFVSWARSY IEHQATSIFR
     VADLDWADLA QGYGLLELPK MPEVRGLDRS LGLAIDTESI PFRDGAREKK RLAELEQWKK
     EKAAREAQRA SGGGGDATDP LKRKKNEAWS GKHEHEDVKA ARREKKRRKR EAQRLGDMTE
     PEREEQRKLD EMIAEVRRRN AEAPTPAAQA AGSGGGGGDE FEGFDD
//
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