GenomeNet

Database: UniProt
Entry: Q2UCU3
LinkDB: Q2UCU3
Original site: Q2UCU3 
ID   BGALA_ASPOR             Reviewed;        1005 AA.
AC   Q2UCU3;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   10-APR-2019, entry version 82.
DE   RecName: Full=Probable beta-galactosidase A;
DE            EC=3.2.1.23;
DE   AltName: Full=Lactase A;
DE   Flags: Precursor;
GN   Name=lacA; ORFNames=AO090012000445;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G.,
RA   Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K.,
RA   Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W.,
RA   Galagan J.E., Nierman W.C., Yu J., Archer D.B., Bennett J.W.,
RA   Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H.,
RA   Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R.,
RA   Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N.,
RA   Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I.,
RA   Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y.,
RA   Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y.,
RA   Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K.,
RA   Kuhara S., Ogasawara N., Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- FUNCTION: Cleaves beta-linked terminal galactosyl residues from
CC       gangliosides, glycoproteins, and glycosaminoglycans.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose
CC         residues in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000305}.
DR   EMBL; AP007161; BAE60622.1; -; Genomic_DNA.
DR   RefSeq; XP_001727461.1; XM_001727409.1.
DR   PDB; 4IUG; X-ray; 2.60 A; A=1-1005.
DR   PDBsum; 4IUG; -.
DR   ProteinModelPortal; Q2UCU3; -.
DR   SMR; Q2UCU3; -.
DR   Allergome; 1261; Asp o Lactase.
DR   CAZy; GH35; Glycoside Hydrolase Family 35.
DR   mycoCLAP; LAC35A_ASPOR; -.
DR   EnsemblFungi; BAE60622; BAE60622; AO090012000445.
DR   GeneID; 5987935; -.
DR   KEGG; aor:AO090012000445; -.
DR   OMA; GGEDYVD; -.
DR   Proteomes; UP000006564; Chromosome 4.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.102.20.10; -; 1.
DR   Gene3D; 2.60.120.260; -; 2.
DR   Gene3D; 2.60.390.10; -; 1.
DR   InterPro; IPR018954; Betagal_dom2.
DR   InterPro; IPR037110; Betagal_dom2_sf.
DR   InterPro; IPR025972; BetaGal_dom3.
DR   InterPro; IPR036833; BetaGal_dom3_sf.
DR   InterPro; IPR025300; BetaGal_jelly_roll_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421; PTHR23421; 1.
DR   Pfam; PF10435; BetaGal_dom2; 1.
DR   Pfam; PF13363; BetaGal_dom3; 1.
DR   Pfam; PF13364; BetaGal_dom4_5; 2.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SMART; SM01029; BetaGal_dom2; 1.
DR   SUPFAM; SSF117100; SSF117100; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Carbohydrate metabolism; Complete proteome;
KW   Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL        1     18       {ECO:0000255}.
FT   CHAIN        19   1005       Probable beta-galactosidase A.
FT                                /FTId=PRO_0000395218.
FT   COMPBIAS    175    184       Poly-Ala.
FT   ACT_SITE    200    200       Proton donor. {ECO:0000255}.
FT   ACT_SITE    298    298       Nucleophile. {ECO:0000255}.
FT   BINDING      96     96       Substrate. {ECO:0000250}.
FT   BINDING     140    140       Substrate. {ECO:0000250}.
FT   BINDING     141    141       Substrate; via amide nitrogen.
FT                                {ECO:0000250}.
FT   BINDING     142    142       Substrate. {ECO:0000250}.
FT   BINDING     199    199       Substrate. {ECO:0000250}.
FT   BINDING     260    260       Substrate. {ECO:0000250}.
FT   BINDING     364    364       Substrate. {ECO:0000250}.
FT   CARBOHYD    156    156       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    373    373       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    402    402       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    453    453       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    478    478       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    522    522       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    622    622       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    760    760       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    777    777       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    805    805       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    914    914       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   DISULFID    205    206       {ECO:0000250}.
FT   DISULFID    266    315       {ECO:0000250}.
FT   STRAND       44     48       {ECO:0000244|PDB:4IUG}.
FT   STRAND       53     55       {ECO:0000244|PDB:4IUG}.
FT   STRAND       58     60       {ECO:0000244|PDB:4IUG}.
FT   STRAND       62     67       {ECO:0000244|PDB:4IUG}.
FT   HELIX        69     71       {ECO:0000244|PDB:4IUG}.
FT   HELIX        75     77       {ECO:0000244|PDB:4IUG}.
FT   HELIX        78     87       {ECO:0000244|PDB:4IUG}.
FT   STRAND       92     96       {ECO:0000244|PDB:4IUG}.
FT   HELIX        99    102       {ECO:0000244|PDB:4IUG}.
FT   HELIX       113    115       {ECO:0000244|PDB:4IUG}.
FT   HELIX       118    127       {ECO:0000244|PDB:4IUG}.
FT   STRAND      130    137       {ECO:0000244|PDB:4IUG}.
FT   HELIX       144    147       {ECO:0000244|PDB:4IUG}.
FT   HELIX       150    154       {ECO:0000244|PDB:4IUG}.
FT   HELIX       164    169       {ECO:0000244|PDB:4IUG}.
FT   HELIX       171    183       {ECO:0000244|PDB:4IUG}.
FT   HELIX       186    188       {ECO:0000244|PDB:4IUG}.
FT   STRAND      190    200       {ECO:0000244|PDB:4IUG}.
FT   HELIX       213    224       {ECO:0000244|PDB:4IUG}.
FT   TURN        225    227       {ECO:0000244|PDB:4IUG}.
FT   STRAND      236    240       {ECO:0000244|PDB:4IUG}.
FT   STRAND      257    259       {ECO:0000244|PDB:4IUG}.
FT   HELIX       281    288       {ECO:0000244|PDB:4IUG}.
FT   STRAND      295    302       {ECO:0000244|PDB:4IUG}.
FT   HELIX       312    318       {ECO:0000244|PDB:4IUG}.
FT   HELIX       321    332       {ECO:0000244|PDB:4IUG}.
FT   TURN        333    335       {ECO:0000244|PDB:4IUG}.
FT   STRAND      337    345       {ECO:0000244|PDB:4IUG}.
FT   HELIX       350    352       {ECO:0000244|PDB:4IUG}.
FT   HELIX       377    390       {ECO:0000244|PDB:4IUG}.
FT   HELIX       393    395       {ECO:0000244|PDB:4IUG}.
FT   STRAND      404    410       {ECO:0000244|PDB:4IUG}.
FT   STRAND      412    420       {ECO:0000244|PDB:4IUG}.
FT   STRAND      422    424       {ECO:0000244|PDB:4IUG}.
FT   STRAND      426    434       {ECO:0000244|PDB:4IUG}.
FT   STRAND      440    443       {ECO:0000244|PDB:4IUG}.
FT   STRAND      445    449       {ECO:0000244|PDB:4IUG}.
FT   STRAND      452    460       {ECO:0000244|PDB:4IUG}.
FT   STRAND      463    468       {ECO:0000244|PDB:4IUG}.
FT   STRAND      471    479       {ECO:0000244|PDB:4IUG}.
FT   STRAND      482    497       {ECO:0000244|PDB:4IUG}.
FT   STRAND      500    507       {ECO:0000244|PDB:4IUG}.
FT   STRAND      512    518       {ECO:0000244|PDB:4IUG}.
FT   STRAND      524    528       {ECO:0000244|PDB:4IUG}.
FT   STRAND      534    538       {ECO:0000244|PDB:4IUG}.
FT   STRAND      541    547       {ECO:0000244|PDB:4IUG}.
FT   STRAND      553    557       {ECO:0000244|PDB:4IUG}.
FT   STRAND      560    566       {ECO:0000244|PDB:4IUG}.
FT   HELIX       567    570       {ECO:0000244|PDB:4IUG}.
FT   STRAND      580    583       {ECO:0000244|PDB:4IUG}.
FT   HELIX       590    594       {ECO:0000244|PDB:4IUG}.
FT   STRAND      598    600       {ECO:0000244|PDB:4IUG}.
FT   STRAND      602    611       {ECO:0000244|PDB:4IUG}.
FT   STRAND      614    623       {ECO:0000244|PDB:4IUG}.
FT   STRAND      625    631       {ECO:0000244|PDB:4IUG}.
FT   STRAND      638    641       {ECO:0000244|PDB:4IUG}.
FT   STRAND      644    646       {ECO:0000244|PDB:4IUG}.
FT   STRAND      656    660       {ECO:0000244|PDB:4IUG}.
FT   HELIX       672    674       {ECO:0000244|PDB:4IUG}.
FT   STRAND      678    682       {ECO:0000244|PDB:4IUG}.
FT   HELIX       684    686       {ECO:0000244|PDB:4IUG}.
FT   STRAND      695    697       {ECO:0000244|PDB:4IUG}.
FT   STRAND      706    708       {ECO:0000244|PDB:4IUG}.
FT   STRAND      711    714       {ECO:0000244|PDB:4IUG}.
FT   HELIX       718    721       {ECO:0000244|PDB:4IUG}.
FT   STRAND      728    735       {ECO:0000244|PDB:4IUG}.
FT   STRAND      742    748       {ECO:0000244|PDB:4IUG}.
FT   STRAND      755    759       {ECO:0000244|PDB:4IUG}.
FT   STRAND      762    767       {ECO:0000244|PDB:4IUG}.
FT   STRAND      774    781       {ECO:0000244|PDB:4IUG}.
FT   STRAND      790    797       {ECO:0000244|PDB:4IUG}.
FT   TURN        808    810       {ECO:0000244|PDB:4IUG}.
FT   HELIX       812    814       {ECO:0000244|PDB:4IUG}.
FT   STRAND      818    824       {ECO:0000244|PDB:4IUG}.
FT   HELIX       829    831       {ECO:0000244|PDB:4IUG}.
FT   STRAND      833    839       {ECO:0000244|PDB:4IUG}.
FT   TURN        840    843       {ECO:0000244|PDB:4IUG}.
FT   TURN        848    850       {ECO:0000244|PDB:4IUG}.
FT   STRAND      852    854       {ECO:0000244|PDB:4IUG}.
FT   HELIX       859    863       {ECO:0000244|PDB:4IUG}.
FT   TURN        864    866       {ECO:0000244|PDB:4IUG}.
FT   STRAND      867    869       {ECO:0000244|PDB:4IUG}.
FT   STRAND      875    877       {ECO:0000244|PDB:4IUG}.
FT   TURN        880    882       {ECO:0000244|PDB:4IUG}.
FT   STRAND      884    899       {ECO:0000244|PDB:4IUG}.
FT   STRAND      908    912       {ECO:0000244|PDB:4IUG}.
FT   STRAND      918    925       {ECO:0000244|PDB:4IUG}.
FT   STRAND      928    934       {ECO:0000244|PDB:4IUG}.
FT   TURN        935    937       {ECO:0000244|PDB:4IUG}.
FT   STRAND      942    945       {ECO:0000244|PDB:4IUG}.
FT   STRAND      953    964       {ECO:0000244|PDB:4IUG}.
FT   STRAND      975    979       {ECO:0000244|PDB:4IUG}.
SQ   SEQUENCE   1005 AA;  109870 MW;  BAD290D2FA9EB003 CRC64;
     MKLLSVAAVA LLAAQAAGAS IKHRLNGFTI LEHPDPAKRD LLQDIVTWDD KSLFINGERI
     MLFSGEVHPF RLPVPSLWLD IFHKIRALGF NCVSFYIDWA LLEGKPGDYR AEGIFALEPF
     FDAAKEAGIY LIARPGSYIN AEVSGGGFPG WLQRVNGTLR SSDEPFLKAT DNYIANAAAA
     VAKAQITNGG PVILYQPENE YSGGCCGVKY PDADYMQYVM DQARKADIVV PFISNDASPS
     GHNAPGSGTG AVDIYGHDSY PLGFDCANPS VWPEGKLPDN FRTLHLEQSP STPYSLLEFQ
     AGAFDPWGGP GFEKCYALVN HEFSRVFYRN DLSFGVSTFN LYMTFGGTNW GNLGHPGGYT
     SYDYGSPITE TRNVTREKYS DIKLLANFVK ASPSYLTATP RNLTTGVYTD TSDLAVTPLI
     GDSPGSFFVV RHTDYSSQES TSYKLKLPTS AGNLTIPQLE GTLSLNGRDS KIHVVDYNVS
     GTNIIYSTAE VFTWKKFDGN KVLVLYGGPK EHHELAIASK SNVTIIEGSD SGIVSTRKGS
     SVIIGWDVSS TRRIVQVGDL RVFLLDRNSA YNYWVPELPT EGTSPGFSTS KTTASSIIVK
     AGYLLRGAHL DGADLHLTAD FNATTPIEVI GAPTGAKNLF VNGEKASHTV DKNGIWSSEV
     KYAAPEIKLP GLKDLDWKYL DTLPEIKSSY DDSAWVSADL PKTKNTHRPL DTPTSLYSSD
     YGFHTGYLIY RGHFVANGKE SEFFIRTQGG SAFGSSVWLN ETYLGSWTGA DYAMDGNSTY
     KLSQLESGKN YVITVVIDNL GLDENWTVGE ETMKNPRGIL SYKLSGQDAS AITWKLTGNL
     GGEDYQDKVR GPLNEGGLYA ERQGFHQPQP PSESWESGSP LEGLSKPGIG FYTAQFDLDL
     PKGWDVPLYF NFGNNTQAAR AQLYVNGYQY GKFTGNVGPQ TSFPVPEGIL NYRGTNYVAL
     SLWALESDGA KLGSFELSYT TPVLTGYGNV ESPEQPKYEQ RKGAY
//
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