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Database: UniProt
Entry: Q2UQW3
LinkDB: Q2UQW3
Original site: Q2UQW3 
ID   ROK1_ASPOR              Reviewed;         725 AA.
AC   Q2UQW3;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   16-OCT-2019, entry version 78.
DE   RecName: Full=ATP-dependent RNA helicase rok1;
DE            EC=3.6.4.13;
GN   Name=rok1; ORFNames=AO090005001076;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G.,
RA   Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K.,
RA   Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W.,
RA   Galagan J.E., Nierman W.C., Yu J., Archer D.B., Bennett J.W.,
RA   Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H.,
RA   Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R.,
RA   Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N.,
RA   Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I.,
RA   Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y.,
RA   Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y.,
RA   Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K.,
RA   Kuhara S., Ogasawara N., Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved in 40S ribosomal
CC       subunit biogenesis. Required for the processing and cleavage of
CC       35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Interacts with the U3 snoRNA and is associated with the
CC       90S and 40S pre-ribosomes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX52/ROK1
CC       subfamily. {ECO:0000305}.
DR   EMBL; AP007151; BAE56052.1; -; Genomic_DNA.
DR   RefSeq; XP_001818054.1; XM_001818002.1.
DR   PRIDE; Q2UQW3; -.
DR   EnsemblFungi; BAE56052; BAE56052; AO090005001076.
DR   GeneID; 5989999; -.
DR   KEGG; aor:AO090005001076; -.
DR   HOGENOM; HOG000242486; -.
DR   KO; K14779; -.
DR   OMA; HKIKVTD; -.
DR   Proteomes; UP000006564; Chromosome 1.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA processing.
FT   CHAIN         1    725       ATP-dependent RNA helicase rok1.
FT                                /FTId=PRO_0000232299.
FT   DOMAIN      223    434       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      474    642       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     236    243       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       179    207       Q motif.
FT   MOTIF       381    384       DEAD box.
SQ   SEQUENCE   725 AA;  80534 MW;  ED5EA5085C223242 CRC64;
     MDAFKLLTRS TKFKTGPSSS SASLPSTGKA ENPQLFRNSE AEKLLEEQKN GKKRKRGSAA
     DEPEEREPNL DFFSSNKGSS KKVVKAEEAP SDEERGSGSE DEDEMDEVQR RTILNSHKIK
     VTDMRELEEI QPVQAQGEEE PKKKKKKRKQ KEEPAQTLTK KEQKKARRLF PRPLVSFKEL
     RTQYKISRRL AENITEQGFT VPTEVQLGTL PLLLGDRTIG QSKTEEPVEP DLLVVAPTGS
     GKTLSFLIPV INKIVRHHHG QQEERGIFAV VVAPTKELAS QIVNEGRKLV SGTGVKITLM
     KKGMQVVERE DDDEDVLDEG SSESSESEDD EKTTEKKSKG KAPVTKSDIL VTTPLQLVNA
     LSANKTKPMA TLPLVRNIVL DEADVLLDPL FREQTLDIWR ACTHPELRAS LWSATMGSSI
     EDLAKSTIKE RKDASSLTKS YPLYRLVVGL KDSAIPNIQH KLVYAATEQG KLLGLRQLLH
     PAAAAASDIR LRPPFLIFTQ TIPRAVALHS ELRYDIPPEA GGSSRIAVLH SDLSDGQRSE
     IMKNFRKGEI WILVTTDLLA RGIDFRGING VVNYDIPNSA AVYVHRVGRT GRAGREGGVA
     VTYYTKEDIP YVKSIANVID VSEKLRGTEG EKSVQKWLLD SLPDLSKKDK KELKKHGVRA
     RQTNLKSVAD DKQQRKTRIS TKSGFERRME NKKKGAIAAS RNRKLQGPSG AEPDSGDDGW
     GGIQE
//
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