ID Q2USV7_ASPOR Unreviewed; 2085 AA.
AC Q2USV7;
DT 24-JAN-2006, integrated into UniProtKB/TrEMBL.
DT 24-JAN-2006, sequence version 1.
DT 22-FEB-2023, entry version 94.
DE RecName: Full=separase {ECO:0000256|ARBA:ARBA00012489};
DE EC=3.4.22.49 {ECO:0000256|ARBA:ARBA00012489};
GN ORFNames=AO090005000276 {ECO:0000313|EMBL:BAE55358.1};
OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=510516 {ECO:0000313|EMBL:BAE55358.1, ECO:0000313|Proteomes:UP000006564};
RN [1] {ECO:0000313|EMBL:BAE55358.1, ECO:0000313|Proteomes:UP000006564}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 42149 / RIB 40 {ECO:0000313|Proteomes:UP000006564};
RX PubMed=16372010; DOI=10.1038/nature04300;
RA Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA Takahashi M., Takase K., Terabayashi Y., Wortman J., Yamada O.,
RA Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA Kikuchi H.;
RT "Genome sequencing and analysis of Aspergillus oryzae.";
RL Nature 438:1157-1161(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=All bonds known to be hydrolyzed by this endopeptidase have
CC arginine in P1 and an acidic residue in P4. P6 is often occupied by
CC an acidic residue or by a hydroxy-amino-acid residue, the
CC phosphorylation of which enhances cleavage.; EC=3.4.22.49;
CC Evidence={ECO:0000256|ARBA:ARBA00000451};
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DR EMBL; AP007151; BAE55358.1; -; Genomic_DNA.
DR RefSeq; XP_001817360.1; XM_001817308.1.
DR STRING; 510516.Q2USV7; -.
DR EnsemblFungi; BAE55358; BAE55358; AO090005000276.
DR GeneID; 5989305; -.
DR KEGG; aor:AO090005000276; -.
DR HOGENOM; CLU_000454_0_0_1; -.
DR OMA; CAVTFLM; -.
DR OrthoDB; 5479815at2759; -.
DR Proteomes; UP000006564; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0098813; P:nuclear chromosome segregation; IEA:UniProt.
DR GO; GO:0000280; P:nuclear division; IEA:UniProt.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 1.
DR InterPro; IPR005314; Peptidase_C50.
DR InterPro; IPR030397; SEPARIN_core_dom.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR12792; EXTRA SPINDLE POLES 1-RELATED; 1.
DR PANTHER; PTHR12792:SF0; SEPARIN; 1.
DR Pfam; PF03568; Peptidase_C50; 1.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; TPR-like; 2.
DR PROSITE; PS51700; SEPARIN; 1.
PE 4: Predicted;
KW Chromosome partition {ECO:0000256|ARBA:ARBA00022829};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000006564}.
FT DOMAIN 1906..2001
FT /note="Peptidase C50"
FT /evidence="ECO:0000259|PROSITE:PS51700"
FT REGION 44..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 231..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1337..1376
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1461..1496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..77
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..254
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1341..1365
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1461..1478
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2085 AA; 229991 MW; 3B16EC4CDB86BAE1 CRC64;
MAVTTLLSDS SVESVKQAVR STSTCSNATV ISLQTLFRGL SKTAPESDKT TVKRGTRTTK
TTATSTRPKS SRGTKISAQE AAINTVMEMD AARLSFQEKL VLATDVFNTT LKTLSDASKQ
CAKRDDARVR TACASPSTSH GVKSPRKLKT SQEDELDAGL VAVAECASTA LSSLRSLKGD
QVDQQDALPN MQLEQGACVL AGRYLSLGLN DMASKELRGL KRRLQQHLDN QATVRPKTTS
RRKNEAQEEE NTKERMSDFL TFANFDHAGP VLSVLVPFQS NALRLIASEK KLATVQKVAS
SLQLAELSSP ANVIVAATES GALTKDKAAL QLQLLSNTVL SLAGVHQPST SSTTRDRLKP
FTSLSLQLLS LEIRCMSWKL SGHTCDEGKE MWDPLTRYLA NYSHHSKSIE KAEFASIYKT
IVRLQTAVAN TQKRTTASLR DNVSVARIAT ILGQLAQEAG CFDEALKLFT EAVNPLSSGQ
CLSLATVRCK IASLHLQASK SSKPSLDKVT NVVSDATSSL SMPLKGSLSD LDELLVEAAK
LKKLTMSAYG DLATKAGEKN GIDISQIYEY LPAFLRFLRR YVGRPSSSDN ESKEDDLFLT
RVRACRNIVL SAVDSALAVG KFSVMSQRPP WEDVLSALTD CQRLLTAIDI ADKEADPSVL
EQIGAAFVRL SNLFWSRYLK EKERGKGYRE LVPLVKHSAN LLSSCSAEQR NTGFAALKYE
RLAYLYMEGN RGAEAEQAFS QAIQEHIDTG VLDGLISSPN FPYRTSQDPK SSGFMLGRVL
TAYLKLQLKL RGKSKSNGFF DDESQSLLVR GHIMEWQMGI LTELHSHASS DEAFRSHFTC
LVSKLLGLYD PEVYPIRRLR VVLGGLRSSL ERPNCLESTV LQDLLEEALE AIELGDYAED
GALATLATHL HNSVRLSVGL LQGDLQPESL NLMISSWTSL MRNCNDGVTL IQYVGDDEHY
LLQLKAVVDY TEIHGLWKLQ LSTLELVLRV TELQEHGDFS EAIIVIARLV QQNCRLGYCK
KAGDLLTRAE QYLGPNVSCL ATLSHKLAGV AYLLETGETD KAAANLSTAR ILYEKNQKKQ
DLSSCSVLAK IAWERLVADA AFMSSQLSFA QGVIKDALFF AKLSVRLNCR IWAKVEKISQ
RKQEKSLPVP VISVGDSSEL DNIVETMAKL DVSQANHTTT SVYTQGAPFW PHIGSHHTSL
LNLASLSAHH GLFQDAIYYG EQALKINKTL NAPVRLINSQ AQLGSHWILG GHISEGQELL
TAAEKLSKQL ESSVELASLQ MGLASLYRAQ GHYRDELQAL LEADRIMADF GSLDVSDVAS
VSSGVLELED KMESLRIRPS SRRTKQPAAT TRRTRATTSA RKPTKVDATP SKAADTVPES
KSLLQIRSDI LRQQAACCRS LRDFERASCL LNNARQYAVS RDSQISVHLG ESEHFLAEAI
RHFASHAVYC VLPESTISLP SLQSPSKTTS TSKAATKSTA RKPRAPARGT RSRAQTASED
FAVMLSKAGD CLNNVFATAT TVGSTLDSHS ASRLMSRISM LSHVTAAESI SAWSQPPANV
NELGRIGAFA REHTAIDLDK QLAEYNDPLL WPTPSPMMAE SEDLCSSRFV EEYIDILPDN
WNVLSLSLSA DRSEFIVSRL HQGRSPFLLR LPLKRGNSEE EDEQFTFDDG REEMQELIKL
ANESSHAAKA QTDRQMKKDW WKNREALDRR METLLQNIEN VWFGGFRGIF SPVPHDTTSL
TRFASSFQSI LDKHLPSRQK GGRAEGPRLT LHPNVLELFI GVADLDDQED PEETLMDLLY
FVVDILQFQG ERNAYDEIDF DMMVVETLDA VRAYHDATSN RRGKPTPNHT VLVLDKSLHL
FPWESLPCLQ GFPVCRVPSL ECLRDRVLQF QNKRNSDLAR GVGIDRNSGT YILNPTGDLR
TTQSTFEKDL SELQGWTGIM QREPSEEEFK RGLETTNLFL YFGHGSGAQY IRGRTVKRLE
QCAVAFLMGC SSGTLTEAGE YEPYGTPMNY LHAGSPALVA TLWDVTDKDI DRFAQSTFEK
WGLFDAGSDG ATLDEAVSQS RPACVLKYLN GAAPVIYGVP SVFLE
//
