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Database: UniProt
Entry: Q381F9
LinkDB: Q381F9
Original site: Q381F9 
ID   XPBR_TRYB2              Reviewed;         777 AA.
AC   Q381F9;
DT   31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   16-OCT-2019, entry version 83.
DE   RecName: Full=DNA repair helicase XPB-R {ECO:0000303|PubMed:24134817};
DE            EC=3.6.4.12;
GN   Name=XPB-R {ECO:0000303|PubMed:24134817};
GN   ORFNames=Tb11.01.7950, Tb927.11.16270;
OS   Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=185431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=927/4 GUTat10.1;
RX   PubMed=16020726; DOI=10.1126/science.1112642;
RA   Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA   Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B.,
RA   Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L.,
RA   Wickstead B., Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J.,
RA   Bringaud F., Brooks K., Carrington M., Cherevach I.,
RA   Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A.,
RA   Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C.,
RA   Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H.,
RA   Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K.,
RA   Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V.,
RA   Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M.,
RA   Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA   Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E.,
RA   Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S.,
RA   Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A.,
RA   Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B.,
RA   White O., Whitehead S., Woodward J., Wortman J., Adams M.D.,
RA   Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H.,
RA   Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M.,
RA   Melville S.E., El-Sayed N.M.A.;
RT   "The genome of the African trypanosome Trypanosoma brucei.";
RL   Science 309:416-422(2005).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24134817; DOI=10.1111/mmi.12435;
RA   Badjatia N., Nguyen T.N., Lee J.H., Guenzl A.;
RT   "Trypanosoma brucei harbours a divergent XPB helicase paralogue that
RT   is specialized in nucleotide excision repair and conserved among
RT   kinetoplastid organisms.";
RL   Mol. Microbiol. 90:1293-1308(2013).
CC   -!- FUNCTION: ATP-dependent 3'-5' DNA helicase involved in nucleotide
CC       excision repair (NER) of damaged DNA. XPB-R is a paralog of XBP,
CC       but is not a component of the TFIIH basal transcription factor and
CC       is dispensable for RNA polymerase II transcription.
CC       {ECO:0000269|PubMed:16020726}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- DISRUPTION PHENOTYPE: Mutants are much less tolerant than wild-
CC       type cells to UV light- and cisplatin-induced DNA damage.
CC       {ECO:0000269|PubMed:24134817}.
CC   -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
CC       {ECO:0000305}.
DR   EMBL; CH464491; EAN80572.1; -; Genomic_DNA.
DR   RefSeq; XP_829684.1; XM_824591.1.
DR   STRING; 5691.EAN80572; -.
DR   PaxDb; Q381F9; -.
DR   GeneDB; Tb927.11.16270:pep; -.
DR   GeneID; 3664517; -.
DR   KEGG; tbr:Tb11.01.7950; -.
DR   EuPathDB; TriTrypDB:Tb927.11.16270; -.
DR   HOGENOM; HOG000259918; -.
DR   InParanoid; Q381F9; -.
DR   KO; K10843; -.
DR   Proteomes; UP000008524; Chromosome 11 Scaffold 1.
DR   GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR   GO; GO:0000112; C:nucleotide-excision repair factor 3 complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISM:GeneDB.
DR   GO; GO:0005675; C:transcription factor TFIIH holo complex; ISA:GeneDB.
DR   GO; GO:0097550; C:transcriptional preinitiation complex; IBA:GO_Central.
DR   GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003684; F:damaged DNA binding; ISA:GeneDB.
DR   GO; GO:0008134; F:transcription factor binding; IBA:GO_Central.
DR   GO; GO:0006281; P:DNA repair; ISA:GeneDB.
DR   GO; GO:0033683; P:nucleotide-excision repair, DNA incision; IBA:GO_Central.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:GOC.
DR   GO; GO:0009411; P:response to UV; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR   GO; GO:0006351; P:transcription, DNA-templated; ISA:GeneDB.
DR   InterPro; IPR032438; ERCC3_RAD25_C.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR032830; XPB/Ssl2_N.
DR   Pfam; PF16203; ERCC3_RAD25_C; 1.
DR   Pfam; PF13625; Helicase_C_3; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN         1    777       DNA repair helicase XPB-R.
FT                                /FTId=PRO_0000442799.
FT   DOMAIN      212    416       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      484    631       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     225    232       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       369    372       DEVH box. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
SQ   SEQUENCE   777 AA;  85938 MW;  FD0922B05C7FCC27 CRC64;
     MQCLFLEERI KKEGCVVLFA EAFRPSFANI QGFLTTIAEP VSRLSLMHEY RMTYFSLGAA
     ASASISVSEV IQFLDDHVYF FRDKCFASYR ESVCAFAEMY MSRCNLARVV IDESRTLLEC
     KDIVTAKTLL KDRVVRSLCC QPKETVGEDA CPHFLLKSRA AARVVAERCV LLGYPLQQQY
     EYEKDTSIRN VNIALKSQTR PRPYQIAAVD AAASDGALRS GCIVLPCGSG KTLVGIMLLC
     KVKKPTLILC AGGVSVEQWR NQILEFASVC APANNEEGDP SNSTTGEKVR TAAVGAARIS
     CLTGKQKDEI TDETDIVLTT YSMLVTAHKA QARCQVEGFE MNADGRGRNP RRANPKERLF
     QPYGLLIMDE VHMMPADAYK DSLGFINAKG VVGLTATYVR EDSKIRDLFH LVGPKLFDIS
     WERLASSGYL AHVTCIEVLT PLARRFSLEY LERSSELTSP QHGTPLLVML AAANPNKMLC
     VMEIVKRHVA ESSKILVFCD HIMLLKEYSK LLGAPVVCGD TPHRERLMIF SDFQSTSKVN
     VVCLSRVGDV SVNLPSANVV VQVSSHGGSR RQEAQRLGRI LRPKEKASNG KPTDAWFYTV
     ISTDTVEMSY AAHRTAFLVD QGYTCSVTEF NPDGAPEAAV EGVDDTAPGD VVSIRQAKLR
     GTFKKQELKC SVASPTAQGS VNPRSLDYQE KLLCRVVASW ELDYQNATSQ QNEPGVANDT
     ATGLIDKKMK RARDETAEDI KREWNSGAQT TQPRGDFCRL PLQRLVGAND DVVYHES
//
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