ID Q38CA1_TRYB2 Unreviewed; 234 AA.
AC Q38CA1;
DT 22-NOV-2005, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2005, sequence version 1.
DT 27-MAR-2024, entry version 101.
DE SubName: Full=Hypoxanthine-guanine phosphoribosyltransferase, putative {ECO:0000313|EMBL:EAN77569.1};
GN ORFNames=Tb10.70.6660 {ECO:0000313|EMBL:EAN77569.1};
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431 {ECO:0000313|EMBL:EAN77569.1, ECO:0000313|Proteomes:UP000008524};
RN [1] {ECO:0000313|EMBL:EAN77569.1, ECO:0000313|Proteomes:UP000008524}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:EAN77569.1,
RC ECO:0000313|Proteomes:UP000008524};
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [2] {ECO:0007829|PDB:6AQO, ECO:0007829|PDB:6AR9}
RP X-RAY CRYSTALLOGRAPHY (2.28 ANGSTROMS) OF 2-234 IN COMPLEX WITH MG(2+).
RX PubMed=29481567; DOI=10.1371/journal.pntd.0006301;
RA Dolezelova E., Teran D., Gahura O., Kotrbova Z., Prochazkova M., Keough D.,
RA Spacek P., Hockova D., Guddat L., Zikova A.;
RT "Evaluation of the Trypanosoma brucei 6-oxopurine salvage pathway as a
RT potential target for drug discovery.";
RL PLoS Negl. Trop. Dis. 12:e0006301-e0006301(2018).
RN [3] {ECO:0007829|PDB:6MXB, ECO:0007829|PDB:6MXC}
RP X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF 2-234 IN COMPLEX WITH GMP; IMP;
RP MG(2+) AND XMP.
RX PubMed=31287615; DOI=10.1111/febs.14987;
RA Teran D., Dolezelova E., Keough D.T., Hockova D., Zikova A., Guddat L.W.;
RT "Crystal structures of Trypanosoma brucei hypoxanthine - guanine - xanthine
RT phosphoribosyltransferase in complex with IMP, GMP and XMP.";
RL FEBS J. 286:4721-4736(2019).
RN [4] {ECO:0007829|PDB:7SCR}
RP X-RAY CRYSTALLOGRAPHY (2.12 ANGSTROMS) OF 2-234.
RX PubMed=35175749; DOI=10.1021/acs.jmedchem.1c01881;
RA Klejch T., Keough D.T., King G., Dolezelova E., Cesnek M., Budesinsky M.,
RA Zikova A., Janeba Z., Guddat L.W., Hockova D.;
RT "Stereo-Defined Acyclic Nucleoside Phosphonates are Selective and Potent
RT Inhibitors of Parasite 6-Oxopurine Phosphoribosyltransferases.";
RL J. Med. Chem. 65:4030-4057(2022).
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DR EMBL; CM000208; EAN77569.1; -; Genomic_DNA.
DR RefSeq; XP_822397.1; XM_817304.1.
DR PDB; 6AQO; X-ray; 2.64 A; A/B/C/D/E/F=2-234.
DR PDB; 6AR9; X-ray; 2.28 A; A/B/C/D/E/F=2-234.
DR PDB; 6MXB; X-ray; 2.19 A; A/B/C/D/E/F=2-234.
DR PDB; 6MXC; X-ray; 1.99 A; A/B/C/D/E/F=2-234.
DR PDB; 6MXD; X-ray; 2.96 A; A/B/C/D=2-234.
DR PDB; 7SCR; X-ray; 2.12 A; A/B/C/D/E/F=2-234.
DR PDBsum; 6AQO; -.
DR PDBsum; 6AR9; -.
DR PDBsum; 6MXB; -.
DR PDBsum; 6MXC; -.
DR PDBsum; 6MXD; -.
DR AlphaFoldDB; Q38CA1; -.
DR SMR; Q38CA1; -.
DR STRING; 185431.Q38CA1; -.
DR PaxDb; 5691-EAN77569; -.
DR GeneID; 3662981; -.
DR KEGG; tbr:Tb10.70.6660; -.
DR VEuPathDB; TriTrypDB:Tb927.10.1390; -.
DR eggNOG; KOG3367; Eukaryota.
DR InParanoid; Q38CA1; -.
DR OMA; PKHYEND; -.
DR OrthoDB; 167978at2759; -.
DR Proteomes; UP000008524; Chromosome 10.
DR GO; GO:0005737; C:cytoplasm; ISA:GeneDB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0020015; C:glycosome; IDA:GeneDB.
DR GO; GO:0052657; F:guanine phosphoribosyltransferase activity; IDA:GeneDB.
DR GO; GO:0004422; F:hypoxanthine phosphoribosyltransferase activity; IDA:GeneDB.
DR GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR GO; GO:0042301; F:phosphate ion binding; IDA:GeneDB.
DR GO; GO:0000310; F:xanthine phosphoribosyltransferase activity; IDA:GeneDB.
DR GO; GO:0032263; P:GMP salvage; IBA:GO_Central.
DR GO; GO:0006178; P:guanine salvage; IBA:GO_Central.
DR GO; GO:0046100; P:hypoxanthine metabolic process; IBA:GO_Central.
DR GO; GO:0032264; P:IMP salvage; IBA:GO_Central.
DR CDD; cd06223; PRTases_typeI; 1.
DR Gene3D; 3.40.50.2020; -; 1.
DR InterPro; IPR000836; PRibTrfase_dom.
DR InterPro; IPR029057; PRTase-like.
DR PANTHER; PTHR43340:SF1; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; 1.
DR PANTHER; PTHR43340; HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE; 1.
DR Pfam; PF00156; Pribosyltran; 1.
DR SUPFAM; SSF53271; PRTase-like; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:6AQO, ECO:0007829|PDB:6AR9};
KW Glycosyltransferase {ECO:0000313|EMBL:EAN77569.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0007829|PDB:6AQO};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000008524};
KW Transferase {ECO:0000313|EMBL:EAN77569.1}.
FT DOMAIN 76..205
FT /note="Phosphoribosyltransferase"
FT /evidence="ECO:0000259|Pfam:PF00156"
FT BINDING 147
FT /ligand="IMP"
FT /ligand_id="ChEBI:CHEBI:58053"
FT /evidence="ECO:0007829|PDB:6MXD"
FT BINDING 147
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:6AQO, ECO:0007829|PDB:6AR9"
FT BINDING 147
FT /ligand="XMP"
FT /ligand_id="ChEBI:CHEBI:57464"
FT /evidence="ECO:0007829|PDB:6MXB"
FT BINDING 148
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007829|PDB:6MXC"
FT BINDING 148
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:6AQO, ECO:0007829|PDB:6AR9"
FT BINDING 151
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007829|PDB:6MXC"
FT BINDING 151
FT /ligand="IMP"
FT /ligand_id="ChEBI:CHEBI:58053"
FT /evidence="ECO:0007829|PDB:6MXD"
FT BINDING 151
FT /ligand="XMP"
FT /ligand_id="ChEBI:CHEBI:57464"
FT /evidence="ECO:0007829|PDB:6MXB"
FT BINDING 152
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007829|PDB:6MXC"
FT BINDING 152
FT /ligand="IMP"
FT /ligand_id="ChEBI:CHEBI:58053"
FT /evidence="ECO:0007829|PDB:6MXD"
FT BINDING 152
FT /ligand="XMP"
FT /ligand_id="ChEBI:CHEBI:57464"
FT /evidence="ECO:0007829|PDB:6MXB"
FT BINDING 153
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007829|PDB:6MXC"
FT BINDING 153
FT /ligand="IMP"
FT /ligand_id="ChEBI:CHEBI:58053"
FT /evidence="ECO:0007829|PDB:6MXD"
FT BINDING 153
FT /ligand="XMP"
FT /ligand_id="ChEBI:CHEBI:57464"
FT /evidence="ECO:0007829|PDB:6MXB"
FT BINDING 154
FT /ligand="IMP"
FT /ligand_id="ChEBI:CHEBI:58053"
FT /evidence="ECO:0007829|PDB:6MXD"
FT BINDING 155
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007829|PDB:6MXC"
FT BINDING 155
FT /ligand="IMP"
FT /ligand_id="ChEBI:CHEBI:58053"
FT /evidence="ECO:0007829|PDB:6MXD"
FT BINDING 155
FT /ligand="XMP"
FT /ligand_id="ChEBI:CHEBI:57464"
FT /evidence="ECO:0007829|PDB:6MXB"
FT BINDING 180
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007829|PDB:6MXC"
FT BINDING 180
FT /ligand="IMP"
FT /ligand_id="ChEBI:CHEBI:58053"
FT /evidence="ECO:0007829|PDB:6MXD"
FT BINDING 180
FT /ligand="XMP"
FT /ligand_id="ChEBI:CHEBI:57464"
FT /evidence="ECO:0007829|PDB:6MXB"
FT BINDING 201
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007829|PDB:6MXC"
FT BINDING 201
FT /ligand="IMP"
FT /ligand_id="ChEBI:CHEBI:58053"
FT /evidence="ECO:0007829|PDB:6MXD"
FT BINDING 202
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0007829|PDB:6MXC"
FT BINDING 202
FT /ligand="IMP"
FT /ligand_id="ChEBI:CHEBI:58053"
FT /evidence="ECO:0007829|PDB:6MXD"
FT BINDING 202
FT /ligand="XMP"
FT /ligand_id="ChEBI:CHEBI:57464"
FT /evidence="ECO:0007829|PDB:6MXB"
FT BINDING 208
FT /ligand="XMP"
FT /ligand_id="ChEBI:CHEBI:57464"
FT /evidence="ECO:0007829|PDB:6MXB"
SQ SEQUENCE 234 AA; 26360 MW; 617FDDB5B21BFE5D CRC64;
MHSGHPLKPN FVGRDADGNV TVDGRSYPMA ESVVATESTI HRSMKEMAQT LANAYKTLKH
RDTHNKGNSA LAPITDENPL IIISVLKGSY IFTADMVRYL GDCGLPNVVD FIRITSYRGT
TKSSGTVQVL DNLRFTELTG KHVLIMEDIA DTGRTMKLLV EKIRREYRPA SLKVCVLVDK
PGGRVVDFKP EFVCLTAPTR YVVGYGFEVN DRYRNYRHVF VLKPEYAKRY PSKL
//
