GenomeNet

Database: UniProt
Entry: Q38F76
LinkDB: Q38F76
Original site: Q38F76 
ID   IF4A_TRYB2              Reviewed;         404 AA.
AC   Q38F76;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   31-JUL-2019, entry version 85.
DE   RecName: Full=Probable eukaryotic initiation factor 4A;
DE            Short=eIF-4A;
DE            EC=3.6.4.13;
DE   AltName: Full=ATP-dependent RNA helicase eIF4A;
GN   ORFNames=Tb09.160.3270;
OS   Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=185431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX   PubMed=16020726; DOI=10.1126/science.1112642;
RA   Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA   Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B.,
RA   Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L.,
RA   Wickstead B., Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J.,
RA   Bringaud F., Brooks K., Carrington M., Cherevach I.,
RA   Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A.,
RA   Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C.,
RA   Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H.,
RA   Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K.,
RA   Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V.,
RA   Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M.,
RA   Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA   Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E.,
RA   Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S.,
RA   Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A.,
RA   Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B.,
RA   White O., Whitehead S., Woodward J., Wortman J., Adams M.D.,
RA   Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H.,
RA   Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M.,
RA   Melville S.E., El-Sayed N.M.A.;
RT   "The genome of the African trypanosome Trypanosoma brucei.";
RL   Science 309:416-422(2005).
CC   -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the
CC       eIF4F complex involved in cap recognition and is required for mRNA
CC       binding to ribosome. In the current model of translation
CC       initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR
CC       of mRNAs which is necessary to allow efficient binding of the
CC       small ribosomal subunit, and subsequent scanning for the initiator
CC       codon (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of
CC       which varies with external and internal environmental conditions.
CC       It is composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A
CC       subfamily. {ECO:0000305}.
DR   EMBL; CM000207; EAN76544.1; -; Genomic_DNA.
DR   RefSeq; XP_803774.1; XM_798681.1.
DR   SMR; Q38F76; -.
DR   STRING; 5691.EAN76544; -.
DR   PaxDb; Q38F76; -.
DR   GeneDB; Tb927.9.4680:pep; -.
DR   GeneID; 3660503; -.
DR   KEGG; tbr:Tb09.160.3270; -.
DR   EuPathDB; TriTrypDB:Tb927.9.4680; -.
DR   HOGENOM; HOG000268797; -.
DR   InParanoid; Q38F76; -.
DR   KO; K03257; -.
DR   OMA; RNPIRIL; -.
DR   Proteomes; UP000008524; Chromosome 9.
DR   GO; GO:0005737; C:cytoplasm; ISA:GeneDB.
DR   GO; GO:0010494; C:cytoplasmic stress granule; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; ISA:GeneDB.
DR   GO; GO:0016281; C:eukaryotic translation initiation factor 4F complex; ISO:GeneDB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003729; F:mRNA binding; IDA:GeneDB.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; ISO:GeneDB.
DR   GO; GO:0008186; F:RNA-dependent ATPase activity; ISO:GeneDB.
DR   GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR   GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome; RNA-binding.
FT   CHAIN         1    404       Probable eukaryotic initiation factor 4A.
FT                                /FTId=PRO_0000291644.
FT   DOMAIN       57    231       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      242    402       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      70     77       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF        26     54       Q motif.
FT   MOTIF       179    182       DEAD box.
SQ   SEQUENCE   404 AA;  45362 MW;  AB30F42D7CD7C717 CRC64;
     MAQQGKVEPQ DQDSFLDDQP GIRPIPSFDD MPLHQNLLRG IYSHGFEKPS SIQQRAIVPF
     TRGGDIIAQA QSGTGKTGAF SIGLLQRLDF RHNVLQGLVL SPTRELAMQT AEVITRIGEF
     LAEGSSSFCA TFVGGTRVQD DYRKLQSGTI VAVGTPGRVV DVTKRGAMRT ESLRVLVLDE
     ADEMLSQGFA EQIYDIFRFL PKEIQVALFS ATMPDDVLEL TKKFMRDPTR ILVKRESLTL
     EGIKQFFIAV EEEHKLDTLM DLYETVSIAQ SVIFANTRRK VDWLASQLNS SNHTVSCMHS
     EMSKQEREKV MGTFRNGSSR VLVTTDLVAR GIDVHHVNIV INFDLPTNKE NYLHRIGRGG
     RYGRKGVAIN FVTQKDVEVL REIESHYHTQ IEELPVDFAA YLGE
//
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