ID Q3Z790_DEHM1 Unreviewed; 738 AA.
AC Q3Z790;
DT 27-SEP-2005, integrated into UniProtKB/TrEMBL.
DT 27-SEP-2005, sequence version 1.
DT 27-MAR-2024, entry version 111.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=pcrA {ECO:0000313|EMBL:AAW39558.1};
GN OrderedLocusNames=DET1196 {ECO:0000313|EMBL:AAW39558.1};
OS Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195)
OS (Dehalococcoides ethenogenes (strain 195)).
OC Bacteria; Chloroflexota; Dehalococcoidia; Dehalococcoidales;
OC Dehalococcoidaceae; Dehalococcoides.
OX NCBI_TaxID=243164 {ECO:0000313|EMBL:AAW39558.1, ECO:0000313|Proteomes:UP000008289};
RN [1] {ECO:0000313|EMBL:AAW39558.1, ECO:0000313|Proteomes:UP000008289}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-2266 / KCTC 15142 / 195
RC {ECO:0000313|Proteomes:UP000008289};
RX PubMed=15637277; DOI=10.1126/science.1102226;
RA Seshadri R., Adrian L., Fouts D.E., Eisen J.A., Phillippy A.M., Methe B.A.,
RA Ward N.L., Nelson W.C., Deboy R.T., Khouri H.M., Kolonay J.F., Dodson R.J.,
RA Daugherty S.C., Brinkac L.M., Sullivan S.A., Madupu R., Nelson K.E.,
RA Kang K.H., Impraim M., Tran K., Robinson J.M., Forberger H.A., Fraser C.M.,
RA Zinder S.H., Heidelberg J.F.;
RT "Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides
RT ethenogenes.";
RL Science 307:105-108(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP000027; AAW39558.1; -; Genomic_DNA.
DR RefSeq; WP_010936886.1; NC_002936.3.
DR AlphaFoldDB; Q3Z790; -.
DR STRING; 243164.DET1196; -.
DR KEGG; det:DET1196; -.
DR PATRIC; fig|243164.10.peg.1129; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_2_0; -.
DR InParanoid; Q3Z790; -.
DR Proteomes; UP000008289; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 6..285
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 286..563
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 27..34
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 738 AA; 83226 MW; 86586DC72B5571AD CRC64;
MQDILTGLNP AQKQAVEAVE GPVLILAGPG SGKTRVITHR IAYLIKVVGI NPHRIMAVTF
TNKAAREMET RLNLLAPSAA GRLTMGTFHA ICARILRQDG LPLGVPADFV IYDDDDQQSL
IKQAMAELEL DPKRYPPASI RNAISQAKSQ LVLPAYYFEK GRSYFEEVVG RVYELYEKRL
KQNKALDFDD LLLKVVQLFK QHPEVLARYQ ERYLHVMVDE FQDTNLVQYE LVKMLSAKYK
NICVVGDPDQ SIYSWRSADL RNVFNFETDN PGAKVILLEQ NYRSTKNILE AASCVIAPNS
GRKPISLWTE NESGQPIAVV ETFNEQEEAQ FVVSEIEKLT RYEKFSPGDI AILYRTNAQS
RALEEAFIRY GMPYRLVSGT RFYQRREVKD IVAYLRLLQN PQDSVSLQRV INIPTRGIGA
KTLSDLGQFA REQDISLYES LVNLSDENTP KPPIPSRGLK PLTDFGLLLK SLVADARNLP
LLNLLDRLLE GINYREFLFA SEDGEERWDN ILELRTVAQQ YQEIVPPEGL SVFLESVALV
SDLDSLEEGV SAVTLITLHQ AKGLEYPVVF MVGMEEGVLP HSRSFDDPAQ MQEERRLCYV
GVTRARKRLY LVRAFRRSLM GQSNVNEPSR YLKDIPQSIT KGREKQPLSE QHTAASAVSP
VIKQHLQSAG MSIKSASPPA PKLLELEAGD RVLHPQFGEG IVTSINRVKN DAEVMISFHS
VGMKRLMLSF ARLTKLEN
//
