ID Q44289_NOSS1 Unreviewed; 746 AA.
AC Q44289;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 144.
DE SubName: Full=NarB protein {ECO:0000313|EMBL:AAB40292.1};
DE SubName: Full=Nitrate reductase {ECO:0000313|EMBL:BAB72570.1};
GN Name=narB {ECO:0000313|EMBL:AAB40292.1};
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690 {ECO:0000313|EMBL:AAB40292.1};
RN [1] {ECO:0000313|EMBL:AAB40292.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PCC7120 {ECO:0000313|EMBL:AAB40292.1};
RX PubMed=8982006;
RA Cai Y., Wolk C.P.;
RT "Nitrogen deprivation of Anabaena sp. strain PCC 7120 elicits rapid
RT activation of a gene cluster that is essential for uptake and utilization
RT of nitrate.";
RL J. Bacteriol. 179:258-266(1997).
RN [2] {ECO:0000313|EMBL:BAB72570.1, ECO:0000313|Proteomes:UP000002483}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 {ECO:0000313|EMBL:BAB72570.1}, and PCC 7120 / SAG
RC 25.82 / UTEX 2576 {ECO:0000313|Proteomes:UP000002483};
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Nitrogen metabolism. {ECO:0000256|ARBA:ARBA00004909}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. NasA/NapA/NarB subfamily.
CC {ECO:0000256|ARBA:ARBA00008747}.
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DR EMBL; L49163; AAB40292.1; -; Genomic_DNA.
DR EMBL; BA000019; BAB72570.1; -; Genomic_DNA.
DR PIR; AC1883; AC1883.
DR RefSeq; WP_010994788.1; NZ_RSCN01000009.1.
DR AlphaFoldDB; Q44289; -.
DR STRING; 103690.gene:10492623; -.
DR KEGG; ana:alr0612; -.
DR eggNOG; COG0243; Bacteria.
DR OrthoDB; 9805142at2; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd02791; MopB_CT_Nitrate-R-NapA-like; 1.
DR CDD; cd02754; MopB_Nitrate-R-NapA-like; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.40.50.740; -; 1.
DR Gene3D; 2.20.25.90; ADC-like domains; 1.
DR Gene3D; 3.40.228.10; Dimethylsulfoxide Reductase, domain 2; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR041957; CT_Nitrate-R-NapA-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR PANTHER; PTHR43105:SF9; NITRATE REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15190)-RELATED; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR Pfam; PF04879; Molybdop_Fe4S4; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR PIRSF; PIRSF000144; CbbBc; 2.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000002483}.
FT DOMAIN 2..70
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
FT REGION 27..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 746 AA; 83013 MW; F44CD4A5DFE9E2C2 CRC64;
MTESTKTLCP YCGVGCGLEV TPPAQLNKAT NRDSQGNPTW RVRGDKAHPS SQGMVCVKGA
TIAESLDKNR LHYPMVRESL DQEFRRVSWD EAFDLITKRI QSVRFTQGAE AICMYGSGQF
QTEDYYIAQK LMKGCLGSNN FDANSRLCMS SAVSGYIQSF GADGPPCCYE DLELTDCAFL
IGTNTAECHP IVFNRLEKYH RKNHKVKMIV VDPRRTPTAE AADLHLAIKP GTDIDLLNGI
AHLLMRWNMI DVGFIDDCTR NFSAYAEVIR HYSPEVVARQ CGITIEDLET ASRYWGESQR
VLSLWSMGVN QSSEGTAKVR TIINLHLMTG QVGKPGAGPF SLTGQPNAMG GREAGGLAHL
LPGYRLVKNP QHRAELEAFW GFQPGQISPH PGLTAWDMIT GLEDGNVGLL WIAATNPAVS
MPDLERTKKA LLRSPFTIYQ DAYHPTETTA YAHVLLPAAQ WGEKTGIMTN SERRVTLCQA
FRQPPREAKP DWEIFAEVGR RLGFEDKFAF TNSAQVYAEF TQLTKGRPCD MSGISHQQLQ
AQGPTQWPHS SINIETQKPT QHPTVNSQQS TVTSQQSKRL YTDLRFHTPD GRARFGAYYS
KGLAEPPDPS YPFVLTTGRL YGHWHTQTRT GRIEKIRAMH PEPFIEIHPR DAAKLGITDN
VVVEVRSRRG SAKFPAKVTK AISPGTVFVP MHWGKLWADD AEANALTHSE SCPDSLQPEL
KACAVQLIPI SVENTAQNYQ LQSSQW
//
