ID WDR5_RAT Reviewed; 334 AA.
AC Q498M4;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 27-MAR-2024, entry version 142.
DE RecName: Full=WD repeat-containing protein 5;
GN Name=Wdr5;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP INTERACTION WITH PER1.
RX PubMed=15860628; DOI=10.1126/science.1107373;
RA Brown S.A., Ripperger J., Kadener S., Fleury-Olela F., Vilbois F.,
RA Rosbash M., Schibler U.;
RT "PERIOD1-associated proteins modulate the negative limb of the mammalian
RT circadian oscillator.";
RL Science 308:693-696(2005).
CC -!- FUNCTION: Contributes to histone modification (By similarity). May
CC position the N-terminus of histone H3 for efficient trimethylation at
CC 'Lys-4' (By similarity). As part of the MLL1/MLL complex it is involved
CC in methylation and dimethylation at 'Lys-4' of histone H3 (By
CC similarity). H3 'Lys-4' methylation represents a specific tag for
CC epigenetic transcriptional activation (By similarity). As part of the
CC NSL complex it may be involved in acetylation of nucleosomal histone H4
CC on several lysine residues (By similarity). May regulate osteoblasts
CC differentiation (By similarity). In association with RBBP5 and ASH2L,
CC stimulates the histone methyltransferase activities of KMT2A, KMT2B,
CC KMT2C, KMT2D, SETD1A and SETD1B (By similarity).
CC {ECO:0000250|UniProtKB:P61964, ECO:0000250|UniProtKB:P61965}.
CC -!- SUBUNIT: Interacts with PAXBP1; the interaction is direct and links a
CC WDR5-containing histone methyltransferase complex to PAX7 and PAX3 (By
CC similarity). Interacts with HCFC1 (By similarity). Component of the
CC ATAC complex, a complex with histone acetyltransferase activity on
CC histones H3 and H4 (By similarity). Component of the SET1 complex, at
CC least composed of the catalytic subunit (SETD1A or SETD1B), WDR5,
CC WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30 (By similarity).
CC Core component of several methyltransferase-containing complexes
CC including MLL1/MLL, MLL2/3 (also named ASCOM complex) and MLL4/WBP7 (By
CC similarity). Each complex is at least composed of ASH2L, RBBP5, WDR5,
CC DPY30, one or more specific histone methyltransferases (KMT2A/MLL1,
CC KMT2D/MLL2, KMT2C/MLL3 and KMT2B/MLL4), and the facultative components
CC PAGR1, BAP18, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1,
CC LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1,
CC PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4,
CC TAF6, TAF7, TAF9, TEX10 and alpha- and beta-tubulin (By similarity).
CC Component of the NSL complex at least composed of MOF/KAT8, KANSL1,
CC KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1 (By similarity).
CC Interacts with KMT2A/MLL1 (via WIN motif) and RBBP5; the interaction is
CC direct (By similarity). Component ofthe ADA2A-containing complex
CC (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5,
CC YEATS2, CCDC101 and DR1 (By similarity). In the complex, it probably
CC interacts directly with KAT2A, MBIP and KAT14 (By similarity).
CC Interacts with histone H3 (By similarity). Interacts with SETD1A (via
CC WIN motif) (By similarity). Component of a histone methylation complex
CC composed of at least ZNF335, RBBP5, ASH2L and WDR5; the complex may
CC have histone H3-specific methyltransferase activity, however does not
CC have specificity for 'Lys-4' of histone H3 (By similarity). Interacts
CC with ZNF335 (By similarity). Components of this complex may associate
CC with components of the ZNF335-CCAR2-EMSY nuclear receptor-mediated
CC transcription complex to form a complex at least composed of ZNF335,
CC HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5 (By similarity).
CC Interacts with PER1 (PubMed:15860628). Interacts with KMT2B (via WIN
CC motif), KMT2C (via WIN motif), KMT2D (via WIN motif) and SETD1B (via
CC WIN motif) (By similarity). {ECO:0000250|UniProtKB:P61964,
CC ECO:0000269|PubMed:15860628}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat WDR5/wds family. {ECO:0000305}.
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DR EMBL; BC100156; AAI00157.1; -; mRNA.
DR RefSeq; NP_001034123.1; NM_001039034.1.
DR RefSeq; XP_006233893.1; XM_006233831.2.
DR PDB; 4QQE; X-ray; 1.80 A; A=24-334.
DR PDBsum; 4QQE; -.
DR AlphaFoldDB; Q498M4; -.
DR SMR; Q498M4; -.
DR CORUM; Q498M4; -.
DR IntAct; Q498M4; 1.
DR STRING; 10116.ENSRNOP00000011556; -.
DR iPTMnet; Q498M4; -.
DR PhosphoSitePlus; Q498M4; -.
DR jPOST; Q498M4; -.
DR PaxDb; 10116-ENSRNOP00000011556; -.
DR Ensembl; ENSRNOT00055010160; ENSRNOP00055007850; ENSRNOG00055006265.
DR Ensembl; ENSRNOT00060045554; ENSRNOP00060037820; ENSRNOG00060026298.
DR Ensembl; ENSRNOT00065035363; ENSRNOP00065028473; ENSRNOG00065020830.
DR GeneID; 362093; -.
DR KEGG; rno:362093; -.
DR UCSC; RGD:1305159; rat.
DR AGR; RGD:1305159; -.
DR CTD; 11091; -.
DR RGD; 1305159; Wdr5.
DR VEuPathDB; HostDB:ENSRNOG00000008212; -.
DR eggNOG; KOG0266; Eukaryota.
DR HOGENOM; CLU_000288_57_1_1; -.
DR InParanoid; Q498M4; -.
DR OrthoDB; 989707at2759; -.
DR PhylomeDB; Q498M4; -.
DR TreeFam; TF314125; -.
DR Reactome; R-RNO-3214841; PKMTs methylate histone lysines.
DR Reactome; R-RNO-3214847; HATs acetylate histones.
DR Reactome; R-RNO-3214858; RMTs methylate histone arginines.
DR Reactome; R-RNO-8936459; RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function.
DR Reactome; R-RNO-8951664; Neddylation.
DR Reactome; R-RNO-9772755; Formation of WDR5-containing histone-modifying complexes.
DR PRO; PR:Q498M4; -.
DR Proteomes; UP000002494; Chromosome 3.
DR Bgee; ENSRNOG00000008212; Expressed in thymus and 20 other cell types or tissues.
DR Genevisible; Q498M4; RN.
DR GO; GO:0140672; C:ATAC complex; ISO:RGD.
DR GO; GO:0000123; C:histone acetyltransferase complex; ISS:UniProtKB.
DR GO; GO:0035097; C:histone methyltransferase complex; ISS:UniProtKB.
DR GO; GO:0071339; C:MLL1 complex; ISS:UniProtKB.
DR GO; GO:0044665; C:MLL1/2 complex; ISO:RGD.
DR GO; GO:0044666; C:MLL3/4 complex; ISO:RGD.
DR GO; GO:0044545; C:NSL complex; ISO:RGD.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048188; C:Set1C/COMPASS complex; ISS:UniProtKB.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0042800; F:histone H3K4 methyltransferase activity; ISO:RGD.
DR GO; GO:0035064; F:methylated histone binding; ISS:UniProtKB.
DR GO; GO:0006094; P:gluconeogenesis; ISO:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0031175; P:neuron projection development; IDA:RGD.
DR GO; GO:0045722; P:positive regulation of gluconeogenesis; ISO:RGD.
DR GO; GO:0051571; P:positive regulation of histone H3-K4 methylation; ISO:RGD.
DR GO; GO:0051726; P:regulation of cell cycle; ISO:RGD.
DR GO; GO:0051302; P:regulation of cell division; ISO:RGD.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; ISO:RGD.
DR GO; GO:0045995; P:regulation of embryonic development; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0090043; P:regulation of tubulin deacetylation; ISO:RGD.
DR GO; GO:0001501; P:skeletal system development; ISO:RGD.
DR GO; GO:0045815; P:transcription initiation-coupled chromatin remodeling; ISS:UniProtKB.
DR CDD; cd00200; WD40; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR19879:SF1; CANNONBALL-RELATED; 1.
DR PANTHER; PTHR19879; TRANSCRIPTION INITIATION FACTOR TFIID; 1.
DR Pfam; PF00400; WD40; 7.
DR PIRSF; PIRSF002394; GN-bd_beta; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 4.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Chromatin regulator; Isopeptide bond; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation; WD repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P61964"
FT CHAIN 2..334
FT /note="WD repeat-containing protein 5"
FT /id="PRO_0000278192"
FT REPEAT 43..82
FT /note="WD 1"
FT REPEAT 85..126
FT /note="WD 2"
FT REPEAT 128..168
FT /note="WD 3"
FT REPEAT 169..208
FT /note="WD 4"
FT REPEAT 212..253
FT /note="WD 5"
FT REPEAT 256..296
FT /note="WD 6"
FT REPEAT 299..333
FT /note="WD 7"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 107
FT /note="Important for interaction with histone H3"
FT /evidence="ECO:0000250"
FT SITE 133
FT /note="Important for interaction with histone H3"
FT /evidence="ECO:0000250"
FT SITE 263
FT /note="Important for interaction with histone H3"
FT /evidence="ECO:0000250"
FT SITE 322
FT /note="Important for interaction with histone H3"
FT /evidence="ECO:0000250"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P61964"
FT MOD_RES 112
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P61964"
FT CROSSLNK 7
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:P61964"
FT CROSSLNK 27
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:P61964"
FT CROSSLNK 46
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:P61964"
FT STRAND 36..41
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 48..53
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 57..64
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 67..73
FT /evidence="ECO:0007829|PDB:4QQE"
FT TURN 74..76
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 79..84
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 90..95
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 99..106
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 111..115
FT /evidence="ECO:0007829|PDB:4QQE"
FT TURN 116..118
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 121..125
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 132..137
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 139..148
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 153..157
FT /evidence="ECO:0007829|PDB:4QQE"
FT TURN 158..160
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 163..167
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 174..179
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 183..190
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 195..199
FT /evidence="ECO:0007829|PDB:4QQE"
FT TURN 200..202
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 205..209
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 217..222
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 226..233
FT /evidence="ECO:0007829|PDB:4QQE"
FT TURN 234..236
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 237..242
FT /evidence="ECO:0007829|PDB:4QQE"
FT TURN 243..246
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 247..252
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 258..260
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 264..267
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 269..271
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 273..276
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 283..287
FT /evidence="ECO:0007829|PDB:4QQE"
FT TURN 288..290
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 293..297
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 304..309
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 311..320
FT /evidence="ECO:0007829|PDB:4QQE"
FT TURN 322..324
FT /evidence="ECO:0007829|PDB:4QQE"
FT STRAND 327..331
FT /evidence="ECO:0007829|PDB:4QQE"
SQ SEQUENCE 334 AA; 36588 MW; 4BF30914A2250286 CRC64;
MATEEKKPET EAARAQPTPS SSATQSKPTP VKPNYALKFT LAGHTKAVSS VKFSPNGEWL
ASSSADKLIK IWGAYDGKFE KTISGHKLGI SDVAWSSDSN LLVSASDDKT LKIWDVSSGK
CLKTLKGHSN YVFCCNFNPQ SNLIVSGSFD ESVRIWDVKT GKCLKTLPAH SDPVSAVHFN
RDGSLIVSSS YDGLCRIWDT ASGQCLKTLI DDDNPPVSFV KFSPNGKYIL AATLDNTLKL
WDYSKGKCLK TYTGHKNEKY CIFANFSVTG GKWIVSGSED NLVYIWNLQT KEIVQKLQGH
TDVVISTACH PTENIIASAA LENDKTIKLW KSDC
//
