GenomeNet

Database: UniProt
Entry: Q4E162
LinkDB: Q4E162
Original site: Q4E162 
ID   IF4A_TRYCC              Reviewed;         404 AA.
AC   Q4E162;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   16-OCT-2019, entry version 64.
DE   RecName: Full=Probable eukaryotic initiation factor 4A;
DE            Short=eIF-4A;
DE            EC=3.6.4.13;
DE   AltName: Full=ATP-dependent RNA helicase eIF4A;
GN   ORFNames=Tc00.1047053511585.190;
OS   Trypanosoma cruzi (strain CL Brener).
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma;
OC   Schizotrypanum.
OX   NCBI_TaxID=353153;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CL Brener;
RX   PubMed=16020725; DOI=10.1126/science.1112631;
RA   El-Sayed N.M.A., Myler P.J., Bartholomeu D.C., Nilsson D.,
RA   Aggarwal G., Tran A.-N., Ghedin E., Worthey E.A., Delcher A.L.,
RA   Blandin G., Westenberger S.J., Caler E., Cerqueira G.C., Branche C.,
RA   Haas B., Anupama A., Arner E., Aslund L., Attipoe P., Bontempi E.,
RA   Bringaud F., Burton P., Cadag E., Campbell D.A., Carrington M.,
RA   Crabtree J., Darban H., da Silveira J.F., de Jong P., Edwards K.,
RA   Englund P.T., Fazelina G., Feldblyum T., Ferella M., Frasch A.C.,
RA   Gull K., Horn D., Hou L., Huang Y., Kindlund E., Klingbeil M.,
RA   Kluge S., Koo H., Lacerda D., Levin M.J., Lorenzi H., Louie T.,
RA   Machado C.R., McCulloch R., McKenna A., Mizuno Y., Mottram J.C.,
RA   Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M., Pentony M.,
RA   Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L.,
RA   Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J.,
RA   Simpson A.J., Sisk E., Tammi M.T., Tarleton R., Teixeira S.,
RA   Van Aken S., Vogt C., Ward P.N., Wickstead B., Wortman J., White O.,
RA   Fraser C.M., Stuart K.D., Andersson B.;
RT   "The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas
RT   disease.";
RL   Science 309:409-415(2005).
CC   -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the
CC       eIF4F complex involved in cap recognition and is required for mRNA
CC       binding to ribosome. In the current model of translation
CC       initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR
CC       of mRNAs which is necessary to allow efficient binding of the
CC       small ribosomal subunit, and subsequent scanning for the initiator
CC       codon (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of
CC       which varies with external and internal environmental conditions.
CC       It is composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A
CC       subfamily. {ECO:0000305}.
DR   EMBL; AAHK01000053; EAN98527.1; -; Genomic_DNA.
DR   RefSeq; XP_820378.1; XM_815285.1.
DR   SMR; Q4E162; -.
DR   STRING; 5693.XP_820378.1; -.
DR   PaxDb; Q4E162; -.
DR   EnsemblProtists; EAN98527; EAN98527; Tc00.1047053511585.190.
DR   GeneDB; TcCLB.511585.190:pep; -.
DR   GeneID; 3553014; -.
DR   KEGG; tcr:511585.190; -.
DR   eggNOG; KOG0327; Eukaryota.
DR   eggNOG; COG0513; LUCA.
DR   KO; K03257; -.
DR   OMA; AEYDNRD; -.
DR   OrthoDB; 726081at2759; -.
DR   Proteomes; UP000002296; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome; RNA-binding.
FT   CHAIN         1    404       Probable eukaryotic initiation factor 4A.
FT                                /FTId=PRO_0000291645.
FT   DOMAIN       57    231       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      242    402       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      70     77       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF        26     54       Q motif.
FT   MOTIF       179    182       DEAD box.
SQ   SEQUENCE   404 AA;  45504 MW;  F0D417A63851E3B5 CRC64;
     MAQQGKVEPQ DQDSFLDDQP GIRPIPSFDD MPLHQNLLRG IYSHGFEKPS SIQQRAIVPF
     TRGGDIIAQA QSGTGKTGAF SIGLLQRLDF RHNVLQGLVL SPTRELALQT AEVITRIGEF
     LAEGNSSFCA TFVGGTRVQD DYRKLQAGSI VAVGTPGRVV DVTKRGAMRT EHLRVLVLDE
     ADEMLSQGFA EQIYEIFRYL PKEIQVALFS ATMPDDVLEL TKKFMRDPTR ILVKRESLTL
     EGIKQYFIAV EEEHKLDTLM DLYETVSIAQ SVIFANTRRK VDWLAQQLNQ SNHTVSCMHS
     EMPKQDREKV MSTFRNGSSR VLVTTDLVAR GIDVHHVNIV INFDLPTNKE SYLHRIGRGG
     RYGRKGVAIN FVTQKDVEVL REIESHYHTQ IDELPVDFAA YLGE
//
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