ID Q4K8I0_PSEF5 Unreviewed; 200 AA.
AC Q4K8I0;
DT 02-AUG-2005, integrated into UniProtKB/TrEMBL.
DT 02-AUG-2005, sequence version 1.
DT 27-MAR-2024, entry version 113.
DE SubName: Full=Glutathione S-transferase {ECO:0000313|EMBL:AAY93616.1};
DE EC=2.5.1.18 {ECO:0000313|EMBL:AAY93616.1};
GN OrderedLocusNames=PFL_4361 {ECO:0000313|EMBL:AAY93616.1};
OS Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=220664 {ECO:0000313|EMBL:AAY93616.1, ECO:0000313|Proteomes:UP000008540};
RN [1] {ECO:0000313|EMBL:AAY93616.1, ECO:0000313|Proteomes:UP000008540}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5
RC {ECO:0000313|Proteomes:UP000008540};
RX PubMed=15980861; DOI=10.1038/nbt1110;
RA Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.,
RA Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S.III.,
RA Thomashow L.S., Loper J.E.;
RT "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT Pf-5.";
RL Nat. Biotechnol. 23:873-878(2005).
RN [2] {ECO:0007829|PDB:3M3M}
RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 2-200 IN COMPLEX WITH
RP GLUTATHIONE.
RA Bagaria A., Burley S.K., Swaminathan S.;
RT "Crystal structure of glutathione S-transferase from Pseudomonas
RT fluorescens [Pf-5].";
RL Submitted (MAR-2010) to the PDB data bank.
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DR EMBL; CP000076; AAY93616.1; -; Genomic_DNA.
DR RefSeq; WP_011062631.1; NC_004129.6.
DR PDB; 3M3M; X-ray; 1.75 A; A=2-200.
DR PDBsum; 3M3M; -.
DR AlphaFoldDB; Q4K8I0; -.
DR SMR; Q4K8I0; -.
DR STRING; 220664.PFL_4361; -.
DR DNASU; 3478484; -.
DR GeneID; 57477438; -.
DR KEGG; pfl:PFL_4361; -.
DR PATRIC; fig|220664.5.peg.4468; -.
DR eggNOG; COG0625; Bacteria.
DR HOGENOM; CLU_011226_6_0_6; -.
DR EvolutionaryTrace; Q4K8I0; -.
DR Proteomes; UP000008540; Chromosome.
DR GO; GO:0004364; F:glutathione transferase activity; IEA:UniProtKB-EC.
DR CDD; cd03178; GST_C_Ure2p_like; 1.
DR CDD; cd03056; GST_N_4; 1.
DR Gene3D; 1.20.1050.10; -; 1.
DR Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR004046; GST_C.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR44051; GLUTATHIONE S-TRANSFERASE-RELATED; 1.
DR PANTHER; PTHR44051:SF2; HYPOTHETICAL GLUTATHIONE S-TRANSFERASE LIKE PROTEIN; 1.
DR Pfam; PF00043; GST_C; 1.
DR Pfam; PF13409; GST_N_2; 1.
DR SFLD; SFLDG01151; Main.2:_Nu-like; 1.
DR SFLD; SFLDG00358; Main_(cytGST); 1.
DR SUPFAM; SSF47616; GST C-terminal domain-like; 1.
DR SUPFAM; SSF52833; Thioredoxin-like; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS50404; GST_NTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:3M3M};
KW Transferase {ECO:0000313|EMBL:AAY93616.1}.
FT DOMAIN 1..82
FT /note="GST N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50404"
FT DOMAIN 84..200
FT /note="GST C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50405"
FT BINDING 39
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0007829|PDB:3M3M"
FT BINDING 52
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0007829|PDB:3M3M"
FT BINDING 53
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0007829|PDB:3M3M"
FT BINDING 67
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0007829|PDB:3M3M"
FT BINDING 165
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0007829|PDB:3M3M"
SQ SEQUENCE 200 AA; 23257 MW; 2C4C398AEE71B47B CRC64;
MYKVYGDYRS GNCYKIKLML NLLGLPYEWQ AVDILGGDTQ TEAFLAKNPN GKIPVLELED
GTCLWESNAI LNFLADGSQF LPSEPRLRTQ VLQWQFFEQY SHEPYIAVAR FIQLYEGLPE
ERREEYLKLH KRGYKALDVM EKQLSRTPYL VGEHYSIADI ALYAYTHVAD EGGFDLSRYP
GIQAWMQRVQ SHPRHVPMLD
//
