ID Q4QC77_LEIMA Unreviewed; 786 AA.
AC Q4QC77;
DT 19-JUL-2005, integrated into UniProtKB/TrEMBL.
DT 19-JUL-2005, sequence version 1.
DT 27-MAR-2024, entry version 93.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=LMJF_21_1190 {ECO:0000313|EMBL:CAJ04624.1};
OS Leishmania major.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania.
OX NCBI_TaxID=5664 {ECO:0000313|EMBL:CAJ04624.1, ECO:0000313|Proteomes:UP000000542};
RN [1] {ECO:0000313|EMBL:CAJ04624.1, ECO:0000313|Proteomes:UP000000542}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MHOM/IL/81/Friedlin {ECO:0000313|Proteomes:UP000000542};
RX PubMed=16020728; DOI=10.1126/science.1112680;
RA Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G.,
RA Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A.,
RA Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M.,
RA Bianchettin G., Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E.,
RA Ciarloni L., Clayton C., Coulson R.M., Cronin A., Cruz A.K., Davies R.M.,
RA De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N.,
RA Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A.,
RA Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S.,
RA Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M.,
RA Masuy D., Matthews K., Michaeli S., Mottram J.C., Muller-Auer S.,
RA Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M.,
RA Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E.,
RA Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C.,
RA Rutter S., Saunders D., Schafer M., Schein J., Schwartz D.C., Seeger K.,
RA Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V.,
RA Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J.,
RA Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D.,
RA Barrell B., Myler P.J.;
RT "The genome of the kinetoplastid parasite, Leishmania major.";
RL Science 309:436-442(2005).
RN [2] {ECO:0000313|EMBL:CAJ04624.1, ECO:0000313|Proteomes:UP000000542}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MHOM/IL/81/Friedlin {ECO:0000313|Proteomes:UP000000542};
RX PubMed=22038252; DOI=10.1101/gr.122945.111;
RA Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A.,
RA Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D.,
RA Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F.,
RA Hertz-Fowler C., Mottram J.C.;
RT "Chromosome and gene copy number variation allow major structural change
RT between species and strains of Leishmania.";
RL Genome Res. 21:2129-2142(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|RuleBase:RU363044};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SIMILARITY: Belongs to the helicase family. PIF1 subfamily.
CC {ECO:0000256|ARBA:ARBA00009781}.
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DR EMBL; FR796417; CAJ04624.1; -; Genomic_DNA.
DR RefSeq; XP_001683071.1; XM_001683019.1.
DR AlphaFoldDB; Q4QC77; -.
DR STRING; 5664.Q4QC77; -.
DR EnsemblProtists; CAJ04624; CAJ04624; LMJF_21_1190.
DR GeneID; 5651678; -.
DR KEGG; lma:LMJF_21_1190; -.
DR VEuPathDB; TriTrypDB:LmjF.21.1190; -.
DR VEuPathDB; TriTrypDB:LMJFC_210021300; -.
DR VEuPathDB; TriTrypDB:LMJLV39_210020000; -.
DR VEuPathDB; TriTrypDB:LMJSD75_210020200; -.
DR eggNOG; KOG0987; Eukaryota.
DR HOGENOM; CLU_356976_0_0_1; -.
DR InParanoid; Q4QC77; -.
DR OMA; KTEWLLH; -.
DR Proteomes; UP000000542; Chromosome 21.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0031981; C:nuclear lumen; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISO:GeneDB.
DR GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR GO; GO:0043139; F:5'-3' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; ISO:GeneDB.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0000287; F:magnesium ion binding; ISO:GeneDB.
DR GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18037; DEXSc_Pif1_like; 1.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR47642; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR47642:SF2; PHD-TYPE DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF05970; PIF1; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000000542}.
FT REGION 126..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 208..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 718..786
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..141
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 786 AA; 84150 MW; C774BA89A9AD8698 CRC64;
MAAVAIRVPK PKMTMSAVQG KISVFAEDGE RIGQWGGTEC FLSRQGGLGP CLVVRSSRHK
KHEGTFFQLV RLQRVVSTGV AQGRLTVMVP HEKRQCSVFI DTTEDLDELR MMAGVLQDKA
RWKDMERNVA SRSRRGPQRG GKGDGGGRGS GELRDPGLAQ LSGSGVDSDD DYDASGEDTR
KTHHDNDTVA VSAAQGDSFA AHSMVESLTH MTSSQSSVPQ RSTVVAGGGS SSSSGPTTSL
TKAQQQRLKW TSEQQRAVQL VRAGHNVFVS GAAGTGKTEW LLHVLQHVLP RTRQHRGAKS
KADLGAEGED QEQVVETGRV AVTAATGIAA RLIGGKTVHA FSGIGRGEGD PDAILQRVQS
NPDVVRAWQR CEVLVIDEIS MLSSHTFALL DRVARVLRAP LAPPVSSSQR RQRTSNAALP
FGGIQLLVVG DFLQLPPVSR GAGEEVQPAF MAAVWRACAF QQLVFTKDYR HAEDPRFAEC
CAAVRRGECT PLVRQVLEAC LGRKLEERFG VEATTLLARR KDVDRYNAHR LQQLESMQFQ
RYASEDYAAV PGADIDAEVS LPTVLTLKVG AQVVLLASLP NEPSLANGNL GLVGGFVAQA
HGPALPLVRF STGVEAVVPA VTMEVHGRDG RLSLSRRQVP LQLAWALTVH RVQGMTLPMV
RLALDKSFFE AGQAYVALSR VRKAEDLSLT ALDLDVVAAC VSVEARDFYG LGTVTSAPSS
QPVITRAGSS PPLEPALSRE RDTACGKGSL RTDGVSGSGV PLSLADIEGG GSRVTATRKR
AKTEDG
//
