ID Q4QCK3_LEIMA Unreviewed; 812 AA.
AC Q4QCK3;
DT 19-JUL-2005, integrated into UniProtKB/TrEMBL.
DT 19-JUL-2005, sequence version 1.
DT 27-MAR-2024, entry version 107.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
GN ORFNames=LMJF_21_0125 {ECO:0000313|EMBL:CAJ03761.1};
OS Leishmania major.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania.
OX NCBI_TaxID=5664 {ECO:0000313|EMBL:CAJ03761.1, ECO:0000313|Proteomes:UP000000542};
RN [1] {ECO:0000313|EMBL:CAJ03761.1, ECO:0000313|Proteomes:UP000000542}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MHOM/IL/81/Friedlin {ECO:0000313|Proteomes:UP000000542};
RX PubMed=16020728; DOI=10.1126/science.1112680;
RA Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G.,
RA Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A.,
RA Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M.,
RA Bianchettin G., Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E.,
RA Ciarloni L., Clayton C., Coulson R.M., Cronin A., Cruz A.K., Davies R.M.,
RA De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N.,
RA Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A.,
RA Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S.,
RA Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M.,
RA Masuy D., Matthews K., Michaeli S., Mottram J.C., Muller-Auer S.,
RA Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M.,
RA Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E.,
RA Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C.,
RA Rutter S., Saunders D., Schafer M., Schein J., Schwartz D.C., Seeger K.,
RA Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V.,
RA Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J.,
RA Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D.,
RA Barrell B., Myler P.J.;
RT "The genome of the kinetoplastid parasite, Leishmania major.";
RL Science 309:436-442(2005).
RN [2] {ECO:0000313|EMBL:CAJ03761.1, ECO:0000313|Proteomes:UP000000542}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MHOM/IL/81/Friedlin {ECO:0000313|Proteomes:UP000000542};
RX PubMed=22038252; DOI=10.1101/gr.122945.111;
RA Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A.,
RA Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D.,
RA Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F.,
RA Hertz-Fowler C., Mottram J.C.;
RT "Chromosome and gene copy number variation allow major structural change
RT between species and strains of Leishmania.";
RL Genome Res. 21:2129-2142(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
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DR EMBL; FR796417; CAJ03761.1; -; Genomic_DNA.
DR RefSeq; XP_001682945.1; XM_001682893.1.
DR AlphaFoldDB; Q4QCK3; -.
DR STRING; 5664.Q4QCK3; -.
DR EnsemblProtists; CAJ03761; CAJ03761; LMJF_21_0125.
DR GeneID; 5651546; -.
DR KEGG; lma:LMJF_21_0125; -.
DR VEuPathDB; TriTrypDB:LmjF.21.0125; -.
DR VEuPathDB; TriTrypDB:LMJFC_210007100; -.
DR VEuPathDB; TriTrypDB:LMJLV39_210006400; -.
DR VEuPathDB; TriTrypDB:LMJSD75_210006400; -.
DR eggNOG; KOG1956; Eukaryota.
DR HOGENOM; CLU_349701_0_0_1; -.
DR InParanoid; Q4QCK3; -.
DR OMA; ARHAMDR; -.
DR Proteomes; UP000000542; Chromosome 21.
DR GO; GO:0005737; C:cytoplasm; ISO:GeneDB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd01028; TOPRIM_TopoIA; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR42785:SF1; DNA TOPOISOMERASE; 1.
DR PANTHER; PTHR42785; DNA TOPOISOMERASE, TYPE IA, CORE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:CAJ03761.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000542};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 10..166
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT REGION 385..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 758..812
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 760..788
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 812 AA; 89497 MW; 5289D2E62A0E1A72 CRC64;
MLRRSVRALE KLVIVESPNK VIKVEGLLSD PKVIPDWSFN NSKLRAISTG AEKAIAMATT
GHFMSLKELT WSPQSSSPAS GVTAADFPAN GLLVSFSLEW ELIPGRRIQD TVSHYIEEKA
DNLTEIIVAT DPDREGELIA VHAQNLIRRM FPQLNAPFTR AYMHSITADG IRRAMEERHE
AFDYNLANAA EARHAMDRIF GFLGSSVVRY ANPQMRSIGR VQTPALILIN DREDKIKSYL
ESHASTFEIQ VVCYFTSKHH QRFSQVVKVT PARKGGAAPV DWSDKATVKQ RCEQWALSSA
THFSVSPGAK PQETVTPPPK PFTMATLIAR ANRQLHYSSD MVSSCLQDLF QMGYITYPRT
DSTRIDESIL PSIYAAVRRE HGKRLLQEQD GPSKAGVRRS TAQKEAADAN VEDAHEAIRP
TNIDTTVEEL GAVSAPMKHI YDLVRRNTMA VHMIPMKTER IVVLVACKAA NGEEVEFELQ
GKHVVEPGWS AAFHGSKGTA TPVTETDQDV EAVEDGGVVV PSISDDEFKA IMDIAAQRGG
GTGVQKGSMQ LESAQVAENR PSPPLPFSEG GLIEQLKNNG VGRPSTYPMI VKTLLARNYI
TVNKGRCETT PVGRMLVDTS RATFPSIVDI GFTSAFEKKL DRIAKPRSTK ELALPPNVSD
ADYVLSSFIS NFLNYVTEAT KAQRVAITTR SLALQQEKRA ASNTPMSPTD FQANLEKETK
RVLTQVPDLV DNMKSYRTFT ALQNSLNDYL RRNFPPSAAA MAPASSSGTA RSYGSSSSSS
SSTKKKESYG ATYRVDKKTP RHFRAKLKKA KK
//
