GenomeNet

Database: UniProt
Entry: Q4WA22
LinkDB: Q4WA22
Original site: Q4WA22 
ID   DCL2_ASPFU              Reviewed;        1388 AA.
AC   Q4WA22;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 2.
DT   31-JUL-2019, entry version 88.
DE   RecName: Full=Dicer-like protein 2;
DE   Includes:
DE     RecName: Full=Endoribonuclease dcl2;
DE              EC=3.1.26.-;
DE   Includes:
DE     RecName: Full=ATP-dependent helicase dcl2;
DE              EC=3.6.4.-;
GN   Name=dcl2; ORFNames=AFUA_4G02930;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S.,
RA   Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W.,
RA   Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S.,
RA   Farman M.L., Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R.,
RA   Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A.,
RA   Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J.,
RA   Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J.,
RA   Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S.,
RA   Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A.,
RA   Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M.,
RA   Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I.,
RA   Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A.,
RA   Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M.,
RA   Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S.,
RA   Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J.,
RA   White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K.,
RA   Machida M., Hall N., Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
CC   -!- FUNCTION: Dicer-like endonuclease involved in cleaving double-
CC       stranded RNA in the RNA interference (RNAi) pathway. Produces 21
CC       to 25 bp dsRNAs (siRNAs) which target the selective destruction of
CC       homologous RNAs leading to sequence-specific suppression of gene
CC       expression, called post-transcriptional gene silencing (PTGS).
CC       Part of a broad host defense response against viral infection and
CC       transposons (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the helicase family. Dicer subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00657}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAL84441.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AAHF01000016; EAL84441.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_746479.1; XM_741386.1.
DR   STRING; 746128.CADAFUBP00009739; -.
DR   PRIDE; Q4WA22; -.
DR   GeneID; 3503874; -.
DR   KEGG; afm:AFUA_4G02930; -.
DR   EuPathDB; FungiDB:Afu4g02930; -.
DR   HOGENOM; HOG000048683; -.
DR   InParanoid; Q4WA22; -.
DR   OrthoDB; 1337630at2759; -.
DR   Proteomes; UP000002530; Chromosome 4.
DR   Proteomes; UP000002530; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0016442; C:RISC complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004525; F:ribonuclease III activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0030422; P:production of siRNA involved in RNA interference; IBA:GO_Central.
DR   GO; GO:0050688; P:regulation of defense response to virus; IEA:UniProtKB-KW.
DR   CDD; cd00593; RIBOc; 2.
DR   Gene3D; 1.10.1520.10; -; 2.
DR   Gene3D; 3.30.160.380; -; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR038248; Dicer_dimer_sf.
DR   InterPro; IPR005034; Dicer_dimerisation_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF03368; Dicer_dimer; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF00636; Ribonuclease_3; 2.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00535; RIBOc; 2.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF69065; SSF69065; 2.
DR   PROSITE; PS51327; DICER_DSRBF; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS00517; RNASE_3_1; 1.
DR   PROSITE; PS50142; RNASE_3_2; 2.
PE   3: Inferred from homology;
KW   Antiviral defense; Antiviral protein; ATP-binding; Complete proteome;
KW   Helicase; Hydrolase; Magnesium; Manganese; Metal-binding;
KW   Nucleotide-binding; Reference proteome; Repeat; RNA-binding.
FT   CHAIN         1   1388       Dicer-like protein 2.
FT                                /FTId=PRO_0000306786.
FT   DOMAIN       23    203       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      368    537       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   DOMAIN      564    658       Dicer dsRNA-binding fold.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00657}.
FT   DOMAIN      906   1059       RNase III 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00177}.
FT   DOMAIN     1098   1281       RNase III 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00177}.
FT   NP_BIND      36     43       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       144    147       DEAH box.
FT   METAL      1137   1137       Magnesium or manganese. {ECO:0000250}.
FT   METAL      1267   1267       Magnesium or manganese. {ECO:0000250}.
FT   METAL      1270   1270       Magnesium or manganese. {ECO:0000250}.
FT   SITE       1263   1263       Important for activity. {ECO:0000250}.
SQ   SEQUENCE   1388 AA;  156878 MW;  1710B5AFB4B420A3 CRC64;
     MASTVSTCQD ASPYQPRNYQ LEMLEASMKE NIIVAMDTGS GKTHIAVLRI KAELDICSPD
     KVVWFLAPTV ALCIQQHEVI ASNLPAVRTR TLTGLDKVEL WTDQSIWDAV LNGYRVIVST
     HAVLADALSH GFVKMSQLAL LIFDEAHHCT RRHAANKIMQ DFYHPTLTKS GPAAVPRIMG
     LTASPVVRSN HQELLMVESN LDAVCRTPRL HRQELLKFTH RPHLQQIWYT PTDPAGFRSA
     SLTLGALYHA WENLDIGDDP YIQRLRKSPL DDRALKKALL TGKTYCREQL RRFVDRSRHI
     FEELGEWAAE YYIYASIKQL RDRVRDSYMS GDWDEAEKAY LVDFLSKIPT SDIHFALNDP
     DGFRISPKFE SLLNFLDSSD QREFSGLIFV KQRVTVSAMT SLLSVHPYTR ERFRCAAYVG
     WSNSSASKDI LGDLLNMQLQ RDTLDDFRSG RKNLIIATDV LEEGIDLSAC SVVVCYDKPP
     NLKSFIQRRG RARRKQSTFA IMFPTDDTSA DVSRWQDLEQ AMIEAYQDDE RQLQSVSALE
     SLDEEVMERL TGDSTSAVLT ADMAMAHLHH FCAVLPPQPY VDMRPVFSFE TNEDGLLKGT
     VILPSCVHPK VRRTEGRRWW RTERAAMKET AFQAYKSLYE FGLVNDHLLP LTKKPELKTH
     DLGSMPSILE TSEQYDPWIE WAYSWSSPDI HQSRIVVKMN EGRGDELCMR LIGPEYLPPL
     SPMTLFWNSS TTFTVTFKAA ERVPLVPLSS VEDMRAITAL YLKATSSRVC SAERDFMALF
     APDLHHTELK GWLDTYEGSD PAMEVYSRGH NPLLMGVVRD HSRYGEPFLF RKWLVSHQNP
     SCSIVELECA PFPHRRNFLR RQTLANSQVD VDEAIPDSAA KNPIVAAEAC TIDRLPFTMA
     IFGLFISAIV EQLEIELIAT RLRDTILRDV SFKSTDHIIT AISTPLAHRL TNYQRYEFLG
     DSILKFSVSC QLFFQHPNWH EGYLSEGRDM IVQNPRLAKA ALDTGLDAYI VTKRLASRKW
     SAPLISEKLG RVPAKRQMST KVLADVVEAL IGAAYVDGGH STAQACIRRF LPEINLHAVD
     TRTAARSVAP ESARHMMNDR LKDHIGYTFE DESLLVEALT HPSCDYDSTT QSYQRLEYLG
     DAVLDMVIVS AIFNHRIQRP QGDMTKIKHA VVNANLLAFL CMEFAISEEK LDVAQTSKDS
     FAVTSSQESV ELWRFMRYRG QGLKAARDAS LARHRALRDE IASSLLQAPH YPWHALSRLN
     ADKFFSDIIE SVLGAIFVDS GGNLAPCEVF VEQIGLMAYL RRILDHGIDV RHPRSVVQQL
     AKTNIQFVLQ RVPTEEGGAS YQCSVQMEQA ELFVVTGCLT AEEAEVTAAV EAIKFLTRDE
     GSTPLNKS
//
DBGET integrated database retrieval system