GenomeNet

Database: UniProt
Entry: Q4WYJ7
LinkDB: Q4WYJ7
Original site: Q4WYJ7 
ID   SPB4_ASPFU              Reviewed;         640 AA.
AC   Q4WYJ7;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 2.
DT   16-OCT-2019, entry version 92.
DE   RecName: Full=ATP-dependent rRNA helicase spb4 {ECO:0000305};
DE            EC=3.6.4.13 {ECO:0000250|UniProtKB:P25808};
GN   Name=spb4 {ECO:0000250|UniProtKB:P25808}; ORFNames=AFUA_3G13280;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S.,
RA   Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W.,
RA   Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S.,
RA   Farman M.L., Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R.,
RA   Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A.,
RA   Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J.,
RA   Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J.,
RA   Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S.,
RA   Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A.,
RA   Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M.,
RA   Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I.,
RA   Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A.,
RA   Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M.,
RA   Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S.,
RA   Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J.,
RA   White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K.,
RA   Machida M., Hall N., Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
CC   -!- FUNCTION: ATP-binding RNA helicase involved in the biogenesis of
CC       60S ribosomal subunits. Binds 90S pre-ribosomal particles and
CC       dissociates from pre-60S ribosomal particles after processing of
CC       27SB pre-rRNA. Required for the normal formation of 18S rRNA
CC       through the processing of pre-rRNAs at sites A0, A1 and A2, and
CC       the normal formation of 25S and 5.8S rRNAs through the processing
CC       of pre-rRNAs at sites C1 and C2. {ECO:0000250|UniProtKB:P25808}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000250|UniProtKB:P25808};
CC   -!- SUBUNIT: Component of pre-60S ribosomal complexes.
CC       {ECO:0000250|UniProtKB:P25808}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:P25808}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX55/SPB4
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAL92256.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AAHF01000002; EAL92256.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_754294.1; XM_749201.1.
DR   SMR; Q4WYJ7; -.
DR   STRING; 746128.CADAFUBP00003514; -.
DR   GeneID; 3511733; -.
DR   KEGG; afm:AFUA_3G13280; -.
DR   EuPathDB; FungiDB:Afu3g13280; -.
DR   HOGENOM; HOG000268803; -.
DR   InParanoid; Q4WYJ7; -.
DR   KO; K14809; -.
DR   OrthoDB; 973872at2759; -.
DR   Proteomes; UP000002530; Chromosome 3.
DR   Proteomes; UP000002530; Unassembled WGS sequence.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR025313; DUF4217.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF13959; DUF4217; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM01178; DUF4217; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Coiled coil; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA processing.
FT   CHAIN         1    640       ATP-dependent rRNA helicase spb4.
FT                                /FTId=PRO_0000232321.
FT   DOMAIN       45    249       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      283    437       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      58     65       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   COILED      521    629       {ECO:0000255}.
FT   MOTIF        14     42       Q motif. {ECO:0000305}.
FT   MOTIF       197    200       DEAD box. {ECO:0000305}.
SQ   SEQUENCE   640 AA;  70950 MW;  4A445E3001D752B2 CRC64;
     MAPKPPSGTS LRAWDAVTPA LSEWVLEAMS SMGFTRMTPV QASAIPLFMA HKDVVVEAVT
     GSGKTLSFLI PVVEKLLRLE EPIKKHHVGA IIISPTRELA SQIYNVLSSL LAFHPPSAAA
     INPSEDDDAP RPKFPSSTLK VVPQLLLGGS TTPAEDLSTF LKRSPNVLVS TPGRLLELLS
     SPHVHCPQSS FEMLVLDEAD RLLDLGFKET LQNILRRLPK QRRTGLFSAS VSEAVDQIVR
     VGLRNPVKVM VKVKGGSGVD DKRTPASLQM TYLTTPPSHK FAALKRIVSS VQPTPLKTIF
     FVSTCSGVDY LSAILPLLLG DDFLLIPLHG KHQANVRQKN FNRFINSHDP AILLTTDVAA
     RGLDIPSVDL VVQIDPPSDP KSFIHRCGRA GRAGRRGLSV VLLHPGREED YVSFLEVRKT
     PVVPFSPSIS FSDADAAAAT ATARKAVLAD RALHDRGQKA FVSWLRSYSK HQASSIFRVS
     DLDWEALGKA WGLLKLPKMP ELKNFTGDKT LGMSLDWDNY AYKDKQREKR RKELLQEAAE
     SGATQSTSNK RRATESVAWS QQAESKNKKL KRREQKKSKQ EKARWEKMTE EEKQKVLETE
     KMVEELRKKN EEERRLRRAV AKAAGAKADG DDEEEFQGFD
//
DBGET integrated database retrieval system