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Database: UniProt
Entry: Q53727
LinkDB: Q53727
Original site: Q53727 
ID   PCRA_STAA8              Reviewed;         730 AA.
AC   Q53727; Q2G1X9;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 3.
DT   10-OCT-2018, entry version 105.
DE   RecName: Full=ATP-dependent DNA helicase PcrA;
DE            EC=3.6.4.12;
GN   Name=pcrA; OrderedLocusNames=SAOUHSC_02123;
OS   Staphylococcus aureus (strain NCTC 8325).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTANT PCRA3.
RX   PubMed=8232203; DOI=10.1007/BF00280216;
RA   Iordanescu S.;
RT   "Characterization of the Staphylococcus aureus chromosomal gene pcrA,
RT   identified by mutations affecting plasmid pT181 replication.";
RL   Mol. Gen. Genet. 241:185-192(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W.,
RA   Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press,
RL   Washington D.C. (2006).
CC   -!- FUNCTION: Essential helicase. May act as a helicase in plasmid
CC       pT181 replication.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA72091.1; Type=Frameshift; Positions=666; Evidence={ECO:0000305};
DR   EMBL; M63176; AAA72091.1; ALT_FRAME; Unassigned_DNA.
DR   EMBL; CP000253; ABD31172.1; -; Genomic_DNA.
DR   PIR; S39923; S39923.
DR   RefSeq; WP_000992921.1; NZ_LS483365.1.
DR   RefSeq; YP_500614.1; NC_007795.1.
DR   ProteinModelPortal; Q53727; -.
DR   SMR; Q53727; -.
DR   STRING; 93061.SAOUHSC_02123; -.
DR   PRIDE; Q53727; -.
DR   EnsemblBacteria; ABD31172; ABD31172; SAOUHSC_02123.
DR   GeneID; 3921194; -.
DR   KEGG; sao:SAOUHSC_02123; -.
DR   PATRIC; fig|93061.5.peg.1926; -.
DR   eggNOG; ENOG4105C4R; Bacteria.
DR   eggNOG; COG0210; LUCA.
DR   HOGENOM; HOG000033016; -.
DR   KO; K03657; -.
DR   OMA; YQDTNRT; -.
DR   BioCyc; SAUR93061:G1G5Y-2010-MONOMER; -.
DR   PRO; PR:Q53727; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR   Gene3D; 1.10.10.160; -; 1.
DR   InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01073; pcrA; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN         1    730       ATP-dependent DNA helicase PcrA.
FT                                /FTId=PRO_0000102061.
FT   DOMAIN        6    285       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00560}.
FT   DOMAIN      286    560       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00617}.
FT   NP_BIND      30     35       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00560}.
FT   BINDING     283    283       ATP. {ECO:0000250}.
FT   MUTAGEN      61     61       T->I: In pcrA3; affects plasmid pT181
FT                                replication.
FT   CONFLICT    666    667       Missing (in Ref. 1). {ECO:0000305}.
SQ   SEQUENCE   730 AA;  84074 MW;  3C7D6A9501BC3251 CRC64;
     MNALLNHMNT EQSEAVKTTE GPLLIMAGAG SGKTRVLTHR IAYLLDEKDV SPYNVLAITF
     TNKAAREMKE RVQKLVGDQA EVIWMSTFHS MCVRILRRDA DRIGIERNFT IIDPTDQKSV
     IKDVLKNENI DSKKFEPRMF IGAISNLKNE LKTPADAQKE ATDYHSQMVA TVYSGYQRQL
     SRNEALDFDD LIMTTINLFE RVPEVLEYYQ NKFQYIHVDE YQDTNKAQYT LVKLLASKFK
     NLCVVGDSDQ SIYGWRGADI QNILSFEKDY PEANTIFLEQ NYRSTKTILN AANEVIKNNS
     ERKPKGLWTA NTNGEKIHYY EAMTERDEAE FVIREIMKHQ RNGKKYQDMA ILYRTNAQSR
     VLEETFMKSN MPYTMVGGQK FYDRKEIKDL LSYLRIIANS NDDISLQRII NVPKRGVGPS
     SVEKVQNYAL QNNISMFDAL GEADFIGLSK KVTQECLNFY ELIQSLIKEQ EFLEIHEIVD
     EVLQKSGYRE MLERENTLES RSRLENIDEF MSVPKDYEEN TPLEEQSLIN FLTDLSLVAD
     IDEADTENGV TLMTMHSAKG LEFPIVFIMG MEESLFPHIR AIKSEDDHEM QEERRICYVA
     ITRAEEVLYI THATSRMLFG RPQSNMPSRF LKEIPESLLE NHSSGKRQTI QPKAKPFAKR
     GFSQRTTSTK KQVLSSDWNV GDKVMHKAWG EGMVSNVNEK NGSIELDIIF KSQGPKRLLA
     QFAPIEKKED
//
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