ID ATR1_DICDI Reviewed; 3157 AA.
AC Q54ER4;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 27-MAR-2024, entry version 116.
DE RecName: Full=Probable serine/threonine-protein kinase atr1;
DE EC=2.7.11.1;
DE AltName: Full=Ataxia telangiectasia and rad3 related protein 1;
GN Name=atr1; ORFNames=DDB_G0291380;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16596165; DOI=10.1371/journal.pgen.0020038;
RA Goldberg J.M., Manning G., Liu A., Fey P., Pilcher K.E., Xu Y., Smith J.L.;
RT "The dictyostelium kinome -- analysis of the protein kinases from a simple
RT model organism.";
RL PLoS Genet. 2:E38-E38(2006).
CC -!- FUNCTION: Serine/threonine protein kinase which activates checkpoint
CC signaling upon genotoxic stresses such as ionizing radiation (IR),
CC ultraviolet light (UV), or DNA replication stalling, thereby acting as
CC a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily.
CC {ECO:0000305}.
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DR EMBL; AAFI02000177; EAL61674.1; -; Genomic_DNA.
DR RefSeq; XP_635176.1; XM_630084.1.
DR STRING; 44689.Q54ER4; -.
DR PaxDb; 44689-DDB0229332; -.
DR EnsemblProtists; EAL61674; EAL61674; DDB_G0291380.
DR GeneID; 8628122; -.
DR KEGG; ddi:DDB_G0291380; -.
DR dictyBase; DDB_G0291380; atr1.
DR eggNOG; KOG0890; Eukaryota.
DR HOGENOM; CLU_225616_0_0_1; -.
DR InParanoid; Q54ER4; -.
DR OMA; FMACRGI; -.
DR PhylomeDB; Q54ER4; -.
DR PRO; PR:Q54ER4; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; ISS:dictyBase.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0000077; P:DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; ISS:dictyBase.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR CDD; cd00892; PIKKc_ATR; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR012993; UME.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF72; SERINE-PROTEIN KINASE ATM; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF08064; UME; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SMART; SM00802; UME; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding; Coiled coil; DNA damage; DNA repair; Kinase;
KW Nucleotide-binding; Nucleus; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..3157
FT /note="Probable serine/threonine-protein kinase atr1"
FT /id="PRO_0000376001"
FT DOMAIN 2042..2714
FT /note="FAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00534"
FT DOMAIN 2817..3121
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT DOMAIN 3125..3157
FT /note="FATC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00534,
FT ECO:0000255|PROSITE-ProRule:PRU00535"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 55..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 601..623
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 829..857
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 869..897
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1283..1319
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2823..2829
FT /note="G-loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 2989..2997
FT /note="Catalytic loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 3009..3033
FT /note="Activation loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT COILED 3..50
FT /evidence="ECO:0000255"
FT COILED 989..1020
FT /evidence="ECO:0000255"
FT COILED 2073..2102
FT /evidence="ECO:0000255"
FT COMPBIAS 1..20
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..97
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 313..329
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3157 AA; 360794 MW; D2B32B1B280AAF82 CRC64;
MPSEQFQEQQ RQQQQQYQQQ LERERQEKEQ NEKESLISNL VSLIIEIQQL GGNEYNSQNH
PINLSKDSIV DDKNNKKDLD TTSVTNNNKQ QQQQNKTTHK IQQKYELYIS RIRSILPELI
NSHLVSTASI SDINQIVKLI SYLIEKIPNS SLAIGDSSGD ICFFLFKLIP FLSHKSSTNI
CSQQHQQLDE NEEFTENLIT AIKAMLNFLN DDANVLLLFF KETICLFKDC IDFIKCLNSS
NNQQEQEKSI FPMVLKTYCE LSSSVIINSN TTGNTSSYSK HYQSLDLLVQ TEMAFNQYSK
NNNNNNNNNN NSEKDKNIEK EKSNSNDDNS KSIDEGVIIV ISSINRLINL QYGLLVSISS
VIDNFSPILY SVSIDLWDVL SQYLFLLNDL KLSSFKYMAN FIYSLLVLFE KLLKLSSNQI
PALLYNNIVG QFFNVLLPMS MLLNDKDCSK RREEIDIKLT SIIPLLFSNV PSSTLFDFSS
IILPNILFII NNTNLTELKV SILDIYLNIL KLSELQFKSI YNLVPLFGDE GIHDKLYQCF
DYVINQSYLI DTDDDDDDTD IDISLEILNT LIDYSNCLNI SNIYESISTV TNSVKDKNID
NNTENIEKDD GDGGAGGTSE NVNNKQIENQ KSITEDSKQQ QQQQQQQPTI EQQKSIRIYL
KALHLIFKIC KNHHNRVSVK ELVNLFINNL NFIYDLIIQQ KELDNLFLEV LMDLLKLSST
VEIEYLHRIS TLKSNEQLLE SLFKLSTIVT NHSTIFNLTK YAEIINSIKV ISFSLVCWLP
CSYYVNERVD IVLSTLSLNK SLVNSTTTTT TTTTTTTTTT TTTATTNTTT TTATTTDKSS
SQQNVGESNK NNPTIVSSSP ILVNISSDTS SSMGDSQAIS ASQQQQRNNN NNNKGEKYLS
KLDQNYLINV IKLLPVFISN MSPVNSLATI STNHQYQIMC NDIIKRLESI IDLNVTSLIL
DMVGVIGGSL YCLKEGYCFF ENLNRCTYLN NNNNNNNNNN NNNNNNNNNN NNIQEQNNLI
IINNSILLQR FTDTKNNRIK IDPLLSNYNN LVGCGIFEGV WLPLCSCKCV TVSSVLNTNI
VDLNLKPYRP IHKSPPSNNN NNNNEKDETI MDIDKTNNNE QILILESKYK ENILFSKLFL
EYHDGQNSDL DNDTSMMTSG GGDGDHDSSI KYFYLGFTFK NILIHNSPLP STSIEGFFKF
YLNLLSANRN DIIRKSVIKI IISMLKFNYQ TNPDVLTNFF YQLHPLYSNK SEREDILFSI
LYLLKKIANL IIQSQKDQYI LNNSLNNNNN NNNNNNVQQL SSSSSSHNNQ LNNNNTTTTN
NDSITWQDKI YSNDFILIIF CTLIENLSRQ TTIKVNSIET IEFIAKKLNQ TPKTLMMTNM
AQYINPRIIE NLYTNRQSLV ELCRYFLGIG LNQFLISSLP TVIPVLIFKS SNNILNCLKD
ELKDLKELCG QDYLMGLIFE YIFIHATSVE NLTSCIICFC RFADFNQDAV VSNLPKSFIN
SLILNLANGD GQQAKKALDY VSQFVGGDMT FDKFLIKEFL GSMNFFTMIM TNKQKSTQEK
LTMMTSFHRL LGLMGQSVDL FRPKIMAFLR LAIKTPMEEI AIDAWFTFIQ LLKLDSIGPI
LNQIVFSILP FVQSYPEKSL LIFDYLIIKN RTILKPYFKT IPFLPKEYTV LKPYIQELES
SSANLTTQIK QLMELFSNES IEVKLMSTNK LKELLSANKS IIHEMIQLDS DPILINDLVK
SLLIGCRDPY LKLSVNFLTC LGELGAIDPV KLDFSLRNQS TAEKDKVQIA AELIEKYLAK
FVLSPSNPTP QDRAGYAIQE ILKCCQDTKL SEKLSNECND IIKPYYSSEY MLPLFSKKNN
NLVGGGGGNG NGGGNGVSSS GSSSCFYGSN MTYKKWITSW LSDLLFKTKG SPGSANNDLD
SIFMACRGII KDDLKICHYL LPLVILNIIE YGRESDIQSI KNEILEVLNN NTDLSTDNGQ
ICTQIIFHVI NSLNNWIDVR KRKYFLTQQQ RTASQKGKSK KLESPPDVVV IERFLNGIPE
KILAFASYRC GALSTSLSYM ESLIRNETSQ ILKQQQQQHQ LNLQLNMQQQ NLQYQQQLQQ
QQPQQQQQQQ QQQQQQQVQQ QQIPILNSSQ PASMASAREL ALNNNLQLLQ KIYHEMDDID
GLIGLSHKRV GSPSNEEKIV ELESQGFWGD ALLYYNTALE RSPINMDLRI GALNCLFNLG
QHESLLLQIE GLKQEPWLTN KDQSKLGTIK IQSSWRLSHW DKIDETLSQT HEPNFEAYLG
RILYSLVKKQ EKEFNSNLFQ CRSFLAQLLN GSALDSYQRC YPYLIQAHIL EEIEQSYKLI
HYNVIPIENV TITNNNNNIG NNNNSNNNNV QKQQMLKKLL SEWDDRLKII QPSFKLRESI
LSVRREILEI GSFSKEVIDC WMKIGKYARH DKKFELSLNA ILKPTPSQQD KFYFIEHAKL
MWNQGHSFEA INILTQELKN KWNPIINSNN NSSNNSSSNI NINNGSKLSL LGLPQEDLFI
ISKTHLLIAR WKQQCGITHH SELTEHYSSA TAFEWEKGFF FLGRYYDSLL SNLKRVNQNS
PNSPPLDSVS YVDYTRNIIS SYGQAVILGH SYIYQTLTRI IALWCELGVI FSDFKIPEKS
TKVTSDSLES IKKAMSKLEL DVPASNWLMF LSQVASRICH KNTDTWLILE KIIIRTMIEF
PKQSIWIMMM QYRSSSSIRK QRARTCLEKA RKTDAIKKNQ DETILLCAQL LAVSNHPKKA
NKRYSIKMSE IFKKLYEMKD LTVILPLQSS LNVSLPPNGK PDKSYPAFGS LPLIGSFEDQ
IDIMPSLQGP KKINVNDRQG NRYSFLLKPN DDLRKDQRVM EFNTMVNKLI KKDPACRKRN
LKIRTYTVIP FNEESGIIEW VSNTATLRSI LGGIYETMDT IGIDRFNQMV NAKKEKHYLD
LFEDFNRFFP PVLHKHFITN FPEPSAWLDA RDAFARSCAI MSMVGSVLGL GDRHTENILL
DSITGECVHI DYNCLFWKGE TFTVPERVPF RLTRNMVDVF GVLGVEGTFK TVCENTLRVL
RSNKEVLLRV LENFIYDPFL ILFDKKNQNS NNNNNTHFGE VENDQAVEIL KRISATLQGI
PTVGLQLSIE GQVNHLIQEA TNPKHLCNMY VGWCSWY
//
