GenomeNet

Database: UniProt
Entry: Q54F05
LinkDB: Q54F05
Original site: Q54F05 
ID   DHX8_DICDI              Reviewed;        1160 AA.
AC   Q54F05;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   16-OCT-2019, entry version 104.
DE   RecName: Full=ATP-dependent RNA helicase dhx8;
DE            EC=3.6.4.13;
DE   AltName: Full=DEAH box protein 8;
GN   Name=dhx8; Synonyms=prp22; ORFNames=DDB_G0291183;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelids;
OC   Dictyosteliales; Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Facilitates nuclear export of spliced mRNA by releasing
CC       the RNA from the spliceosome. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Identified in the spliceosome complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH
CC       subfamily. DDX8/PRP22 sub-subfamily. {ECO:0000305}.
DR   EMBL; AAFI02000175; EAL61875.1; -; Genomic_DNA.
DR   RefSeq; XP_635385.1; XM_630293.1.
DR   SMR; Q54F05; -.
DR   STRING; 44689.DDB0233399; -.
DR   PaxDb; Q54F05; -.
DR   PRIDE; Q54F05; -.
DR   EnsemblProtists; EAL61875; EAL61875; DDB_G0291183.
DR   GeneID; 8628033; -.
DR   KEGG; ddi:DDB_G0291183; -.
DR   dictyBase; DDB_G0291183; dhx8.
DR   eggNOG; KOG0922; Eukaryota.
DR   eggNOG; COG1185; LUCA.
DR   eggNOG; COG1643; LUCA.
DR   InParanoid; Q54F05; -.
DR   KO; K12818; -.
DR   OMA; RIQKAIC; -.
DR   PhylomeDB; Q54F05; -.
DR   PRO; PR:Q54F05; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   Proteomes; UP000002195; Unassembled WGS sequence.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005681; C:spliceosomal complex; ISS:dictyBase.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000390; P:spliceosomal complex disassembly; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR   InterPro; IPR011709; DUF1605.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF07717; OB_NTP_bind; 1.
DR   Pfam; PF00575; S1; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00847; HA2; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing;
KW   mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN         1   1160       ATP-dependent RNA helicase dhx8.
FT                                /FTId=PRO_0000330832.
FT   DOMAIN      202    274       S1 motif. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00180}.
FT   DOMAIN      518    681       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      699    879       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     531    538       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       628    631       DEAH box.
FT   COMPBIAS     75     79       Poly-Thr.
FT   COMPBIAS     80     91       Poly-Asn.
FT   COMPBIAS     95    106       Poly-Thr.
FT   COMPBIAS    107    110       Poly-Asn.
FT   COMPBIAS    309    314       Poly-Asn.
FT   COMPBIAS    317    321       Poly-Asn.
FT   COMPBIAS    322    325       Poly-Asp.
SQ   SEQUENCE   1160 AA;  131707 MW;  FE7E72B8D93617FA CRC64;
     MDKLERIELE SQVCNELERF IGSGDKLLAE FVIGLADENP KLKDFNKAIS ENVPDFPESL
     SSHLFNLIEK MKKKTTTTTN NNNNNNNNNT NTAKTTTTTT TTTTTTNNNN YKESEWEETK
     LNSNSNNQKK NQFPGLSIPN KVEWDQGKIV DVPIDDEKTK EELKRKQQDM DREFEREQRE
     KRDRDREQQN KRREIDKEPI LYKIYDGKVS SINDYGCFVT LEGIAGRRDG LVHISQILSG
     RTKLNHPSDV VKRNQQVKVK ILSVASSKIS LSMKDVDQST GRDLNPQQNI QSIISTNSTN
     NRSNPFKPNN NNNNSSNNNN NDDDDKYTTS KNRKRIASPD RWGYKQLIAS GILSVPEMPN
     YDKEVGLVNH DEEQPEEDFD IERNEDEPQF LKGTRMNMQQ LSPIKIVKKP NGSLQRAAST
     QTALSKERKE EKNQQRNEMM DSIPKDLSLP WHDPMPEAGE RHLAQEIRSI AGQGIDTEIP
     EWKKVTQGSH IQYGKATSRS IKEQRESLPI FPLREAFLQA VSEHQLLVVI GETGSGKTTQ
     MAQYLAEAGY GTRGKIGCTQ PRRVAAMSVS KRVAEEFGCQ LGQEVGYAIR FEDCTSPETI
     IKFMTDGILL RECLLDPNLS AYSVIILDEA HERTISTDVL FGLLKQALQR RPELKVLITS
     ATLEAEKFSK YFMNAQLFII PGRTFPVDIR YTKDPEADYL DASLITVMQI HLSEPPGDIL
     LFLTGQEEID AACQILYERM KSLGSNVPDL IILPVYSALP SEMQTKIFEP APPGSRKVVI
     ATNIAETSLT IDGIYYVIDP GFSKQKCFNP KNGMDSLVVA PISQAAARQR SGRAGRTGPG
     KCYRLYTESA FKNEMLASSI PEIQRTNLGN TVLTMKAMGI NDLLNFDFMD PPPVQTLVSA
     MEQLYSLGAL DEEGLLTRLG RKMAEFPLDP QLSKMLIASV DLGCSDEILT VVAMLSVQNV
     FYRPKEKQAL ADQKKAKFFQ PEGDHLTLLN VYESWKNSKF SNPWCFENFV QARSLRRAQD
     VRKQLITIMD RYKLDIISAG RNYTKIQKAI CSGFFANASK KDPNEGYKTL VEGQPVYIHP
     SSTLFNRNPD WVIYHELVMT TKEYMREVCT IDPKWLVELA PKFFKTSDPN KISKRKRKEK
     IEPLYDKYND PNAWRPSKRK
//
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