GenomeNet

Database: UniProt
Entry: Q54MH3
LinkDB: Q54MH3
Original site: Q54MH3 
ID   DHX16_DICDI             Reviewed;        1106 AA.
AC   Q54MH3;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   16-OCT-2019, entry version 109.
DE   RecName: Full=Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16;
DE            EC=3.6.4.13;
DE   AltName: Full=DEAH-box protein 16;
GN   Name=dhx16; ORFNames=DDB_G0285937;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelids;
OC   Dictyosteliales; Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC       spliceosome. Contributes to pre-mRNA splicing after spliceosome
CC       formation and prior to the first transesterification reaction.
CC       {ECO:0000250|UniProtKB:O60231}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Component of pre-catalytic spliceosome complexes.
CC       {ECO:0000250|UniProtKB:O60231}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O60231}.
CC       Nucleus, nucleoplasm {ECO:0000250|UniProtKB:O60231}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH
CC       subfamily. DDX16/PRP8 sub-subfamily. {ECO:0000305}.
DR   EMBL; AAFI02000082; EAL64503.1; -; Genomic_DNA.
DR   RefSeq; XP_638017.1; XM_632925.1.
DR   SMR; Q54MH3; -.
DR   STRING; 44689.DDB0233398; -.
DR   PaxDb; Q54MH3; -.
DR   EnsemblProtists; EAL64503; EAL64503; DDB_G0285937.
DR   GeneID; 8625368; -.
DR   KEGG; ddi:DDB_G0285937; -.
DR   dictyBase; DDB_G0285937; dhx16.
DR   eggNOG; KOG0923; Eukaryota.
DR   eggNOG; COG1643; LUCA.
DR   InParanoid; Q54MH3; -.
DR   KO; K12813; -.
DR   OMA; VYNQWVE; -.
DR   PhylomeDB; Q54MH3; -.
DR   Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR   PRO; PR:Q54MH3; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   Proteomes; UP000002195; Unassembled WGS sequence.
DR   GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; ISS:dictyBase.
DR   GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR   InterPro; IPR011709; DUF1605.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF07717; OB_NTP_bind; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00847; HA2; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing;
KW   mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN         1   1106       Putative pre-mRNA-splicing factor ATP-
FT                                dependent RNA helicase DHX16.
FT                                /FTId=PRO_0000330890.
FT   DOMAIN      477    640       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      665    838       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     490    497       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       587    590       DEAH box.
FT   COMPBIAS     79     95       Poly-Ser.
FT   COMPBIAS     96    164       Lys-rich.
FT   COMPBIAS    187    201       Poly-Asn.
FT   COMPBIAS    216    224       Poly-Asn.
FT   COMPBIAS    327    330       Poly-Lys.
FT   COMPBIAS    378    383       Poly-Ser.
SQ   SEQUENCE   1106 AA;  126564 MW;  428EE89A2AAFA115 CRC64;
     MSSNNESIKN WVSDKIFDIL GYRESTMVDY IIALSKKAKD VNSFISTLTE QDFPINSNTK
     SFAQELLNKS QQKIQNITSS SSSSSSTSLS SSSSSKDKEK EKIEFLKKNK SYKLVIDHDD
     DIVNSSGSSD SDSDSERKRK KKEKKKEKKD KKDKKDKKSS TRKKSDNNWD DEIEPEPIKP
     NEKEDENNNN ENNDNNNDNN NLQKRQPYKS IIEEENNNDN NNNNGEEDEY EREQREVKEL
     SDRIKKRDEK STKKKIVDDS ETKESIERKN RLEQNEQLET ERTKSRRKYL VGEEQKRLIL
     LKREIEEEYE LFKDQKLTEQ EIKDFEKKKK LYELASQRIN ESQQSDDYYQ LPSEIKDKDS
     LLKSSYINDN KNKKGNDSSS SSSYNPEQKE WEQNRMKSAI SENRGLSTAN IGGGNEEYEY
     VFEDQIEFIK EEVLKQGQKG DGVMILKPGD DGSAQAKMTI QEVRKSLPVY PYREQLIDAV
     REYQVLIIVG ETGSGKTTQI PQYLHEAGFS KTGKIGCTQP RRVAAMSVAA RVAEEVGCKL
     GNEVGYSIRF EDCTSQKTVL QYMTDGMLVR EFLTAPDLAS YSVLIIDEAH ERTLHTDILF
     GLLKDITRFR PDLKLLISSA TMDAERFSDY FDGAPTFNIP GRKYEVTTHY TQAPEADYLD
     AAVVTVLQIH ITEPLGDILV FLTGQEEVDQ AAEMLQTRTR GLGTKIKELI ITRIYSTLPT
     DLQAKIFEPT PPNARKVVLA TNIAETSLTI DGIIYVIDPG FCKQKMFNPR TGMESLVITP
     VSRASANQRK GRAGRVAPGK CFRLFTAWAF DNELEENTIP EIQRTNLGNV VLLLKSMGIN
     DLMNFDFMDP PPAQTLIAAL EQLYALGALN DRGQLTKLGR KMAEFPVDPQ LSKMIIASEK
     YKCSEEILTI CAMLSVGNTI FYRPKDKAFA ADAARKLFFH PQGDHLTLMN VFNQWRESGY
     AVQWCFENFI QHRSMKRAQD VRDQLELLLE RVEIPLVSNV DDTDSIRKCI ASGFFYNSAK
     LEKSGLFRTT KHNQSVQIHP SSCLFQSPPK WVVYHELVLT TKEFMRQIVE IQSSWLHEIA
     PHIYKEKDVN DNQKLPKNIG KKQINK
//
DBGET integrated database retrieval system