ID Q57VF3_TRYB2 Unreviewed; 1249 AA.
AC Q57VF3; D6XG03;
DT 10-MAY-2005, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2005, sequence version 1.
DT 27-MAR-2024, entry version 106.
DE RecName: Full=adenylate cyclase {ECO:0000256|ARBA:ARBA00012201};
DE EC=4.6.1.1 {ECO:0000256|ARBA:ARBA00012201};
DE AltName: Full=ATP pyrophosphate-lyase {ECO:0000256|ARBA:ARBA00032597};
DE AltName: Full=Adenylyl cyclase {ECO:0000256|ARBA:ARBA00032637};
GN Name=Tb05.30F7.530 {ECO:0000313|EMBL:AAZ11180.1};
GN ORFNames=Tb927.5.650 {ECO:0000313|EMBL:AAX70416.1};
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431 {ECO:0000313|EMBL:AAX70416.1, ECO:0000313|Proteomes:UP000008524};
RN [1] {ECO:0000313|EMBL:AAZ11180.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:AAZ11180.1};
RX PubMed=16020724; DOI=10.1126/science.1112181;
RA El-Sayed N.M., Myler P.J., Blandin G., Berriman M., Crabtree J.,
RA Aggarwal G., Caler E., Renauld H., Worthey E.A., Hertz-Fowler C.,
RA Ghedin E., Peacock C., Bartholomeu D.C., Haas B.J., Tran A.N.,
RA Wortman J.R., Alsmark U.C., Angiuoli S., Anupama A., Badger J.,
RA Bringaud F., Cadag E., Carlton J.M., Cerqueira G.C., Creasy T.,
RA Delcher A.L., Djikeng A., Embley T.M., Hauser C., Ivens A.C.,
RA Kummerfeld S.K., Pereira-Leal J.B., Nilsson D., Peterson J., Salzberg S.L.,
RA Shallom J., Silva J.C., Sundaram J., Westenberger S., White O.,
RA Melville S.E., Donelson J.E., Andersson B., Stuart K.D., Hall N.;
RT "Comparative genomics of trypanosomatid parasitic protozoa.";
RL Science 309:404-409(2005).
RN [2] {ECO:0000313|EMBL:AAZ11180.1, ECO:0000313|Proteomes:UP000008524}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:AAZ11180.1,
RC ECO:0000313|Proteomes:UP000008524};
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [3] {ECO:0000313|EMBL:AAX70416.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GUTat10.1 {ECO:0000313|EMBL:AAX70416.1};
RA Ghedin E., Blandin G., Bartholomeu D., Caler E., Haas B., Hannick L.,
RA Shallom J., Hou L., Djikeng A., Feldblyum T., Hostetler J., Johnson J.,
RA Jones K., Koo H.L., Larkin C., Pai G., Peterson J., Khalak H.G.,
RA Salzberg S., Simpson A.J., Tallon L., Van Aken S., Wanless D., White O.,
RA Wortman J., Fraser C.M., El-Sayed N.M.A.;
RT ".";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:AAZ11180.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:AAZ11180.1};
RA Ghedin E., Blandin G., Bartholomeu D., Caler E., Haas B., Hannick L.,
RA Shallom J., Hou L., Djikeng A., Feldblyum T., Hostetler J., Johnson J.,
RA Jones K., Koo H.L., Larkin C., Pai G., Peterson J., Khalak H.G.,
RA Salzberg S., Simpson A.J., Tallon L., Van Aken S., Wanless D., White O.,
RA Wortman J., Fraser C.M., El-Sayed N.M.A.;
RT "Sequencing, closure, and annotation of Trypanosoma brucei chromosomes 2
RT through 8.";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Could act as a receptor for an unknown ligand.
CC {ECO:0000256|ARBA:ARBA00002708}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP = 3',5'-cyclic AMP + diphosphate; Xref=Rhea:RHEA:15389,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58165; EC=4.6.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001593};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the adenylyl cyclase class-3 family.
CC {ECO:0000256|ARBA:ARBA00005381}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC159441; AAX70416.1; -; Genomic_DNA.
DR EMBL; CP000068; AAZ11180.1; -; Genomic_DNA.
DR RefSeq; XP_844739.1; XM_839646.1.
DR AlphaFoldDB; Q57VF3; -.
DR STRING; 185431.Q57VF3; -.
DR PaxDb; 5691-AAZ11180; -.
DR GeneID; 3657174; -.
DR KEGG; tbr:Tb927.5.650; -.
DR eggNOG; KOG0618; Eukaryota.
DR InParanoid; Q57VF3; -.
DR OrthoDB; 66776at2759; -.
DR Proteomes; UP000008524; Chromosome 5.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004016; F:adenylate cyclase activity; ISM:GeneDB.
DR GO; GO:0006171; P:cAMP biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0035556; P:intracellular signal transduction; ISM:GeneDB.
DR Gene3D; 3.40.50.2300; -; 2.
DR Gene3D; 3.30.70.1230; Nucleotide cyclase; 1.
DR InterPro; IPR001054; A/G_cyclase.
DR InterPro; IPR029787; Nucleotide_cyclase.
DR InterPro; IPR028082; Peripla_BP_I.
DR PANTHER; PTHR43081; ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED; 1.
DR PANTHER; PTHR43081:SF1; PH-SENSITIVE ADENYLATE CYCLASE RV1264; 1.
DR Pfam; PF00211; Guanylate_cyc; 1.
DR SMART; SM00044; CYCc; 1.
DR SUPFAM; SSF55073; Nucleotide cyclase; 1.
DR SUPFAM; SSF53822; Periplasmic binding protein-like I; 2.
DR PROSITE; PS50125; GUANYLATE_CYCLASE_2; 1.
PE 3: Inferred from homology;
KW cAMP biosynthesis {ECO:0000256|ARBA:ARBA00022998};
KW Lyase {ECO:0000313|EMBL:AAX70416.1}; Membrane {ECO:0000256|SAM:Phobius};
KW Receptor {ECO:0000256|ARBA:ARBA00023170, ECO:0000313|EMBL:AAX70416.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000008524};
KW Signal {ECO:0000256|SAM:SignalP}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..1249
FT /note="adenylate cyclase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010139567"
FT TRANSMEM 815..838
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 859..1013
FT /note="Guanylate cyclase"
FT /evidence="ECO:0000259|PROSITE:PS50125"
SQ SEQUENCE 1249 AA; 136086 MW; 439E9349838501A9 CRC64;
MRLIVLFLLS LLMVAPVVCV ADNVTVKVYS LLYDPFLPDV YNNGVNAGLH ASFAARQWST
ASNVNVEVIH PLSYEVPPPE LLQSIIEENK NEFFVVVGPL SDSDTLSVLP SLEEEDLVAF
APFTGSDAVR GWSANAYFLH VSPAAELLAL LRYAVSQLHL LRIGFMYLQN VHFGDSEYEL
AVELMSQMGR SLCGVFTLES SFDGEADDAE FTATWELFAG THPQGVMIFA PQVSDAMRFL
MKLVTDNRTN SAYILSPTPH VTNIESAWTL AGATSGVKMF PGQVVLSGVL PLVSDDGFRA
TRRLRADIKA YALSGTGAVE FDPLAFHGDA AYGGQVMFGW IVGEVLAQAL QCSEFLKSRM
TFMDSLYNQR RYVIDDIVIG DFGGECEPLA AAYGATCYCN EGGRMIYMMI LGDDYLPRPA
ADGLITFDAC DESGVQMLAP LYTLLFSSVN DPFARSVNGA IHRGALFVSG NGHLGKSERL
FIHSVPSTSG NVMSDLRKML DTRAVTSVLG VVDDATLSTP DVVFIDPITL NPRLRHPGRN
VIYLSPTLEQ QLFVIAGYLA SENASTLHAV MRDDATRLIE TVVNRTLLSF NRSLDSVVSL
DRDAPLNAAL PTDGSLLLIG LTASDVGGIA AHLSANRNVR VFIPFFDVAL FYDDFIRAFR
GLKSAERLIF ATNLPHWADH RPSSETIRRF HANRPNASDR TPLALLGFTS ASFLDAVAQH
IVGVDPQKVL TTIYTHSVIS VDDMQYGAFA DEDCSRIVGN HTDAVDGCLV NYGATHISLW
PLARALNAAV PPLTKPETPS MHYHNLEEDD HPKSVLAGLV AGVLSVVVML AVLLLVLFQL
RGGARDNVNA PKELTGPVTL IFTDIESSTA QWAAHPEMMP DAVATHHRLI RALIMHYRCY
EVKTVGDSFM IACHNPFNAC QLACCLQRCF LQHTWGTAAF DESYRQIECQ RALENEEYAP
PTGYLELSVY QKLWCGLRVR VGIHTGLCDI RCDEVTKGYD YYGRTSIIAA QTESIANGGQ
VLLTHATYMS LKTAEREQLN VTSLGPVPLC GVPEPVVMYQ LEAVPGRAFA ELRIGHDSGA
AGACGTASET GSLVVELSDE AQVVATSLES VLSTFAPAQR RKALMTFCER WRVSLPRNAK
EVWDDAFCCD VIRVIAAKAC RIAEYEPRST SNSVTQTPGH SFALPSRVFG SVSLGSQLPL
APPRVCDAVN EAPCPDATSS SNCDNSSESY VIVVEFPEGF GENTSRNGE
//
