GenomeNet

Database: UniProt
Entry: Q580W5
LinkDB: Q580W5
Original site: Q580W5 
ID   XPB_TRYB2               Reviewed;         938 AA.
AC   Q580W5; D6XE64;
DT   31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   16-OCT-2019, entry version 94.
DE   RecName: Full=TFIIH basal transcription factor complex helicase XPB subunit;
DE            EC=3.6.4.12;
GN   Name=XPB {ECO:0000303|PubMed:17259543}; ORFNames=Tb927.3.5100;
OS   Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=185431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=927/4 GUTat10.1;
RX   PubMed=16020726; DOI=10.1126/science.1112642;
RA   Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA   Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B.,
RA   Bohme U., Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L.,
RA   Wickstead B., Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J.,
RA   Bringaud F., Brooks K., Carrington M., Cherevach I.,
RA   Chillingworth T.J., Churcher C., Clark L.N., Corton C.H., Cronin A.,
RA   Davies R.M., Doggett J., Djikeng A., Feldblyum T., Field M.C.,
RA   Fraser A., Goodhead I., Hance Z., Harper D., Harris B.R., Hauser H.,
RA   Hostetler J., Ivens A., Jagels K., Johnson D., Johnson J., Jones K.,
RA   Kerhornou A.X., Koo H., Larke N., Landfear S., Larkin C., Leech V.,
RA   Line A., Lord A., Macleod A., Mooney P.J., Moule S., Martin D.M.,
RA   Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA   Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E.,
RA   Rajandream M.A., Reitter C., Salzberg S.L., Sanders M., Schobel S.,
RA   Sharp S., Simmonds M., Simpson A.J., Tallon L., Turner C.M., Tait A.,
RA   Tivey A.R., Van Aken S., Walker D., Wanless D., Wang S., White B.,
RA   White O., Whitehead S., Woodward J., Wortman J., Adams M.D.,
RA   Embley T.M., Gull K., Ullu E., Barry J.D., Fairlamb A.H.,
RA   Opperdoes F., Barrell B.G., Donelson J.E., Hall N., Fraser C.M.,
RA   Melville S.E., El-Sayed N.M.A.;
RT   "The genome of the African trypanosome Trypanosoma brucei.";
RL   Science 309:416-422(2005).
RN   [2]
RP   IDENTIFICATION IN TFIIH COMPLEX.
RX   PubMed=17259543; DOI=10.1128/ec.00411-06;
RA   Lee J.H., Nguyen T.N., Schimanski B., Guenzl A.;
RT   "Spliced leader RNA gene transcription in Trypanosoma brucei requires
RT   transcription factor TFIIH.";
RL   Eukaryot. Cell 6:641-649(2007).
CC   -!- FUNCTION: ATP-dependent 3'-5' DNA helicase, component of the
CC       general transcription factor IIH (TFIIH) core complex, involved in
CC       spliced leader RNA (SL RNA) gene transcription by RNA polymerase
CC       II (PubMed:17259543). TFIIH has an essential role in transcription
CC       initiation. When the pre-initiation complex (PIC) has been
CC       established, TFIIH is required for promoter opening and promoter
CC       escape. The ATP-dependent helicase activity of XPB is required for
CC       promoter opening and promoter escape (By similarity).
CC       {ECO:0000250|UniProtKB:Q00578, ECO:0000305|PubMed:17259543}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC       XPB, XPD, SSL1, TFB1, TFB2, TFB4 and TFB5.
CC       {ECO:0000250|UniProtKB:Q00578, ECO:0000305|PubMed:17259543}.
CC   -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
CC       {ECO:0000305}.
DR   EMBL; AC093543; AAX81019.1; -; Genomic_DNA.
DR   EMBL; CP000066; AAZ10541.1; -; Genomic_DNA.
DR   RefSeq; XP_844100.1; XM_839007.1.
DR   STRING; 5691.AAZ10541; -.
DR   PaxDb; Q580W5; -.
DR   GeneDB; Tb927.3.5100:pep; -.
DR   GeneID; 3656453; -.
DR   KEGG; tbr:Tb927.3.5100; -.
DR   EuPathDB; TriTrypDB:Tb927.3.5100; -.
DR   HOGENOM; HOG000134498; -.
DR   InParanoid; Q580W5; -.
DR   KO; K10843; -.
DR   OMA; IRADCYY; -.
DR   Proteomes; UP000008524; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR   GO; GO:0005654; C:nucleoplasm; IDA:GeneDB.
DR   GO; GO:0000112; C:nucleotide-excision repair factor 3 complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:GeneDB.
DR   GO; GO:0005675; C:transcription factor TFIIH holo complex; IBA:GO_Central.
DR   GO; GO:0097550; C:transcriptional preinitiation complex; IBA:GO_Central.
DR   GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:GeneDB.
DR   GO; GO:0008134; F:transcription factor binding; IBA:GO_Central.
DR   GO; GO:0033683; P:nucleotide-excision repair, DNA incision; IBA:GO_Central.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:GOC.
DR   GO; GO:0009411; P:response to UV; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR   InterPro; IPR032438; ERCC3_RAD25_C.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001161; XPB/Ssl2.
DR   Pfam; PF16203; ERCC3_RAD25_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00603; rad25; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN         1    938       TFIIH basal transcription factor complex
FT                                helicase XPB subunit.
FT                                /FTId=PRO_0000442798.
FT   DOMAIN      394    562       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      627    781       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     407    414       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       515    518       DEVH box. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
SQ   SEQUENCE   938 AA;  105211 MW;  FB66A3C193DFCE37 CRC64;
     MILGPGGRIF INHGHPAYPH LMDFLTACCE PVCRTLYVSE YTISPSSLSA ATAEGTYSME
     MVRNVIRYFR LDEQQQIPVD VERYAALERR VRDSVQDTSL DLPMEVGEAK VSANGDVKAE
     EGCEEATDEL SPLAGQVKKE ETKEVAAEPR RRFLSLSKRI SAKSEPLVTR AVVNTGALQP
     LPADLEQMLR EEENSSRVRI VLQPCLRPVK RRAVGGDKQH SDEQQCQRAE ETKLAYFLTS
     PDRNHMEHLV SRLQDFLVPV LLHGTRRWVV SDVDRGVEER STAESGRAKT LRRLFEAPSS
     ASGRSVASKS LTNEGANGDG LGGGVGRRCT RIVYKSQVMD GKMRNVRERL YKELSVRADL
     FYDYVQDHSL HVCDLELSEN VRLRPYQVAS LERFRSGNKA HQGVIVLPCG AGKTLTGIGA
     AATVKKRTIV MCINVMSVLQ WQREFIRWTN LSEDQVTVCI ADKKQMPGDV FITTYSMLIA
     RRSNVPEMEQ SADAKLTAKI LASVGEQPWG LLLLDEVHTA LAHNFQEVLN KVKYKCVIGL
     SATLLREDDK IGDLRHLVGP KLYEANWLDL TRAGFLARVE CAEIQCPLPK AFLTEYLESQ
     SDGDPFARRG TTRMAHSVVC LNPYKLWCTQ ALLEFHRNRS PPDKVIIFCD QIDGIQYYAQ
     HLHVPFMDGK TSDMERENLL QYFQHSDNIN AIILSRVGDV ALDIPCASVV IQISGLGASR
     RQEAQRLGRI LRPKPASLDN VCSYFYTLVS QDTHEISQSY ERQSWLRDQG FSYRVLQSDM
     VLQHFLRTGG KLCCVGPPRW WYECAGPSCD SAVAAKGTYW IPFSQEAALR MQSRFVAGVR
     GCDLTATVLR DTPRPPELKN MGVEEKWTVC FSDSCAPETF GTVQLVEGNP LLVRRICCGP
     LAVEHDCLHG GEECLQYAVQ QMKVMVAKNS KNRIPLTT
//
DBGET integrated database retrieval system