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Database: UniProt
Entry: Q59W52
LinkDB: Q59W52
Original site: Q59W52 
ID   PRP28_CANAL             Reviewed;         581 AA.
AC   Q59W52; A0A1D8PF71;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 2.
DT   31-JUL-2019, entry version 85.
DE   RecName: Full=Pre-mRNA-splicing ATP-dependent RNA helicase PRP28;
DE            EC=3.6.4.13;
GN   Name=PRP28; OrderedLocusNames=CAALFM_C111300CA;
GN   ORFNames=CaO19.672, CaO19.8289;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Debaryomycetaceae;
OC   Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S.,
RA   Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T.,
RA   Davis R.W., Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs
RT   aligned on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME
RP   REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates
RT   allele-specific measurements and provides a simple model for repeat
RT   and indel structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved in mRNA splicing.
CC       May destabilize the U1/5'-splice site duplex to permit an
CC       effective competition for the 5'-splice site by the U6 snRNA,
CC       resulting in the switch between U1 and U6 at the 5'-splice site.
CC       May also act to unwind the U4/U6 base-pairing interaction in the
CC       U4/U6/U5 snRNP, facilitating the first covalent step of splicing
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Component of the U5 snRNP complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus
CC       {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX23/PRP28
CC       subfamily. {ECO:0000305}.
DR   EMBL; CP017623; AOW26755.1; -; Genomic_DNA.
DR   RefSeq; XP_713805.2; XM_708712.2.
DR   SMR; Q59W52; -.
DR   STRING; 5476.C4YCS2; -.
DR   PRIDE; Q59W52; -.
DR   GeneID; 3644552; -.
DR   KEGG; cal:CAALFM_C111300CA; -.
DR   CGD; CAL0000181927; orf19.672.
DR   EuPathDB; FungiDB:C1_11300C_A; -.
DR   InParanoid; Q59W52; -.
DR   KO; K12858; -.
DR   OrthoDB; 820037at2759; -.
DR   PRO; PR:Q59W52; -.
DR   Proteomes; UP000000559; Chromosome 1.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase;
KW   mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN         1    581       Pre-mRNA-splicing ATP-dependent RNA
FT                                helicase PRP28.
FT                                /FTId=PRO_0000232371.
FT   DOMAIN      199    396       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      424    575       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     212    219       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       168    196       Q motif.
FT   MOTIF       328    331       DEAD box.
FT   COMPBIAS     59     79       Thr-rich.
SQ   SEQUENCE   581 AA;  65641 MW;  DAE95408BF543F81 CRC64;
     MSSNIFKRFK SAEESLDEDE SVTPVYIPKA KRAKLEALKA KKSQIASRLS THKQQSAPTT
     TTNTSTTAST TTTATTNNTN NSKPSIQTKK PTGKKFQFDW DVEEEDTASG FVPLIEMDEE
     IDDPLFSGQE STHWTNKNLE EMTDRDWRIF KEDYNITTKG KNIPNPLRYW NEGSINDKLV
     SIISQLGYEE PTSVQRASIP LALKKRDVVG VAETGSGKTL AFLIPVLNYI LSIDENYLKY
     EKISNEPVGL ILAPTRELAL QITKEAEKFC KKLGYQVLPI IGGHHYQDTI NKIDQTGVHL
     IVATPGRLVD SIERKIVDLS KCYCLVMDEA DRMIDMGFEK DLNKVLDKLP TEKQLSSTID
     GRIFHLEKRS TMMFTATISP TVEKLTKKYL IDPGYLYIGG AGEALDNISQ SFEFLSSATT
     EATKFNKLIK IIRSHWRVTE NPLIIIFANF KHVCDSLSQE LSSNDINNVV IHGSKSQDMR
     EQAITNFRNH ESEVLIATDV AARGIDIPNV TLVINYQMVK KFDEYIHRIG RTGRAGNLGE
     SFTFISDQDT EIFTPLKKFL KKGGKRLPDW LYKHQSQTVQ E
//
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