ID DID_ECHCA Reviewed; 64 AA.
AC Q5EE07;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 22-FEB-2023, entry version 52.
DE RecName: Full=Disintegrin;
DE Flags: Fragment;
OS Echis carinatus (Saw-scaled viper).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Viperidae; Viperinae; Echis.
OX NCBI_TaxID=40353;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RA Baskar Singh S., Srinivasan A., Singh T.P.;
RT "Disintegrin from the venom glands of Echis carinatus.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS), AND DISULFIDE BONDS.
RC TISSUE=Venom;
RA Hassan M.I., Ethayathulla A.S., Bilgrami S., Singh B., Yadav S.,
RA Singh T.P.;
RT "Crystal structure of a novel disintegrin from saw-scaled viper at 3.2 A
RT resolution.";
RL Submitted (MAR-2005) to the PDB data bank.
CC -!- FUNCTION: Inhibits adhesion of cells expressing alpha-4/beta-1
CC (ITGA4/ITGB1) and alpha-4/beta-7 (ITGA4/ITGB7) integrins to the natural
CC ligands vascular cell adhesion molecule 1 (VCAM-1) and mucosal
CC addressin cell adhesion molecule 1 (MADCAM-1). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer; disulfide-linked. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- SIMILARITY: Belongs to the disintegrin family. Dimeric disintegrin
CC subfamily. {ECO:0000305}.
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DR EMBL; AY902454; AAW82478.1; -; mRNA.
DR PDB; 1Z1X; X-ray; 3.20 A; A=1-64.
DR PDBsum; 1Z1X; -.
DR AlphaFoldDB; Q5EE07; -.
DR SMR; Q5EE07; -.
DR EvolutionaryTrace; Q5EE07; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 4.10.70.10; Disintegrin domain; 1.
DR InterPro; IPR018358; Disintegrin_CS.
DR InterPro; IPR001762; Disintegrin_dom.
DR InterPro; IPR036436; Disintegrin_dom_sf.
DR Pfam; PF00200; Disintegrin; 1.
DR PRINTS; PR00289; DISINTEGRIN.
DR SMART; SM00050; DISIN; 1.
DR SUPFAM; SSF57552; Blood coagulation inhibitor (disintegrin); 1.
DR PROSITE; PS00427; DISINTEGRIN_1; 1.
DR PROSITE; PS50214; DISINTEGRIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell adhesion impairing toxin; Disulfide bond; Secreted;
KW Toxin.
FT CHAIN <1..>64
FT /note="Disintegrin"
FT /id="PRO_0000322602"
FT DOMAIN <1..>64
FT /note="Disintegrin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT MOTIF 42..44
FT /note="Cell attachment site; atypical (MLD)"
FT DISULFID 6..29
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068,
FT ECO:0000269|Ref.2"
FT DISULFID 7
FT /note="Interchain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT DISULFID 12
FT /note="Interchain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT DISULFID 20..26
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068,
FT ECO:0000269|Ref.2"
FT DISULFID 25..50
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068,
FT ECO:0000269|Ref.2"
FT DISULFID 38..57
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068,
FT ECO:0000269|Ref.2"
FT NON_TER 1
FT NON_TER 64
FT STRAND 5..8
FT /evidence="ECO:0007829|PDB:1Z1X"
FT TURN 9..12
FT /evidence="ECO:0007829|PDB:1Z1X"
FT STRAND 21..23
FT /evidence="ECO:0007829|PDB:1Z1X"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:1Z1X"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:1Z1X"
SQ SEQUENCE 64 AA; 7127 MW; D65FC1B3FD393054 CRC64;
NSVHPCCDPV KCEPREGEHC ISGPCCRNCK FLNAGTICKR AMLDGLHDYC TGVTSDCPRN
RYNH
//
