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Database: UniProt
Entry: Q5HME0
LinkDB: Q5HME0
Original site: Q5HME0 
ID   CSHA_STAEQ              Reviewed;         509 AA.
AC   Q5HME0;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   16-OCT-2019, entry version 105.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase CshA {ECO:0000255|HAMAP-Rule:MF_01493};
DE            EC=3.6.4.13 {ECO:0000255|HAMAP-Rule:MF_01493};
GN   Name=cshA {ECO:0000255|HAMAP-Rule:MF_01493};
GN   OrderedLocusNames=SERP1688;
OS   Staphylococcus epidermidis (strain ATCC 35984 / RP62A).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176279;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35984 / RP62A;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T.,
RA   Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J.,
RA   Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S.,
RA   Haft D.H., Vamathevan J.J., Khouri H., Utterback T.R., Lee C.,
RA   Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H.,
RA   Hance I.R., Nelson K.E., Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete
RT   genome analysis of an early methicillin-resistant Staphylococcus
RT   aureus strain and a biofilm-producing methicillin-resistant
RT   Staphylococcus epidermidis strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- FUNCTION: DEAD-box RNA helicase possibly involved in RNA
CC       degradation. Unwinds dsRNA in both 5'- and 3'-directions, has RNA-
CC       dependent ATPase activity. {ECO:0000255|HAMAP-Rule:MF_01493}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01493};
CC   -!- SUBUNIT: Oligomerizes, may be a member of the RNA degradosome.
CC       {ECO:0000255|HAMAP-Rule:MF_01493}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01493}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. CshA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01493}.
DR   EMBL; CP000029; AAW55051.1; -; Genomic_DNA.
DR   RefSeq; WP_002457112.1; NC_002976.3.
DR   SMR; Q5HME0; -.
DR   STRING; 176279.SERP1688; -.
DR   EnsemblBacteria; AAW55051; AAW55051; SERP1688.
DR   KEGG; ser:SERP1688; -.
DR   eggNOG; ENOG4105C1J; Bacteria.
DR   eggNOG; COG0513; LUCA.
DR   HOGENOM; HOG000268810; -.
DR   KO; K05592; -.
DR   OMA; RNPIRIL; -.
DR   OrthoDB; 626183at2; -.
DR   BioCyc; SEPI176279:G1G46-1746-MONOMER; -.
DR   Proteomes; UP000000531; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0010501; P:RNA secondary structure unwinding; IEA:InterPro.
DR   HAMAP; MF_01493; DEAD_helicase_CshA; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR030880; DEAD_helicase_CshA.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome; RNA-binding; Stress response.
FT   CHAIN         1    509       DEAD-box ATP-dependent RNA helicase CshA.
FT                                /FTId=PRO_0000284828.
FT   DOMAIN       33    203       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01493}.
FT   DOMAIN      214    375       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_01493}.
FT   NP_BIND      46     53       ATP. {ECO:0000255|HAMAP-Rule:MF_01493}.
FT   MOTIF         2     30       Q motif.
FT   MOTIF       150    153       DEAD box.
SQ   SEQUENCE   509 AA;  57852 MW;  1E522360106F7D28 CRC64;
     MQNFKELGIS DKTVQTLEAM GFKEPTPIQK DSIPYALEGD DILGQAQTGT GKTGAFGIPL
     IEKVVGQQGV QSLILAPTRE LAMQVAEQLR EFSKGQKVQV VTVFGGMPIE RQIKALKRGP
     QIVVGTPGRV IDHLNRRTLK TQGIHTLILD EADEMMNMGF IDDMRFIMDK IPAEQRQTML
     FSATMPKAIQ ELVQQFMKAP KIIKTMNNEM SDPQIDEYYT IVKELEKFDT FTNFLDVHQP
     ELAIVFGRTK RRVDELTSAL LSKGYKAEGL HGDITQAKRL EVLKKFKNDQ IDILVATDVA
     ARGLDISGVS HVYNFDIPQD TESYTHRIGR TGRAGKEGIA VTFVNPIEMD YIRQIEDVNN
     RRMKALRPPH RKEVLKARED DIKDRVQNWM SRENEPRLQR ISSELLKEYD STELVASLLQ
     ELVEANDEVE VQLTFEKPLA RKNRSSKGGS RRSNHKRGNG KFDNKNRRSK GSKGQSSKKK
     NQKKFDRRDK QQKSGNQSLK GRTFADHQK
//
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