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Database: UniProt
Entry: Q5JK84
LinkDB: Q5JK84
Original site: Q5JK84 
ID   RH15_ORYSJ              Reviewed;         432 AA.
AC   Q5JK84; B7F820;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   16-OCT-2019, entry version 96.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 15;
DE            EC=3.6.4.13;
DE   AltName: Full=API5-interacting protein 1 {ECO:0000303|PubMed:21467577};
GN   Name=AIP2 {ECO:0000303|PubMed:21467577};
GN   OrderedLocusNames=Os01g0550000, LOC_Os01g36920;
GN   ORFNames=B1156H12.21-1, OSJNBa0024F24.6-1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M.,
RA   Okamoto M., Ando T., Aoki H., Arita K., Hamada M., Harada C.,
RA   Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M.,
RA   Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K.,
RA   Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I.,
RA   Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y.,
RA   Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M.,
RA   Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M.,
RA   Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K.,
RA   Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K.,
RA   Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y.,
RA   Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   FUNCTION, SUBUNIT, INTERACTION WITH API5, AND SUBCELLULAR LOCATION.
RX   PubMed=21467577; DOI=10.1105/tpc.110.082636;
RA   Li X., Gao X., Wei Y., Deng L., Ouyang Y., Chen G., Li X., Zhang Q.,
RA   Wu C.;
RT   "Rice APOPTOSIS INHIBITOR5 coupled with two DEAD-box adenosine 5'-
RT   triphosphate-dependent RNA helicases regulates tapetum degeneration.";
RL   Plant Cell 23:1416-1434(2011).
CC   -!- FUNCTION: ATP-binding RNA helicase involved in pre-mRNA splicing.
CC       Required for the export of mRNA out of the nucleus (By
CC       similarity). Required for tapetal programmed cell death (PCD) and
CC       degeneration during anther development. Forms dimer with AIP1 and
CC       binds the promoter region of the cysteine protease CP1. Can
CC       complement the yeast RNA helicase SUB2. Plants silencing AIP1 and
CC       AIP2 are male sterile (PubMed:21467577).
CC       {ECO:0000250|UniProtKB:Q07478, ECO:0000269|PubMed:21467577}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Homodimer and heterodimer with AIP1 (PubMed:21467577).
CC       Interacts with API5 (PubMed:21467577).
CC       {ECO:0000269|PubMed:21467577}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21467577}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DECD
CC       subfamily. {ECO:0000305}.
DR   EMBL; AP004225; BAD88055.1; -; Genomic_DNA.
DR   EMBL; AP004258; BAD88115.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF05212.1; -; Genomic_DNA.
DR   EMBL; AP014957; BAS72635.1; -; Genomic_DNA.
DR   EMBL; AK122050; BAH00768.1; -; mRNA.
DR   RefSeq; XP_015621815.1; XM_015766329.1.
DR   SMR; Q5JK84; -.
DR   STRING; 4530.OS01T0550000-01; -.
DR   PaxDb; Q5JK84; -.
DR   PRIDE; Q5JK84; -.
DR   EnsemblPlants; Os01t0550000-01; Os01t0550000-01; Os01g0550000.
DR   GeneID; 4326193; -.
DR   Gramene; Os01t0550000-01; Os01t0550000-01; Os01g0550000.
DR   KEGG; osa:4326193; -.
DR   eggNOG; KOG0329; Eukaryota.
DR   eggNOG; COG0513; LUCA.
DR   HOGENOM; HOG000268797; -.
DR   InParanoid; Q5JK84; -.
DR   KO; K12812; -.
DR   OMA; CGYQKMT; -.
DR   OrthoDB; 779000at2759; -.
DR   Proteomes; UP000059680; Chromosome 1.
DR   ExpressionAtlas; Q5JK84; baseline and differential.
DR   Genevisible; Q5JK84; OS.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0000346; C:transcription export complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0048653; P:anther development; IMP:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Coiled coil; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; RNA-binding.
FT   CHAIN         1    432       DEAD-box ATP-dependent RNA helicase 15.
FT                                /FTId=PRO_0000282496.
FT   DOMAIN       82    255       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      283    428       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      95    102       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   COILED        3     28       {ECO:0000255}.
FT   MOTIF        51     79       Q motif.
FT   MOTIF       202    205       DEAD box.
SQ   SEQUENCE   432 AA;  48567 MW;  EE00942821DC1F2F CRC64;
     MGEAEVKDNE VYEEDLVDYE EEVENGADGG AAAANASADV VKKGYVGIHS SGFRDFLLKP
     ELLRAIQDCG FEHPSEVQHE CIPQAILGMD VICQAKSGMG KTAVFVLSSL QQIDPVAGQV
     GALVLCHTRE LAYQICHEFE RFSKYLPEVK VAVFYGGVHI KKHKDLLKND CPHIVVGTPG
     RILALAREKD LSLKNVRHFI LDECDKMLDS LDMRRDVQEI FKMTPHDKQV MMFSATLSKE
     IRPVCKKFMQ DPMEIYVDDE AKLTLHGLVQ HYIKLSEAEK NRKLNDLLDA LDFNQVVIFV
     KSVSRAAELN KLLCECNFPA ISIHSGMTQE ERLTRYKNFK EGHKRILVAT DLVGRGIDIE
     RVNIVINYDM PDSADSYLHR VGRAGRFGTK GLAITFVSSA SDSDVLNQVQ ERFEVDIKEL
     PEQIDTSTYM PS
//
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