ID Q5K924_CRYNJ Unreviewed; 606 AA.
AC Q5K924; Q55LP9;
DT 15-FEB-2005, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2005, sequence version 1.
DT 27-MAR-2024, entry version 137.
DE RecName: Full=alpha-amylase {ECO:0000256|ARBA:ARBA00012595};
DE EC=3.2.1.1 {ECO:0000256|ARBA:ARBA00012595};
GN OrderedLocusNames=CNH03660 {ECO:0000313|EMBL:AAW45221.1}, CNL03660
GN {ECO:0000313|EMBL:AAW46412.1};
OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS MYA-565) (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=214684 {ECO:0000313|EMBL:AAW46412.1, ECO:0000313|Proteomes:UP000002149};
RN [1] {ECO:0000313|EMBL:AAW46412.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JEC21 {ECO:0000313|EMBL:AAW46412.1};
RA Loftus B., Amedeo P., Roncaglia P., Vamathevan J., Utterback T.,
RA Van Aken S., Fraser C.;
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AAW46412.1, ECO:0000313|Proteomes:UP000002149}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEC21 {ECO:0000313|EMBL:AAW46412.1}, and JEC21 / ATCC MYA-565
RC {ECO:0000313|Proteomes:UP000002149};
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J., Koo H.L., Krzywinski M.I., Kwon-Chung J.K.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
RN [3] {ECO:0000313|EMBL:AAW46412.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JEC21 {ECO:0000313|EMBL:AAW46412.1};
RA Janbon G., Paulet D., Chon C.C., Mornico D.;
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
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DR EMBL; AE017348; AAW45221.1; -; Genomic_DNA.
DR EMBL; AE017352; AAW46412.1; -; Genomic_DNA.
DR RefSeq; XP_567929.1; XM_567929.1.
DR RefSeq; XP_572528.1; XM_572528.1.
DR AlphaFoldDB; Q5K924; -.
DR STRING; 214684.Q5K924; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR PaxDb; 214684-Q5K924; -.
DR EnsemblFungi; AAW45221; AAW45221; CNH03660.
DR EnsemblFungi; AAW46412; AAW46412; CNL03660.
DR GeneID; 3254804; -.
DR GeneID; 3259116; -.
DR KEGG; cne:CNH03660; -.
DR KEGG; cne:CNL03660; -.
DR VEuPathDB; FungiDB:CNH03660; -.
DR VEuPathDB; FungiDB:CNL03660; -.
DR eggNOG; KOG0471; Eukaryota.
DR HOGENOM; CLU_006462_7_2_1; -.
DR OMA; TEANYCG; -.
DR OrthoDB; 3249969at2759; -.
DR Proteomes; UP000002149; Chromosome 12.
DR Proteomes; UP000002149; Chromosome 8.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd11319; AmyAc_euk_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR015340; A_amylase_C_dom.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10357:SF215; ALPHA-AMYLASE 1; 1.
DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR Pfam; PF09260; A_amylase_dom_C; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000002149};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..606
FT /note="alpha-amylase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5014309877"
FT DOMAIN 32..397
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 606 AA; 64351 MW; BB6335C9C21D4623 CRC64;
MPVLSKLISL LPLLAIAQGA TSDEWRSRSI YQLITDRFAP PSDDTTCPLG ATNYCGGTWQ
TIISKLDYIQ NMGFDAIWIS PTALNIEGNT KYGEAYHGYW TADPTKLNPH FGQASDLKAL
SVAVHDRGMY LMVDIAINAL AATSYSLDAS ALASDADGTL LFKDPSDFHT RCDISWGNHT
SEQVCWLVTG DDNGDVALLD LKTESDSVAS VLKDWVGGYV TEYGIDGFRI DASKHMSKEF
QHDFCEAAGT FCMGEVAGDN TEYAGEYQSA GGIDSVCGFG LMYGFVNVFT GGDKMSTLEY
YINLAASSYP DPTVIGTFLD NQDLPRFNSL TSDASLVYNA IVGMFMYGGM PIVYYGLEQD
ISDGPTDPQN REALWNYNNY ATDGVTFGRI TNLNNIRNRL GGVGELYNAV ATVLAIQDQD
IALQREEALI VLTNRGQSGT GTWAIKNTQF GGSVSVIDLL SCSTSTTDSD GSLTVTWTSG
QPFVFVASDV ADKAGLCGSL SNSTNATTVS SSSSASTSID TATSSASISG NLAVITDGSS
SPSFSATSSA LSSSISWYTP TSAASSVSSS ELVVVVAAVS TASTAVTSSG SICRRSKKRA
MSKRQL
//
