ID Q5KMI8_CRYNJ Unreviewed; 937 AA.
AC Q5KMI8; Q55XE5;
DT 15-FEB-2005, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 2.
DT 27-MAR-2024, entry version 131.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364117};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU364117};
GN OrderedLocusNames=CNB01550 {ECO:0000313|EMBL:AAW41518.2};
OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS MYA-565) (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=214684 {ECO:0000313|EMBL:AAW41518.2, ECO:0000313|Proteomes:UP000002149};
RN [1] {ECO:0000313|EMBL:AAW41518.2, ECO:0000313|Proteomes:UP000002149}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEC21 / ATCC MYA-565 {ECO:0000313|Proteomes:UP000002149};
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J., Koo H.L., Krzywinski M.I., Kwon-Chung J.K.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU364117};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU364117}.
CC -!- SIMILARITY: Belongs to the helicase family. RecQ subfamily.
CC {ECO:0000256|ARBA:ARBA00005446, ECO:0000256|RuleBase:RU364117}.
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DR EMBL; AE017342; AAW41518.2; -; Genomic_DNA.
DR RefSeq; XP_568825.1; XM_568825.1.
DR AlphaFoldDB; Q5KMI8; -.
DR STRING; 214684.Q5KMI8; -.
DR PaxDb; 214684-Q5KMI8; -.
DR EnsemblFungi; AAW41518; AAW41518; CNB01550.
DR VEuPathDB; FungiDB:CNB01550; -.
DR eggNOG; KOG0351; Eukaryota.
DR HOGENOM; CLU_001103_12_0_1; -.
DR InParanoid; Q5KMI8; -.
DR OrthoDB; 5474026at2759; -.
DR Proteomes; UP000002149; Chromosome 2.
DR GO; GO:0005694; C:chromosome; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0009378; F:four-way junction helicase activity; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR CDD; cd17920; DEXHc_RecQ; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR004589; DNA_helicase_ATP-dep_RecQ.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR032284; RecQ_Zn-bd.
DR NCBIfam; TIGR00614; recQ_fam; 1.
DR PANTHER; PTHR13710:SF105; BLOOM SYNDROME PROTEIN; 1.
DR PANTHER; PTHR13710; DNA HELICASE RECQ FAMILY MEMBER; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF16124; RecQ_Zn_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU364117};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU364117};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU364117};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU364117}; Nucleus {ECO:0000256|RuleBase:RU364117};
KW Reference proteome {ECO:0000313|Proteomes:UP000002149}.
FT DOMAIN 69..244
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 282..444
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 519..562
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 578..629
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 657..697
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 536..562
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 587..626
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 669..686
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 937 AA; 106153 MW; BB1A5B575A1B7A75 CRC64;
MLAFPSSTPA ERPYTNLVNS TQFARNNFRP TKREWRRTMS PQETPSALQR NLKHHWGYND
FRHPQLEICT DALRGCDLIV VAPTGLGKSL CFQLPAITIE HGVTIVVSPL KALMSDQVKD
LTSRGIKAVQ LNEYTTLAEH NEVRRQMRMG HPEIRLLYVT PEMLLSDKHR STFDTAYAQK
QVARLVVDEA HVITEWGTSF RGKYRELGKF RERYYDIPIT ALTASATKEV RHDIIQTLRI
RKGYGQWVMP FNRRNLFYEI RYQGRGSIEK EEMEVQKNPL DDIVDFIEKY RPEAAKRNRE
NGIFRICVTG IVYCRTTAAC EEVAGFLSNR RIKAMPYYKN LSQTVKDQAL AGWKDGSIEC
IVATIAFGMG IDQANVRYVI HYQMPKTFEG YYQETGRAGR DGHISHCILY YSREDAKYLR
WLLEQEDAKQ KRIARFKSGD ACAETSSQTL NSFKALQHYM ESLGRCRHVG ICSYFGEKID
DKDPEIKAAY CQSMCDICKD NAKVRKAAMH LTDAIPVASA IAGREPTPIP DQDPKPEPLF
DPREGDDTDT DEHHDEFLDL HGPDPDIFEE GLAGTAPPIF QIADPRSSGS HAGNHQNTPL
VSSTNSHVQS SNTIPSSMSS KPPSVPLSRT PVLVRDLSSE RVAQPIRVRE IIHIETGGEP
EPQSLANKRR RAGQGSMLSS SPTSSMEFEE IEKEREREME KVRLRKEKER QEAMARERER
PIFSSNARLP DNVKFVQDSE EGTASELKLT REQRIKAERM LNSVKPVRGE GPYACYNAAP
PAARFRKASS ASDKAFKPPI LKSPTKVRCD LLTKSARDNA VLEISNSLKD SLGHGDLART
VLNSWGRLER GSKRVTVLQD VARAIERDFA AISREDPLGF ERRITEFRKA AKALRSSEVV
DAIAQGDIDL FDDGSPEVGH LKSLEKYMRT WKPEPSE
//
