GenomeNet

Database: UniProt
Entry: Q5L3C7_GEOKA
LinkDB: Q5L3C7_GEOKA
Original site: Q5L3C7_GEOKA 
ID   Q5L3C7_GEOKA            Unreviewed;       430 AA.
AC   Q5L3C7;
DT   01-FEB-2005, integrated into UniProtKB/TrEMBL.
DT   01-FEB-2005, sequence version 1.
DT   08-MAY-2019, entry version 118.
DE   RecName: Full=Phosphoribosylamine--glycine ligase {ECO:0000256|HAMAP-Rule:MF_00138};
DE            EC=6.3.4.13 {ECO:0000256|HAMAP-Rule:MF_00138};
DE   AltName: Full=GARS {ECO:0000256|HAMAP-Rule:MF_00138};
DE   AltName: Full=Glycinamide ribonucleotide synthetase {ECO:0000256|HAMAP-Rule:MF_00138};
DE   AltName: Full=Phosphoribosylglycinamide synthetase {ECO:0000256|HAMAP-Rule:MF_00138};
GN   Name=purD {ECO:0000256|HAMAP-Rule:MF_00138};
GN   OrderedLocusNames=GK0268 {ECO:0000313|EMBL:BAD74553.1};
OS   Geobacillus kaustophilus (strain HTA426).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC   Geobacillus thermoleovorans group.
OX   NCBI_TaxID=235909 {ECO:0000313|EMBL:BAD74553.1, ECO:0000313|Proteomes:UP000001172};
RN   [1] {ECO:0000313|EMBL:BAD74553.1, ECO:0000313|Proteomes:UP000001172}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HTA426 {ECO:0000313|EMBL:BAD74553.1,
RC   ECO:0000313|Proteomes:UP000001172};
RX   PubMed=15576355; DOI=10.1093/nar/gkh970;
RA   Takami H., Takaki Y., Chee G.J., Nishi S., Shimamura S., Suzuki H.,
RA   Matsui S., Uchiyama I.;
RT   "Thermoadaptation trait revealed by the genome sequence of
RT   thermophilic Geobacillus kaustophilus.";
RL   Nucleic Acids Res. 32:6292-6303(2004).
RN   [2] {ECO:0000213|PDB:2YRW, ECO:0000213|PDB:2YRX, ECO:0000213|PDB:2YS6}
RP   X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH AMP.
RX   PubMed=20716513; DOI=10.1093/jb/mvq088;
RA   Sampei G., Baba S., Kanagawa M., Yanai H., Ishii T., Kawai H.,
RA   Fukai Y., Ebihara A., Nakagawa N., Kawai G.;
RT   "Crystal structures of glycinamide ribonucleotide synthetase, PurD,
RT   from thermophilic eubacteria.";
RL   J. Biochem. 148:429-438(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-phospho-D-ribosylamine + ATP + glycine = ADP + H(+) +
CC         N(1)-(5-phospho-D-ribosyl)glycinamide + phosphate;
CC         Xref=Rhea:RHEA:17453, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57305, ChEBI:CHEBI:58089,
CC         ChEBI:CHEBI:58457, ChEBI:CHEBI:456216; EC=6.3.4.13;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00138};
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-
CC       ribose 1-diphosphate: step 2/2. {ECO:0000256|HAMAP-Rule:MF_00138}.
CC   -!- SIMILARITY: Belongs to the GARS family. {ECO:0000256|HAMAP-
CC       Rule:MF_00138}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; BA000043; BAD74553.1; -; Genomic_DNA.
DR   RefSeq; WP_011229777.1; NC_006510.1.
DR   PDB; 2YRW; X-ray; 2.20 A; A=1-430.
DR   PDB; 2YRX; X-ray; 1.90 A; A=1-430.
DR   PDB; 2YS6; X-ray; 2.21 A; A=1-430.
DR   PDB; 2YS7; X-ray; 2.21 A; A=1-430.
DR   PDBsum; 2YRW; -.
DR   PDBsum; 2YRX; -.
DR   PDBsum; 2YS6; -.
DR   PDBsum; 2YS7; -.
DR   SMR; Q5L3C7; -.
DR   STRING; 235909.GK0268; -.
DR   EnsemblBacteria; BAD74553; BAD74553; GK0268.
DR   GeneID; 32062269; -.
DR   KEGG; gka:GK0268; -.
DR   PATRIC; fig|235909.7.peg.336; -.
DR   eggNOG; ENOG4105C12; Bacteria.
DR   eggNOG; COG0151; LUCA.
DR   HOGENOM; HOG000033464; -.
DR   KO; K01945; -.
DR   OMA; KATVCKY; -.
DR   BRENDA; 6.3.4.13; 8138.
DR   UniPathway; UPA00074; UER00125.
DR   EvolutionaryTrace; Q5L3C7; -.
DR   Proteomes; UP000001172; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.90.600.10; -; 1.
DR   HAMAP; MF_00138; GARS; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR016185; PreATP-grasp_dom_sf.
DR   InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR   InterPro; IPR000115; PRibGlycinamide_synth.
DR   InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR   InterPro; IPR037123; PRibGlycinamide_synth_C_sf.
DR   InterPro; IPR020559; PRibGlycinamide_synth_CS.
DR   InterPro; IPR020562; PRibGlycinamide_synth_N.
DR   InterPro; IPR011054; Rudment_hybrid_motif.
DR   Pfam; PF01071; GARS_A; 1.
DR   Pfam; PF02843; GARS_C; 1.
DR   Pfam; PF02844; GARS_N; 1.
DR   SMART; SM01210; GARS_C; 1.
DR   SUPFAM; SSF51246; SSF51246; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR00877; purD; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00184; GARS; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0000213|PDB:2YRW, ECO:0000213|PDB:2YRX,
KW   ECO:0000213|PDB:2YS6, ECO:0000213|PDB:2YS7};
KW   ATP-binding {ECO:0000256|PROSITE-ProRule:PRU00409};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001172};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_00138, ECO:0000313|EMBL:BAD74553.1};
KW   Nucleotide-binding {ECO:0000213|PDB:2YRX, ECO:0000213|PDB:2YS6,
KW   ECO:0000256|PROSITE-ProRule:PRU00409};
KW   Purine biosynthesis {ECO:0000256|HAMAP-Rule:MF_00138};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001172}.
FT   DOMAIN      107    313       ATP-grasp. {ECO:0000259|PROSITE:PS50975}.
FT   NP_BIND     186    188       AMP. {ECO:0000213|PDB:2YRX}.
FT   BINDING     192    192       AMP. {ECO:0000213|PDB:2YRX,
FT                                ECO:0000213|PDB:2YS6}.
SQ   SEQUENCE   430 AA;  45734 MW;  19139946D7A05A18 CRC64;
     MNVLVIGRGG REHAIAWKAA QSPLVGKLYV APGNPGIADV AELVHIDELD IEALVQFAKQ
     QAIDLTIVGP EAPLASGIVD RFMAEGLRIF GPSQRAALIE GSKAFAKELM KKYGIPTADH
     AAFTSYEEAK AYIEQKGAPI VIKADGLAAG KGVTVAQTVE EALAAAKAAL VDGQFGTAGS
     QVVIEEYLEG EEFSFMAFVN GEKVYPLAIA QDHKRAYDGD EGPNTGGMGA YSPVPQISDE
     MMDAALEAIL RPAAKALAAE GRPFLGVLYA GLMATANGPK VIEFNARFGD PEAQVVLPRL
     KTDLVEAVLA VMDGKELELE WTDEAVLGVV LAAKGYPGAY ERGAEIRGLD RISPDALLFH
     AGTKREGGAW YTNGGRVLLL AAKGETLAKA KEKAYEQLAA IDCDGLFYRR DIGRRAIERA
     SAAYTRMKGR
//
DBGET integrated database retrieval system