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Database: UniProt
Entry: Q5NFN4
LinkDB: Q5NFN4
Original site: Q5NFN4 
ID   UVRB_FRATT              Reviewed;         668 AA.
AC   Q5NFN4;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   13-NOV-2019, entry version 94.
DE   RecName: Full=UvrABC system protein B {ECO:0000255|HAMAP-Rule:MF_00204};
DE            Short=Protein UvrB {ECO:0000255|HAMAP-Rule:MF_00204};
DE   AltName: Full=Excinuclease ABC subunit B {ECO:0000255|HAMAP-Rule:MF_00204};
GN   Name=uvrB {ECO:0000255|HAMAP-Rule:MF_00204};
GN   OrderedLocusNames=FTT_1199c;
OS   Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC   Francisellaceae; Francisella.
OX   NCBI_TaxID=177416;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCHU S4 / Schu 4;
RX   PubMed=15640799; DOI=10.1038/ng1499;
RA   Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M.,
RA   Fuxelius H.-H., Garcia E., Haelltorp G., Johansson D., Isherwood K.E.,
RA   Karp P.D., Larsson E., Liu Y., Michell S., Prior J., Prior R.,
RA   Malfatti S., Sjoestedt A., Svensson K., Thompson N., Vergez L.,
RA   Wagg J.K., Wren B.W., Lindler L.E., Andersson S.G.E., Forsman M.,
RA   Titball R.W.;
RT   "The complete genome sequence of Francisella tularensis, the causative
RT   agent of tularemia.";
RL   Nat. Genet. 37:153-159(2005).
CC   -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC       processing of DNA lesions. A damage recognition complex composed
CC       of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon
CC       binding of the UvrA(2)B(2) complex to a putative damaged site, the
CC       DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP
CC       binding by UvrB and probably causes local melting of the DNA
CC       helix, facilitating insertion of UvrB beta-hairpin between the DNA
CC       strands. Then UvrB probes one DNA strand for the presence of a
CC       lesion. If a lesion is found the UvrA subunits dissociate and the
CC       UvrB-DNA preincision complex is formed. This complex is
CC       subsequently bound by UvrC and the second UvrB is released. If no
CC       lesion is found, the DNA wraps around the other UvrB subunit that
CC       will check the other stand for damage. {ECO:0000255|HAMAP-
CC       Rule:MF_00204}.
CC   -!- SUBUNIT: Forms a heterotetramer with UvrA during the search for
CC       lesions. Interacts with UvrC in an incision complex.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- DOMAIN: The beta-hairpin motif is involved in DNA binding.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SIMILARITY: Belongs to the UvrB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00204}.
DR   EMBL; AJ749949; CAG45832.1; -; Genomic_DNA.
DR   RefSeq; WP_003021449.1; NC_006570.2.
DR   RefSeq; YP_170158.1; NC_006570.2.
DR   SMR; Q5NFN4; -.
DR   IntAct; Q5NFN4; 13.
DR   STRING; 177416.FTT_1199c; -.
DR   PRIDE; Q5NFN4; -.
DR   DNASU; 3191920; -.
DR   EnsemblBacteria; CAG45832; CAG45832; FTT_1199c.
DR   GeneID; 3191920; -.
DR   KEGG; ftu:FTT_1199c; -.
DR   eggNOG; ENOG4105CCW; Bacteria.
DR   eggNOG; COG0556; LUCA.
DR   HOGENOM; HOG000073580; -.
DR   KO; K03702; -.
DR   OMA; RYMHSEI; -.
DR   BioCyc; FTUL177416:G1G1C-1336-MONOMER; -.
DR   Proteomes; UP000001174; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATPase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00204; UvrB; 1.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001943; UVR_dom.
DR   InterPro; IPR036876; UVR_dom_sf.
DR   InterPro; IPR004807; UvrB.
DR   InterPro; IPR041471; UvrB_inter.
DR   InterPro; IPR024759; UvrB_YAD/RRR_dom.
DR   PANTHER; PTHR24029; PTHR24029; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   Pfam; PF02151; UVR; 1.
DR   Pfam; PF12344; UvrB; 1.
DR   Pfam; PF17757; UvrB_inter; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46600; SSF46600; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00631; uvrb; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 2.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50151; UVR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA excision;
KW   DNA repair; Excision nuclease; Nucleotide-binding; Reference proteome;
KW   SOS response.
FT   CHAIN         1    668       UvrABC system protein B.
FT                                /FTId=PRO_0000227314.
FT   DOMAIN       25    413       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00204}.
FT   DOMAIN      429    595       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_00204}.
FT   DOMAIN      629    664       UVR. {ECO:0000255|HAMAP-Rule:MF_00204}.
FT   NP_BIND      38     45       ATP. {ECO:0000255|HAMAP-Rule:MF_00204}.
FT   MOTIF        91    114       Beta-hairpin.
SQ   SEQUENCE   668 AA;  76222 MW;  E40F667EC452AE54 CRC64;
     MNKFNLVTKY APAGDQPQAI QSLVNGINTG LQHQVLLGVT GSGKTYTMAN VIQQTQKPCL
     ILAHNKTLAA QLYSEFKQYF PDNAVEYFVS YYDYYQPEAY IAASDTYIEK DSSVNEHIEQ
     MRLSATKAIL ERNDVIIVAT VSAIYGLGDP EQYMQMLLHL KVGEELGLKK AQTKLVEMQY
     SRNDMDFSRG SFRVRGEVLD IFPADSEKDA IRVEFFDDEI EAISVIDSLT SKKLKSLHRA
     TIFPSTHYVA SKERKEIVIE DIKKELKERV KYFEKEGKLL EAQRIEQRTK YDIEMIQELG
     YCTGIENYSR LLSGRAPGYP PPTLIDYLPE NALVIVDESH VTLPQFGGMY KGDLSRKSNL
     VNYGFRLPSA LDNRPLKFNE FESLLPQTIY VSATPANYEL EKSQNTVQQV IRPTGLLDPE
     VFVRPVAIQV EDALSEINKA IAKEERVLIT TLTKKMVENL TEYLSEHGVN VRYLHSDIDT
     VERVQIIHDL RHGVFDVLVG INLLREGLDM PEVGVLLIFD ADKEGFLRSE KSLIQTIGRV
     ARNQNGRAIL YADVVTKSMQ KAMDETLRRR KLQDEYNQKH NIVPKTIIKN IDDMLDSSPE
     MQKRAYKNNL RLKVDDVDVS AILGMTEATK VIKALEKRMR AYAKELEFEK ATTIRDKITE
     VKQKFINL
//
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