ID Q5YPP4_NOCFA Unreviewed; 842 AA.
AC Q5YPP4;
DT 23-NOV-2004, integrated into UniProtKB/TrEMBL.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 122.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN Name=uvrD {ECO:0000313|EMBL:BAD59847.1};
GN OrderedLocusNames=NFA_49950 {ECO:0000313|EMBL:BAD59847.1};
OS Nocardia farcinica (strain IFM 10152).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=247156 {ECO:0000313|EMBL:BAD59847.1, ECO:0000313|Proteomes:UP000006820};
RN [1] {ECO:0000313|EMBL:BAD59847.1, ECO:0000313|Proteomes:UP000006820}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 10152 {ECO:0000313|EMBL:BAD59847.1,
RC ECO:0000313|Proteomes:UP000006820};
RX PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA Shiba T., Hattori M.;
RT "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618,
CC ECO:0000256|RuleBase:RU364053};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP006618; BAD59847.1; -; Genomic_DNA.
DR RefSeq; WP_011211530.1; NC_006361.1.
DR AlphaFoldDB; Q5YPP4; -.
DR STRING; 247156.NFA_49950; -.
DR GeneID; 61135578; -.
DR KEGG; nfa:NFA_49950; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_2_11; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000006820; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 2.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000006820}.
FT DOMAIN 52..347
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 348..652
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 739..789
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..44
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 739..773
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 73..80
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 842 AA; 92977 MW; EFBEFCE3221EE371 CRC64;
MDMTVVPQTT APQTTPAGRS LAEQAQQRAD DQARRRADRD RQRAEQVERL LDGLNPQQRA
AVVHAGSPLL IVAGAGSGKT AVLTRRIAYL LAARDVSPGQ ILAITFTNKA AAEMRERVTG
LVGPRAATMW VSTFHSSCVR ILRMQAALLP GLNSNFSIYD ADDSRRLLTM ISRDLDIDTK
KYSARLLVTA ISNLKNELIG PEQASADAES DESELPGLVA RVYTEYQRRL RAANALDFDD
LIGETVALLQ NHPQVAEYYR RRFRHVLVDE YQDTNHAQYV LVRELVGHHS AADDTEDAVP
PSELCVVGDA DQSIYAFRGA TIRNIEEFER DFPDAETILL EQNYRSTQNI LSAANAVIAR
NEGRREKRLW TDSGEGDLIV GYVADNEHDE ASFVAREIDR LVDQGEANYG DVAVFYRTNN
NSRALEEIFI RMGLPYKVVG GVRFYERKEV RDIVAYLRVL ENPDDAVSLR RILNTPRRGI
GDRAEACVAV HAEQRGIGFA AALRDAAEGK VALLNTRAQR AIAGFLDLLE EIRAAGEQAD
ADFPDVGNVV EAVLDRTGYR AELEASDDPQ DGARLDNLNE LVSVAREFSS EAYNNAVAAR
EEGILPEQAE GEPDPGSLAA FLERVSLVAD TDQIPDEGAG VVTMMTLHTA KGLEFPVVFV
TGWEDGQFPH MRALGDPTEL AEERRLAYVG ITRARQRLYL TRAVVRSGWG QPVSNPESRF
LQEIPDHLIE WRRLEPSPAT RGIRKRGGDE GLERDWTRGD GWSEPRPGVR DRGPRRPAPG
GASRNNTNLV LAVGDRVSDD KYGLGRVVAA DGFGPLATVT IDFGTAGKIR LIPQYSRTLV
KL
//
