ID Q5YR02_NOCFA Unreviewed; 1184 AA.
AC Q5YR02;
DT 23-NOV-2004, integrated into UniProtKB/TrEMBL.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 127.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN OrderedLocusNames=NFA_45380 {ECO:0000313|EMBL:BAD59389.1};
OS Nocardia farcinica (strain IFM 10152).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=247156 {ECO:0000313|EMBL:BAD59389.1, ECO:0000313|Proteomes:UP000006820};
RN [1] {ECO:0000313|EMBL:BAD59389.1, ECO:0000313|Proteomes:UP000006820}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 10152 {ECO:0000313|EMBL:BAD59389.1,
RC ECO:0000313|Proteomes:UP000006820};
RX PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA Shiba T., Hattori M.;
RT "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
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DR EMBL; AP006618; BAD59389.1; -; Genomic_DNA.
DR RefSeq; WP_011211073.1; NC_006361.1.
DR AlphaFoldDB; Q5YR02; -.
DR STRING; 247156.NFA_45380; -.
DR GeneID; 61135145; -.
DR KEGG; nfa:NFA_45380; -.
DR eggNOG; COG0210; Bacteria.
DR eggNOG; COG2887; Bacteria.
DR HOGENOM; CLU_003630_1_1_11; -.
DR OrthoDB; 4812256at2; -.
DR Proteomes; UP000006820; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000006820}.
FT DOMAIN 15..367
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 368..691
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 840..863
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 882..946
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 886..924
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1184 AA; 125239 MW; 8AE002A5659A893F CRC64;
MTVSPHRIAD ALGLPPPTDE QAAVIAAPPG PTLVVAGAGA GKTETMAARV VWMVANRLVL
PEQVLGLTFT RKAAQQLTAR IRTRLARLAG SALLRELDSG GELRAQLAGA EPEISTYHSY
AGRLLSEHGL LLPVEPSATL LTETQLWQLA HQVVRNWDGD LDTERTPVSV TEAVLALSGQ
LAEHLVEPEE LAEAHAELEK LINTLPAGPR QRGGPSKALR DIVRVQHERV ALLPLVRALN
DALRRRGALD FGAQMSLAAR LAAEHPEVAE AERGRFRLVL LDEYQDTGHA QRVLLSALFG
GAAGDPEGTA GGRAPRPAVT AVGDPMQSIY GWRGASAANL PRFATDFPCA PGAPAPILPL
LTSWRNPPEA LALANLVAEP LRQAAREAGG ATVDALRAKP GAEPGVVALA LTETVVDERG
WVAERIAAEW AARRAAGEPP PTSAVLVRRN ADAAPLAEAL REQGLPVEIV GLGGLLATPE
VADIVATLRL IAEPAAGSAA LRVLTGARWR IGVADLAALS RRARELSVVR TDTAVGAEIT
DGAALEAALR EVTPEPVEQA GLADAIADPG PAENYSAAGY QRIEALGREL AALRERSGQP
LTELVADVER TIGVGVETQA RRAVLGAGAG REHLDAFAEV VAGYAADPGA SLGGLLAFLT
AAESVENGLE PGEVEVAKDR VQVLTVHAAK GLEWEIVAVP HVVRRVFPSG TAGGTWLGAL
AELPTALRGD RVAEDAAEGV PVLDLTDLYD RADLERALAA HKAALERRRI DEERRLFYVA
LTRTERVLLV SAHHWAETGD PKGPSDFLLE LKSAAETPGG PAHGAVEIAR WDDPPPVDAV
NPFADNPATA EWPRDPLGPR RDPVEQGAAL VRAALAELRA PAGPRAPAVP HAPAVPRPAP
RHTPTVPPPT DTDGPLPSAP NDDVPQPQSP CLDPVVTDVP TDPDDPEGWA ADVDALLAEH
EATVRAAREV ELPGQLPATA LVELRADPAK LARRLRRPLP YPPNPLARRG TAFHAWVQRW
FSGTRLLGFD ELPGAGEPDP GEAAADAELA RMQDAFLNSA WAHRSPIDVE VPFETSIAGT
VIRGRMDAVF AEPDGRWVVV DWKTGAEPDP GDEPAVAMQL AIYRLAWARL MAARTGGDEQ
AMLHRVDAAF HYVRSGRTIA PADLPGPDEL AELIRAAAPP REGE
//
