ID EFMT1_CHICK Reviewed; 213 AA.
AC Q5ZKT6;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 117.
DE RecName: Full=EEF1A lysine methyltransferase 1 {ECO:0000255|HAMAP-Rule:MF_03187};
DE EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_03187};
DE AltName: Full=N(6)-adenine-specific DNA methyltransferase 2 {ECO:0000255|HAMAP-Rule:MF_03187};
DE AltName: Full=Protein-lysine N-methyltransferase N6AMT2 {ECO:0000255|HAMAP-Rule:MF_03187};
GN Name=EEF1AKMT1 {ECO:0000255|HAMAP-Rule:MF_03187};
GN Synonyms=N6AMT2 {ECO:0000255|HAMAP-Rule:MF_03187}; ORFNames=RCJMB04_9d5;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
CC -!- FUNCTION: Protein-lysine methyltransferase that selectively catalyzes
CC the trimethylation of EEF1A at 'Lys-79'. {ECO:0000255|HAMAP-
CC Rule:MF_03187}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H(+) +
CC N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:54192, Rhea:RHEA-COMP:9752, Rhea:RHEA-
CC COMP:13826, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:61961;
CC Evidence={ECO:0000250|UniProtKB:Q8WVE0};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54193;
CC Evidence={ECO:0000250|UniProtKB:Q8WVE0};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03187}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. EFM5 family. {ECO:0000255|HAMAP-Rule:MF_03187}.
CC -!- CAUTION: Was originally thought to be an N(6)-adenine-specific DNA
CC methyltransferase based on primary sequence and predicted secondary
CC structure. {ECO:0000255|HAMAP-Rule:MF_03187}.
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DR EMBL; AJ719998; CAG31657.1; -; mRNA.
DR RefSeq; NP_001006277.1; NM_001006277.1.
DR RefSeq; XP_015133532.1; XM_015278046.1.
DR AlphaFoldDB; Q5ZKT6; -.
DR SMR; Q5ZKT6; -.
DR STRING; 9031.ENSGALP00000074055; -.
DR PaxDb; 9031-ENSGALP00000027624; -.
DR Ensembl; ENSGALT00000101742; ENSGALP00000074055; ENSGALG00000017133.
DR Ensembl; ENSGALT00010013193.1; ENSGALP00010007755.1; ENSGALG00010005514.1.
DR Ensembl; ENSGALT00015041842; ENSGALP00015024405; ENSGALG00015017131.
DR GeneID; 418951; -.
DR KEGG; gga:418951; -.
DR CTD; 221143; -.
DR VEuPathDB; HostDB:geneid_418951; -.
DR eggNOG; KOG3350; Eukaryota.
DR GeneTree; ENSGT00390000016366; -.
DR HOGENOM; CLU_074410_2_1_1; -.
DR InParanoid; Q5ZKT6; -.
DR OMA; CNFRPEH; -.
DR OrthoDB; 6014at2759; -.
DR PhylomeDB; Q5ZKT6; -.
DR TreeFam; TF106153; -.
DR Reactome; R-GGA-8876725; Protein methylation.
DR PRO; PR:Q5ZKT6; -.
DR Proteomes; UP000000539; Chromosome 1.
DR Bgee; ENSGALG00000017133; Expressed in spermatid and 14 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042054; F:histone methyltransferase activity; IEA:RHEA.
DR GO; GO:0008168; F:methyltransferase activity; ISS:UniProtKB.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0016279; F:protein-lysine N-methyltransferase activity; ISS:UniProtKB.
DR GO; GO:0018022; P:peptidyl-lysine methylation; ISS:UniProtKB.
DR HAMAP; MF_03187; Methyltr_EFM5; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR019369; Efm5/EEF1AKMT1.
DR InterPro; IPR041370; Mlase_EEF1AKMT1/ZCCHC4.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR13200:SF0; EEF1A LYSINE METHYLTRANSFERASE 1; 1.
DR PANTHER; PTHR13200; UNCHARACTERIZED; 1.
DR Pfam; PF10237; N6-adenineMlase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Methyltransferase; Reference proteome; Transferase.
FT CHAIN 1..213
FT /note="EEF1A lysine methyltransferase 1"
FT /id="PRO_0000311296"
SQ SEQUENCE 213 AA; 24079 MW; BDDC0CAA4B2A1FA4 CRC64;
MDDDDDDIPQ LSSHTLAALQ EFYLEQQQRE GMKTSQGFNQ YSVGSIEEDW QLSQFWYSDE
TASCLANEAI VAAGKGGRIA CVSAPSVYQK LREQGGADFS VCILEYDRRF SVYGEEFIFY
DYNNPLNLPE HLLPHSFDIV VADPPYLSEE CLQKTAETIK YLTKGKILLC TGAIMEEQAA
KHLGVKICKF IPKHSRNLAN EFRCYVNYAS GLD
//
