ID NHRF1_CHICK Reviewed; 333 AA.
AC Q5ZM14;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 99.
DE RecName: Full=Na(+)/H(+) exchange regulatory cofactor NHE-RF1;
DE Short=NHERF-1;
DE AltName: Full=Ezrin-radixin-moesin-binding phosphoprotein 50;
DE Short=EBP50;
DE AltName: Full=Regulatory cofactor of Na(+)/H(+) exchanger;
DE AltName: Full=Sodium-hydrogen exchanger regulatory factor 1;
DE AltName: Full=Solute carrier family 9 isoform A3 regulatory factor 1;
GN Name=NHERF1; Synonyms=NHERF, SLC9A3R1; ORFNames=RCJMB04_3g21;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
CC -!- FUNCTION: Scaffold protein that connects plasma membrane proteins with
CC members of the ezrin/moesin/radixin family and thereby helps to link
CC them to the actin cytoskeleton and to regulate their surface
CC expression. Was first known to play a role in the regulation of the
CC activity and subcellular location of SLC9A3. May enhance Wnt signaling
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endomembrane system; Peripheral membrane protein.
CC Cell projection, filopodium. Cell projection, ruffle. Cell projection,
CC microvillus {ECO:0000250}.
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DR EMBL; AJ719570; CAG31229.1; -; mRNA.
DR RefSeq; NP_001006424.1; NM_001006424.1.
DR AlphaFoldDB; Q5ZM14; -.
DR SMR; Q5ZM14; -.
DR STRING; 9031.ENSGALP00000066224; -.
DR PaxDb; 9031-ENSGALP00000012367; -.
DR GeneID; 422108; -.
DR KEGG; gga:422108; -.
DR CTD; 422108; -.
DR VEuPathDB; HostDB:geneid_422108; -.
DR eggNOG; KOG3528; Eukaryota.
DR InParanoid; Q5ZM14; -.
DR PhylomeDB; Q5ZM14; -.
DR PRO; PR:Q5ZM14; -.
DR Proteomes; UP000000539; Unassembled WGS sequence.
DR GO; GO:0016324; C:apical plasma membrane; IBA:GO_Central.
DR GO; GO:0071944; C:cell periphery; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
DR GO; GO:0030175; C:filopodium; IEA:UniProtKB-SubCell.
DR GO; GO:0005902; C:microvillus; ISS:UniProtKB.
DR GO; GO:0031528; C:microvillus membrane; ISS:UniProtKB.
DR GO; GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
DR GO; GO:0017081; F:chloride channel regulator activity; ISS:UniProtKB.
DR GO; GO:0043495; F:protein-membrane adaptor activity; IBA:GO_Central.
DR GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR GO; GO:0032782; P:bile acid secretion; ISS:UniProtKB.
DR GO; GO:0034635; P:glutathione transport; ISS:UniProtKB.
DR GO; GO:2000146; P:negative regulation of cell motility; ISS:UniProtKB.
DR GO; GO:0051898; P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; ISS:UniProtKB.
DR GO; GO:0010642; P:negative regulation of platelet-derived growth factor receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR GO; GO:0045859; P:regulation of protein kinase activity; ISS:UniProtKB.
DR GO; GO:0070293; P:renal absorption; ISS:UniProtKB.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR CDD; cd00992; PDZ_signaling; 2.
DR Gene3D; 2.30.42.10; -; 2.
DR InterPro; IPR015098; EBP50_C.
DR InterPro; IPR017300; NHERF-1/NHERF-2.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR PANTHER; PTHR14191:SF7; NA(+)_H(+) EXCHANGE REGULATORY COFACTOR NHE-RF1; 1.
DR PANTHER; PTHR14191; PDZ DOMAIN CONTAINING PROTEIN; 1.
DR Pfam; PF09007; EBP50_C; 1.
DR Pfam; PF00595; PDZ; 2.
DR PIRSF; PIRSF037866; EBP50; 1.
DR SMART; SM00228; PDZ; 2.
DR SUPFAM; SSF50156; PDZ domain-like; 2.
DR PROSITE; PS50106; PDZ; 2.
PE 2: Evidence at transcript level;
KW Cell projection; Membrane; Reference proteome; Repeat;
KW Wnt signaling pathway.
FT CHAIN 1..333
FT /note="Na(+)/H(+) exchange regulatory cofactor NHE-RF1"
FT /id="PRO_0000301266"
FT DOMAIN 13..93
FT /note="PDZ 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 135..215
FT /note="PDZ 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT REGION 90..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 232..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..137
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 303..323
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 333 AA; 35805 MW; 352D02280D6AEE65 CRC64;
MSSAPPGPAA PRLCCMEKGP DGYGFHLHGE KGKPGQYIRL VEAGSPAERS GLRAGDRLLE
VDGTNVERES HQQVVERIRA AAGAVRLLVV QPQPEEQPPK THSDPDGEAQ REPPAAETPA
AERSGPEERE LRPRLCRIKK GPNGYGFNLH SEKSRPGQYV RAVDPDSPAE AAGLRAQDRI
VEVNGTSVEG KQHADVVAAI KAGGDETKLL VVGVLADEFF KKCRVVPSEA HLAGPLPEPM
ANGDVEKENG GEPRLNSVSE RPPSPALATS PEGSETHSEP DTQEGDKRSS APSSLLDLDI
PLAVAKERAH QKRTSKRAPQ MDWSKKNELF SNL
//
