ID SGR_ORYSJ Reviewed; 274 AA.
AC Q652K1; A0A0P0XPI7; A3C0X0;
DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 27-MAR-2024, entry version 108.
DE RecName: Full=Protein STAY-GREEN, chloroplastic;
DE AltName: Full=Protein STAYGREEN;
DE Flags: Precursor;
GN Name=SGR; OrderedLocusNames=Os09g0532000, LOC_Os09g36200;
GN ORFNames=OJ1254_E07.23, P0515E01.8;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF VAL-99, INDUCTION BY
RP 6-BENZYLADENINE, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, AND INTERACTION
RP WITH LHCPII COMPLEX.
RC STRAIN=cv. Hwacheong;
RX PubMed=17513504; DOI=10.1105/tpc.106.044891;
RA Park S.Y., Yu J.W., Park J.S., Li J., Yoo S.C., Lee N.Y., Lee S.K.,
RA Jeong S.W., Seo H.S., Koh H.J., Jeon J.S., Park Y.I., Paek N.C.;
RT "The senescence-induced staygreen protein regulates chlorophyll
RT degradation.";
RL Plant Cell 19:1649-1664(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, MUTAGENESIS OF TYR-84, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP INDUCTION BY DARK-INDUCED SENESCENCE; ABSCISIC ACID AND 6-BENZYLADENINE,
RP AND DEVELOPMENTAL STAGE.
RX PubMed=17714430; DOI=10.1111/j.1365-313x.2007.03221.x;
RA Jiang H., Li M., Liang N., Yan H., Wei Y., Xu X., Liu J., Xu Z., Chen F.,
RA Wu G.;
RT "Molecular cloning and function analysis of the stay green gene in rice.";
RL Plant J. 52:197-209(2007).
RN [8]
RP FUNCTION, MUTAGENESIS OF ASN-194, AND DISRUPTION PHENOTYPE.
RX PubMed=17709752; DOI=10.1073/pnas.0705521104;
RA Sato Y., Morita R., Nishimura M., Yamaguchi H., Kusaba M.;
RT "Mendel's green cotyledon gene encodes a positive regulator of the
RT chlorophyll-degrading pathway.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:14169-14174(2007).
RN [9]
RP DEVELOPMENTAL STAGE, AND INDUCTION BY DARK-INDUCED SENESCENCE.
RX PubMed=23816327; DOI=10.1016/j.jplph.2013.05.016;
RA Rong H., Tang Y., Zhang H., Wu P., Chen Y., Li M., Wu G., Jiang H.;
RT "The Stay-Green Rice like (SGRL) gene regulates chlorophyll degradation in
RT rice.";
RL J. Plant Physiol. 170:1367-1373(2013).
CC -!- FUNCTION: Involved in the disassembling mechanism of the intact light-
CC harvesting complex of photosystem II (LHCII) in the thylakoid
CC membranes. Required to trigger chlorophyll degradation during natural
CC and dark-induced leaf senescence. {ECO:0000269|PubMed:17513504,
CC ECO:0000269|PubMed:17709752, ECO:0000269|PubMed:17714430}.
CC -!- SUBUNIT: Interacts with LHCII complex. {ECO:0000269|PubMed:17513504}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane. Plastid,
CC chloroplast stroma.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, roots and developing seeds.
CC {ECO:0000269|PubMed:17714430}.
CC -!- DEVELOPMENTAL STAGE: Constitutively expressed at low level during leaf
CC development, but up-regulated during senescence, when the leaf color
CC changes from green to yellow. {ECO:0000269|PubMed:17513504,
CC ECO:0000269|PubMed:17714430, ECO:0000269|PubMed:23816327}.
CC -!- INDUCTION: Up-regulated by abscisic acid and dark-induced senescence.
CC Down-regulated by 6-benzyladenine. Circadian-regulation with an
CC increased expression during the day and a peak at noon.
CC {ECO:0000269|PubMed:17513504, ECO:0000269|PubMed:17714430,
CC ECO:0000269|PubMed:23816327}.
CC -!- DISRUPTION PHENOTYPE: Stay-green phenotype during dark-induced
CC senescence. {ECO:0000269|PubMed:17709752}.
CC -!- SIMILARITY: Belongs to the staygreen family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAZ45459.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY850134; AAW82954.1; -; mRNA.
DR EMBL; AP005314; BAD46019.1; -; Genomic_DNA.
DR EMBL; AP005567; BAD46266.1; -; Genomic_DNA.
DR EMBL; AP008215; BAF25672.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT09110.1; -; Genomic_DNA.
DR EMBL; CM000146; EAZ45459.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK105810; BAG97378.1; -; mRNA.
DR RefSeq; XP_015611682.1; XM_015756196.1.
DR AlphaFoldDB; Q652K1; -.
DR SMR; Q652K1; -.
DR BioGRID; 816709; 2.
DR STRING; 39947.Q652K1; -.
DR PaxDb; 39947-Q652K1; -.
DR EnsemblPlants; Os09t0532000-01; Os09t0532000-01; Os09g0532000.
DR GeneID; 4347672; -.
DR Gramene; Os09t0532000-01; Os09t0532000-01; Os09g0532000.
DR KEGG; osa:4347672; -.
DR eggNOG; ENOG502QV01; Eukaryota.
DR HOGENOM; CLU_073517_0_0_1; -.
DR InParanoid; Q652K1; -.
DR OMA; EECSCCF; -.
DR OrthoDB; 639337at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000007752; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR Genevisible; Q652K1; OS.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR InterPro; IPR024438; Staygreen.
DR InterPro; IPR010916; TonB_box_CS.
DR PANTHER; PTHR31750:SF4; LP06106P; 1.
DR PANTHER; PTHR31750; PROTEIN STAY-GREEN 1, CHLOROPLASTIC-RELATED; 1.
DR Pfam; PF12638; Staygreen; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..274
FT /note="Protein STAY-GREEN, chloroplastic"
FT /id="PRO_0000425236"
FT MUTAGEN 84
FT /note="Y->C: Loss of activity and stay-green phenotype."
FT /evidence="ECO:0000269|PubMed:17714430"
FT MUTAGEN 99
FT /note="V->M: No effect on LHCPII binding, but decreased
FT chlorophyll degradation and staygreen phenotype."
FT /evidence="ECO:0000269|PubMed:17513504"
FT MUTAGEN 194
FT /note="N->NIL: Loss of function."
FT /evidence="ECO:0000269|PubMed:17709752"
SQ SEQUENCE 274 AA; 30879 MW; 50C9AF72D57C5DC6 CRC64;
MAAATSTMSL LPPITQQQRW HAADSLVVLA SRCHNSRRRR RCRYVVPRAR LFGPAIFEAS
KLKVLFLGVD EEKHQHPGKL PRTYTLTHSD VTARLTLAVS HTINRAQLQG WYNKLQRDEV
VAEWKKVQGH MSLHVHCHIS GGHVLLDLIA GLRYYIFRKE LPVVLKAFVH GDGNLFSRHP
ELEEATVWVY FHSNLPRFNR VECWGPLRDA GAPPEEDDAV AAAAAEEAAA EQMPAAGEWP
RRCPGQCDCC FPPYSLIPWP HQHDVAAADG QPQQ
//
