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Database: UniProt
Entry: Q65LK0
LinkDB: Q65LK0
Original site: Q65LK0 
ID   ADDB_BACLD              Reviewed;        1166 AA.
AC   Q65LK0; Q62WZ1;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   05-DEC-2018, entry version 94.
DE   RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B {ECO:0000255|HAMAP-Rule:MF_01452};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01452};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01452};
DE   AltName: Full=ATP-dependent helicase/nuclease AddB {ECO:0000255|HAMAP-Rule:MF_01452};
GN   Name=addB {ECO:0000255|HAMAP-Rule:MF_01452};
GN   OrderedLocusNames=BLi01156, BL01351;
OS   Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC
OS   12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=279010;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL
RC   NRS-1264 / Gibson 46;
RX   PubMed=15383718; DOI=10.1159/000079829;
RA   Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H.,
RA   Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R.,
RA   Ehrenreich A., Gottschalk G.;
RT   "The complete genome sequence of Bacillus licheniformis DSM13, an
RT   organism with great industrial potential.";
RL   J. Mol. Microbiol. Biotechnol. 7:204-211(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL
RC   NRS-1264 / Gibson 46;
RX   PubMed=15461803; DOI=10.1186/gb-2004-5-10-r77;
RA   Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J.,
RA   Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G.,
RA   Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L.,
RA   Larsen T.S., Sorokin A., Bolotin A., Lapidus A., Galleron N.,
RA   Ehrlich S.D., Berka R.M.;
RT   "Complete genome sequence of the industrial bacterium Bacillus
RT   licheniformis and comparisons with closely related Bacillus species.";
RL   Genome Biol. 5:R77.1-R77.12(2004).
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA
CC       helicase and an ATP-dependent, dual-direction single-stranded
CC       exonuclease. Recognizes the chi site generating a DNA molecule
CC       suitable for the initiation of homologous recombination. The AddB
CC       nuclease domain is not required for chi fragment generation; this
CC       subunit has 5' -> 3' nuclease activity. {ECO:0000255|HAMAP-
CC       Rule:MF_01452}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01452};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB. {ECO:0000255|HAMAP-
CC       Rule:MF_01452}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddB/RexB type 1
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01452}.
DR   EMBL; CP000002; AAU22717.1; -; Genomic_DNA.
DR   EMBL; AE017333; AAU40064.1; -; Genomic_DNA.
DR   RefSeq; WP_009328899.1; NC_006322.1.
DR   ProteinModelPortal; Q65LK0; -.
DR   SMR; Q65LK0; -.
DR   STRING; 279010.BLi01156; -.
DR   EnsemblBacteria; AAU22717; AAU22717; BL01351.
DR   EnsemblBacteria; AAU40064; AAU40064; BLi01156.
DR   GeneID; 3030124; -.
DR   KEGG; bld:BLi01156; -.
DR   KEGG; bli:BL01351; -.
DR   eggNOG; COG3857; LUCA.
DR   HOGENOM; HOG000285805; -.
DR   KO; K16899; -.
DR   OMA; HFASHGL; -.
DR   BioCyc; BLIC279010:BLI_RS05740-MONOMER; -.
DR   Proteomes; UP000000606; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0008409; F:5'-3' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01452; AddB_type1; 1.
DR   InterPro; IPR014140; DNA_helicase_suAddB.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02773; addB_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; ATP-binding; Complete proteome; DNA damage; DNA repair;
KW   Exonuclease; Hydrolase; Iron; Iron-sulfur; Metal-binding; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN         1   1166       ATP-dependent helicase/deoxyribonuclease
FT                                subunit B.
FT                                /FTId=PRO_0000379162.
FT   DOMAIN        1    285       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01452}.
FT   DOMAIN      279    586       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|HAMAP-Rule:MF_01452}.
FT   NP_BIND       8     15       ATP. {ECO:0000255|HAMAP-Rule:MF_01452}.
FT   METAL       801    801       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_01452}.
FT   METAL      1121   1121       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_01452}.
FT   METAL      1124   1124       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_01452}.
FT   METAL      1130   1130       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_01452}.
SQ   SEQUENCE   1166 AA;  134408 MW;  D234A27685429F28 CRC64;
     MGAVFLSGRS GSGKTTFILN EIREKLRDEP LGKPIIFLVP DQMTFLMEYE LSKTPDLGGT
     IRAQVYSFSR LAWRVLQHTG GMNRPFLTGT GIQMLLRRLI EEHKGEFKVY QNASDKSGFT
     EQVERMLTEF KRHCLPPQSI RDMMEGTGKS EYEDERILSD KLHDLYILYS KLEENLENQY
     VQSEDYLTLL AEQIPYAEEI RNAAVYVDGF HQFTPQEMSV LEQLMVHAEE ITFSLTADKP
     FTANSPNDLH LFRMTGKAYY DLYQKAKELG LDPSEVRLEE TKRHRHHPEL QHLERYFDER
     PAKPYPGQTE SLRIMQASNR RTEIEGIARE IHSLIRQGRF RLRDIAVIAR NVEDYKDTIK
     EVFKDCELPF FIDGKESMQN HPLIELIRST LDIIKGNWRY EAVFRCVKTE LLFPEGQPKE
     RLREQIDQLE NYCIAYGIKG DRWTSKDPFV YRRYASLDED FAKTDKEIET ENMLNELKGW
     IVPPIHRLQK RLKKAETVRE MAEAVYLYLE EADVPMKLEQ ERRLAEEGGR IAESRQHEQV
     WDAVIQLLDE FVEMMGTERI SFALFQQMIE TGLESLKFAL IPPALDQVFI GNMDLSRMYG
     TKCTFLIGVN DGILPARPAD DGVLSDEDRE WLKRNGAQLA ATGREQLLDE NFLIYMTLSS
     PSEKLYVSYP IADSEGKTLL PSTVVKRLNE LFPDSEEKML IHEPEQLDDE AQLEFLVNKG
     IALSHLAGQL GIWTRQYAIS DVWWSTYNFL MNEPDRIFSQ NILSSLFFRN KVENLNRHVS
     RDLYGEHIQG SVSRMETFKA CPFSHFASHG LKLKERQFFK LEAPDIGQLF HSALKLISDR
     LHELKLDWRD LTKAQCETLS SDAVERLAPK LQKEILLSSN RHHYVKQKLQ KIIARVSGIL
     SEHAKASGFA PVGIELGFGG KGPLPPMRFT LKNGCTMELV GRIDRVDKAE SSKGLLLRIV
     DYKSSDKGLD LAEVYYGLAL QMLTYLDLSI THSTDWLGMK ASPAGVLYFH VHDPMIQASV
     PLGLDEIEKE IFKKFKMKGL LLGDQEAVKL MDTTLEQGRS NIISAGLKKD GSLRSDSDVV
     AEEDFHVLRR HIRRTFQQAG EEITDGKVSI EPYKLKDRTP CTYCSYRSFC QFDESLEENE
     YRILKPEKDS VILERLKKED EADGDF
//
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