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Database: UniProt
Entry: Q65N62
LinkDB: Q65N62
Original site: Q65N62 
ID   CSHA_BACLD              Reviewed;         487 AA.
AC   Q65N62; Q62YL1;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   16-OCT-2019, entry version 105.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase CshA {ECO:0000255|HAMAP-Rule:MF_01493};
DE            EC=3.6.4.13 {ECO:0000255|HAMAP-Rule:MF_01493};
GN   Name=cshA {ECO:0000255|HAMAP-Rule:MF_01493};
GN   OrderedLocusNames=BLi00546, BL02197;
OS   Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC
OS   12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=279010;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL
RC   NRS-1264 / Gibson 46;
RX   PubMed=15383718; DOI=10.1159/000079829;
RA   Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H.,
RA   Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R.,
RA   Ehrenreich A., Gottschalk G.;
RT   "The complete genome sequence of Bacillus licheniformis DSM13, an
RT   organism with great industrial potential.";
RL   J. Mol. Microbiol. Biotechnol. 7:204-211(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL
RC   NRS-1264 / Gibson 46;
RX   PubMed=15461803; DOI=10.1186/gb-2004-5-10-r77;
RA   Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J.,
RA   Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G.,
RA   Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L.,
RA   Larsen T.S., Sorokin A., Bolotin A., Lapidus A., Galleron N.,
RA   Ehrlich S.D., Berka R.M.;
RT   "Complete genome sequence of the industrial bacterium Bacillus
RT   licheniformis and comparisons with closely related Bacillus species.";
RL   Genome Biol. 5:R77.1-R77.12(2004).
CC   -!- FUNCTION: DEAD-box RNA helicase possibly involved in RNA
CC       degradation. Unwinds dsRNA in both 5'- and 3'-directions, has RNA-
CC       dependent ATPase activity. {ECO:0000255|HAMAP-Rule:MF_01493}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01493};
CC   -!- SUBUNIT: Oligomerizes, may be a member of the RNA degradosome.
CC       {ECO:0000255|HAMAP-Rule:MF_01493}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01493}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. CshA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01493}.
DR   EMBL; AE017333; AAU39502.1; -; Genomic_DNA.
DR   EMBL; CP000002; AAU22147.1; -; Genomic_DNA.
DR   RefSeq; WP_003179112.1; NC_006322.1.
DR   SMR; Q65N62; -.
DR   STRING; 279010.BL02197; -.
DR   DNASU; 3098967; -.
DR   EnsemblBacteria; AAU22147; AAU22147; BL02197.
DR   EnsemblBacteria; AAU39502; AAU39502; BLi00546.
DR   GeneID; 3030407; -.
DR   KEGG; bld:BLi00546; -.
DR   KEGG; bli:BL02197; -.
DR   eggNOG; ENOG4105C1J; Bacteria.
DR   eggNOG; COG0513; LUCA.
DR   HOGENOM; HOG000268810; -.
DR   KO; K05592; -.
DR   OMA; RNPIRIL; -.
DR   OrthoDB; 626183at2; -.
DR   BioCyc; BLIC279010:BLI_RS02700-MONOMER; -.
DR   Proteomes; UP000000606; Chromosome.
DR   GO; GO:0043590; C:bacterial nucleoid; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR   GO; GO:0003724; F:RNA helicase activity; ISS:UniProtKB.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0010501; P:RNA secondary structure unwinding; IEA:InterPro.
DR   HAMAP; MF_01493; DEAD_helicase_CshA; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR030880; DEAD_helicase_CshA.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome; RNA-binding; Stress response.
FT   CHAIN         1    487       DEAD-box ATP-dependent RNA helicase CshA.
FT                                /FTId=PRO_0000280053.
FT   DOMAIN       34    204       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01493}.
FT   DOMAIN      215    375       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_01493}.
FT   NP_BIND      47     54       ATP. {ECO:0000255|HAMAP-Rule:MF_01493}.
FT   MOTIF         3     31       Q motif.
FT   MOTIF       152    155       DEAD box.
SQ   SEQUENCE   487 AA;  54917 MW;  3B710FAEF8733FFC CRC64;
     MTITFQDFQL SSDLTKAIKR MGFEEATPIQ AQTIPLGLAN KDVIGQAQTG TGKTAAFGIP
     LVEKINPESP NIQAIVIAPT RELAIQVSEE LYKIGQDKRA RVLPIYGGQD IGRQIRALKK
     NPHIIVGTPG RLLDHINRRT MRLQTVNTVV LDEADEMLNM GFIEDIESIL SNVPAEHQTL
     LFSATMPAPI KRIAERFMTN PEHVKVKAKE MTVSNIQQFY LEVHERKKFD TLTRLLDIQS
     PELAIVFGRT KRRVDELTEA LNLRGYTAEG IHGDLTQAKR MVALRKFKQG AIEVLVATDV
     AARGLDISGV THVYNFDVPQ DPESYVHRIG RTGRAGKTGM AMTFITPREK DMLRAIEQTT
     KRKMDRMKAP TLDEAIEGQQ QVTVDRIRTI IEENNLNFYM TAAAELLEDH DSVTVVAAAI
     KMMTKEPDNT PVRLTEEAPL RTKRNKNHHH RSSKRRDGGG YRGKNNRSSY DKKRSSNDRR
     QKKSYNS
//
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