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Database: UniProt
Entry: Q66EN4
LinkDB: Q66EN4
Original site: Q66EN4 
ID   RAPA_YERPS              Reviewed;         968 AA.
AC   Q66EN4;
DT   21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   31-JUL-2019, entry version 85.
DE   RecName: Full=RNA polymerase-associated protein RapA {ECO:0000255|HAMAP-Rule:MF_01821};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_01821};
DE   AltName: Full=ATP-dependent helicase HepA {ECO:0000255|HAMAP-Rule:MF_01821};
GN   Name=rapA {ECO:0000255|HAMAP-Rule:MF_01821}; Synonyms=hepA;
GN   OrderedLocusNames=YPTB0658;
OS   Yersinia pseudotuberculosis serotype I (strain IP32953).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=273123;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IP32953;
RX   PubMed=15358858; DOI=10.1073/pnas.0404012101;
RA   Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O.,
RA   Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L.,
RA   Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C.,
RA   Simonet M., Chenal-Francisque V., Souza B., Dacheux D., Elliott J.M.,
RA   Derbise A., Hauser L.J., Garcia E.;
RT   "Insights into the evolution of Yersinia pestis through whole-genome
RT   comparison with Yersinia pseudotuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004).
CC   -!- FUNCTION: Transcription regulator that activates transcription by
CC       stimulating RNA polymerase (RNAP) recycling in case of stress
CC       conditions such as supercoiled DNA or high salt concentrations.
CC       Probably acts by releasing the RNAP, when it is trapped or
CC       immobilized on tightly supercoiled DNA. Does not activate
CC       transcription on linear DNA. Probably not involved in DNA repair.
CC       {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SUBUNIT: Interacts with the RNAP. Has a higher affinity for the
CC       core RNAP than for the holoenzyme. Its ATPase activity is
CC       stimulated by binding to RNAP. {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. RapA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01821}.
DR   EMBL; BX936398; CAH19898.1; -; Genomic_DNA.
DR   RefSeq; WP_011191719.1; NZ_CP009712.1.
DR   EnsemblBacteria; CAH19898; CAH19898; YPTB0658.
DR   KEGG; ypo:BZ17_1899; -.
DR   KEGG; yps:YPTB0658; -.
DR   PATRIC; fig|273123.14.peg.2018; -.
DR   HOGENOM; HOG000218482; -.
DR   KO; K03580; -.
DR   OMA; MSILERD; -.
DR   Proteomes; UP000001011; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10810; -; 1.
DR   HAMAP; MF_01821; Helicase_RapA; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR023949; Helicase_RapA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022737; RapA_C.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   InterPro; IPR040765; Tudor_1_RapA.
DR   InterPro; IPR040766; Tudor_2_RapA.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF12137; RapA_C; 1.
DR   Pfam; PF00176; SNF2_N; 1.
DR   Pfam; PF18339; Tudor_1_RapA; 1.
DR   Pfam; PF18337; Tudor_RapA; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; Complete proteome; DNA-binding; Helicase;
KW   Hydrolase; Nucleotide-binding; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    968       RNA polymerase-associated protein RapA.
FT                                /FTId=PRO_0000207193.
FT   DOMAIN      164    334       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   DOMAIN      490    644       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   NP_BIND     177    184       ATP. {ECO:0000255|HAMAP-Rule:MF_01821}.
FT   MOTIF       280    283       DEAH box.
SQ   SEQUENCE   968 AA;  110109 MW;  F9EA8B6618871DC4 CRC64;
     MPFTLGQRWI SDTESELGLG TVVAIDVRMI TLLFPATGEN RLYARNDSPI TRVMFNPSDT
     ITHHEGWQLK VEEVTQENGL ITYIGTRLDT EETGVAMREV LLDSKLTFSK PQDRLFAGQI
     DRMDRFALRF RARKYQSEQF RLPWSGLRGI RASLIPHQLH IAYEVGQRHA PRVLLADEVG
     LGKTIEAGMI IHQQLLAGRA ERVLIVVPES LQHQWLVEML RRFNLRFSLF DDSRYSEALL
     DSSNPFDTEQ MVICSLDFVR RNKQRLEQLA DASWDLLVVD EAHHLAWSEE APSREYQVIE
     QLAEHIPGVL LLTATPEQLG QQSHFARLRL LDPDRFHDYE EFVNEQQKYR PIADAVTLLL
     GGERLTDDKL NLLGELIDEQ DIEPLLKAAN SQSEDSEAAR QELVTMLMDR HGTSRVLFRN
     TRNGVKGFPH RVLHQIKLPL PTQYQTAIKV SGIMGAKKTL DARAKDMLYP EQIYQEFEGE
     NATWWNFDPR VEWLLNYLVA NRGEKVLVIC AQAATALQLE QVLREREAIR AAVFHEGLSL
     IERDRAAAYF ASEEDGAQVL LCSEIGSEGR NFQFACQLVM FDLPFNPDLL EQRIGRLDRI
     GQNREIQIMV PYLEDTAQAI LVRWYHEGLD AFEHTCPTGR TIYDSSYQEL ISYLATPSEQ
     EGLDEFIHTC RQQHEGLKLQ LEQGRDRLLE MHSNGGEHGQ ELAQSIAEQD NDINLVSFAL
     NLFDIVGINQ EDRSDNLIVL TPSDHMLVPD FPGLPPDGCT VTFDREQALS REDAQFVSWE
     HPIIRNGLDL ILSGDTGSCA VSLLKNKALP VGTLLAELVY VVEAQAPKHL QLTRFLPPTP
     VRMLMDRNGT NLAAQVEFES FNRQLNAVNR HTSSKLVNAV QQEVHTMLQQ AEALVEAQAQ
     ALIETAKREA DDKLSTELAR LEALKAVNPN IRDDEIEALE HNRKMVLENL NQAGWRLDAI
     RLVVVTHQ
//
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