ID CDKL1_RAT Reviewed; 352 AA.
AC Q66HE7;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 24-JAN-2024, entry version 124.
DE RecName: Full=Cyclin-dependent kinase-like 1 {ECO:0000305};
DE EC=2.7.11.22;
GN Name=Cdkl1 {ECO:0000312|RGD:1305080};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.22;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.22;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- DOMAIN: The [NKR]KIAxRE motif seems to be a cyclin-binding region.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
CC protein kinase family. CDC2/CDKX subfamily. {ECO:0000305}.
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DR EMBL; BC081896; AAH81896.1; -; mRNA.
DR RefSeq; NP_001020292.1; NM_001025121.1.
DR RefSeq; XP_006240233.1; XM_006240171.3.
DR RefSeq; XP_006240234.1; XM_006240172.3.
DR AlphaFoldDB; Q66HE7; -.
DR SMR; Q66HE7; -.
DR STRING; 10116.ENSRNOP00000006374; -.
DR PhosphoSitePlus; Q66HE7; -.
DR PaxDb; 10116-ENSRNOP00000006374; -.
DR Ensembl; ENSRNOT00055015064; ENSRNOP00055012031; ENSRNOG00055008919.
DR GeneID; 314198; -.
DR KEGG; rno:314198; -.
DR UCSC; RGD:1305080; rat.
DR AGR; RGD:1305080; -.
DR CTD; 8814; -.
DR RGD; 1305080; Cdkl1.
DR VEuPathDB; HostDB:ENSRNOG00000038720; -.
DR eggNOG; KOG0593; Eukaryota.
DR HOGENOM; CLU_000288_181_1_1; -.
DR InParanoid; Q66HE7; -.
DR OrthoDB; 244018at2759; -.
DR PhylomeDB; Q66HE7; -.
DR TreeFam; TF101031; -.
DR PRO; PR:Q66HE7; -.
DR Proteomes; UP000002494; Chromosome 6.
DR Bgee; ENSRNOG00000038720; Expressed in adult mammalian kidney and 18 other cell types or tissues.
DR GO; GO:0035869; C:ciliary transition zone; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0007507; P:heart development; IEP:RGD.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:1902017; P:regulation of cilium assembly; ISS:UniProtKB.
DR CDD; cd07847; STKc_CDKL1_4; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR24056; CELL DIVISION PROTEIN KINASE; 1.
DR PANTHER; PTHR24056:SF192; CYCLIN-DEPENDENT KINASE-LIKE 1; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Nucleus;
KW Reference proteome; Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..352
FT /note="Cyclin-dependent kinase-like 1"
FT /id="PRO_0000085812"
FT DOMAIN 4..287
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOTIF 45..51
FT /note="[NKR]KIAxRE"
FT ACT_SITE 126
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 10..18
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 352 AA; 40899 MW; DB9CDF5464E66E24 CRC64;
MEKYEKIGKI GEGSYGVVFK CRNRDTGQIV AIKRFLETED DPVIKKIALR EIRMLKQLKH
PNLVSLLEVF RRKRRLHLVF EYCHHTVLHE LDRYQRGVPE PLVKNITWQT LQAVNFCHKH
NCIHRDVKPE NILITKHSVI KLCDFGFARL LTGPGDYYTD YVATRWYRSP ELLVGDTQYG
PPVDVWAIGC VFAELLSGVP LWPGKSDVDQ LYLIRKTLGD LIPRHQQVFS MNQYFSGVKI
PDPEDMETLE LKFPNISYSA LGFLKGCLHM DPAERLTCEQ LLQHPYFDSI RDVGELARPH
DKPTRKTLRQ SRKHLTGLQH LPQLTSSSVL PALDRKKYHC GTRNFNYHFP NI
//
