ID Q68FN4_DANRE Unreviewed; 390 AA.
AC Q68FN4; A0A8M1P7Y8;
DT 11-OCT-2004, integrated into UniProtKB/TrEMBL.
DT 11-OCT-2004, sequence version 1.
DT 27-MAR-2024, entry version 147.
DE SubName: Full=Protein phosphatase 1B {ECO:0000313|RefSeq:NP_571473.1};
DE EC=3.1.3.16 {ECO:0000313|RefSeq:NP_571473.1};
DE SubName: Full=Protein phosphatase type 2C beta {ECO:0000313|EMBL:AAH79530.1};
DE SubName: Full=Protein phosphatase, Mg2+/Mn2+-dependent, 1Ba {ECO:0000313|Ensembl:ENSDARP00000123249};
GN Name=ppm1ba {ECO:0000313|Ensembl:ENSDARP00000123249,
GN ECO:0000313|RefSeq:NP_571473.1, ECO:0000313|ZFIN:ZDB-GENE-991102-16};
GN Synonyms=pp2cb {ECO:0000313|RefSeq:NP_571473.1}, zgc:92031
GN {ECO:0000313|RefSeq:NP_571473.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAH79530.1};
RN [1] {ECO:0000313|RefSeq:NP_571473.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=11821919; DOI=10.1007/s0023901-0008-y;
RA Stothard P., Hansen D., Pilgrim D.;
RT "Evolution of the PP2C family in Caenorhabditis: rapid divergence of the
RT sex-determining protein FEM-2.";
RL J. Mol. Evol. 54:267-282(2002).
RN [2] {ECO:0000313|EMBL:AAH79530.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Whole {ECO:0000313|EMBL:AAH79530.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|RefSeq:NP_571473.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=19439512;
RA Kassahn K.S., Dang V.T., Wilkins S.J., Perkins A.C., Ragan M.A.;
RT "Evolution of gene function and regulatory control after whole-genome
RT duplication: comparative analyses in vertebrates.";
RL Genome Res. 19:1404-1418(2009).
RN [4] {ECO:0000313|Ensembl:ENSDARP00000123249}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000123249};
RG Ensembl;
RL Submitted (FEB-2012) to UniProtKB.
RN [5] {ECO:0000313|Ensembl:ENSDARP00000123249, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000123249};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [6] {ECO:0000313|RefSeq:NP_571473.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26469318;
RA Elkon R., Milon B., Morrison L., Shah M., Vijayakumar S., Racherla M.,
RA Leitch C.C., Silipino L., Hadi S., Weiss-Gayet M., Barras E., Schmid C.D.,
RA Ait-Lounis A., Barnes A., Song Y., Eisenman D.J., Eliyahu E.,
RA Frolenkov G.I., Strome S.E., Durand B., Zaghloul N.A., Jones S.M.,
RA Reith W., Hertzano R.;
RT "RFX transcription factors are essential for hearing in mice.";
RL Nat. Commun. 6:8549-8549(2015).
RN [7] {ECO:0000313|RefSeq:NP_571473.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26034287;
RA Gomez-Marin C., Tena J.J., Acemel R.D., Lopez-Mayorga M., Naranjo S.,
RA de la Calle-Mustienes E., Maeso I., Beccari L., Aneas I., Vielmas E.,
RA Bovolenta P., Nobrega M.A., Carvajal J., Gomez-Skarmeta J.L.;
RT "Evolutionary comparison reveals that diverging CTCF sites are signatures
RT of ancestral topological associating domains borders.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:7542-7547(2015).
RN [8] {ECO:0000313|RefSeq:NP_571473.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=28252024;
RA Bayes A., Collins M.O., Reig-Viader R., Gou G., Goulding D., Izquierdo A.,
RA Choudhary J.S., Emes R.D., Grant S.G.;
RT "Evolution of complexity in the zebrafish synapse proteome.";
RL Nat. Commun. 8:14613-14613(2017).
RN [9] {ECO:0000313|Ensembl:ENSDARP00000157035}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000157035};
RG Ensembl;
RL Submitted (APR-2018) to UniProtKB.
RN [10] {ECO:0000313|RefSeq:NP_571473.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00001482};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000256|ARBA:ARBA00004514}. Membrane
CC {ECO:0000256|ARBA:ARBA00004635}; Lipid-anchor
CC {ECO:0000256|ARBA:ARBA00004635}.
CC -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000256|ARBA:ARBA00006702,
CC ECO:0000256|RuleBase:RU003465}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CR788247; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC079530; AAH79530.1; -; mRNA.
DR RefSeq; NP_571473.1; NM_131398.1.
DR STRING; 7955.ENSDARP00000123249; -.
DR PaxDb; 7955-ENSDARP00000123249; -.
DR DNASU; 30672; -.
DR Ensembl; ENSDART00000144755.3; ENSDARP00000123249.1; ENSDARG00000001888.8.
DR Ensembl; ENSDART00000190925.1; ENSDARP00000157035.1; ENSDARG00000001888.8.
DR GeneID; 30672; -.
DR KEGG; dre:30672; -.
DR AGR; ZFIN:ZDB-GENE-991102-16; -.
DR CTD; 30672; -.
DR ZFIN; ZDB-GENE-991102-16; ppm1ba.
DR eggNOG; KOG0697; Eukaryota.
DR OMA; QDNRVNG; -.
DR OrthoDB; 11028at2759; -.
DR TreeFam; TF313590; -.
DR Proteomes; UP000000437; Chromosome 13.
DR Bgee; ENSDARG00000001888; Expressed in testis and 27 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR GO; GO:0043124; P:negative regulation of canonical NF-kappaB signal transduction; IBA:GO_Central.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IBA:GO_Central.
DR CDD; cd00143; PP2Cc; 1.
DR Gene3D; 1.10.10.430; Phosphatase 2C, C-terminal domain suprefamily; 1.
DR Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR InterPro; IPR015655; PP2C.
DR InterPro; IPR000222; PP2C_BS.
DR InterPro; IPR012911; PP2C_C.
DR InterPro; IPR036580; PP2C_C_sf.
DR InterPro; IPR036457; PPM-type-like_dom_sf.
DR InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR PANTHER; PTHR47992; PROTEIN PHOSPHATASE; 1.
DR PANTHER; PTHR47992:SF105; PROTEIN PHOSPHATASE 1B; 1.
DR Pfam; PF00481; PP2C; 1.
DR Pfam; PF07830; PP2C_C; 1.
DR SMART; SM00332; PP2Cc; 1.
DR SUPFAM; SSF81606; PP2C-like; 1.
DR SUPFAM; SSF81601; Protein serine/threonine phosphatase 2C, C-terminal domain; 1.
DR PROSITE; PS01032; PPM_1; 1.
DR PROSITE; PS51746; PPM_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU003465};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Myristate {ECO:0000256|ARBA:ARBA00022707};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Protein phosphatase {ECO:0000256|ARBA:ARBA00022912,
KW ECO:0000256|RuleBase:RU003465};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q68FN4};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437}.
FT DOMAIN 23..298
FT /note="PPM-type phosphatase"
FT /evidence="ECO:0000259|PROSITE:PS51746"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 390 AA; 42670 MW; B8B4BF143656D338 CRC64;
MGAFLDKPKT EKHNAHGEGN GLRFGLSSMQ GWRVEMEDAH TAAVGLPHGL DDWSFFGVYD
GHAGSRVANY CSKHLLEHIV AAGSADELRK AGAPAPETPA IEAVKRGIRA GFLRIDEHMR
SFTDLRNGMD RSGSTAVAVL LSPEHLYFIN CGDSRALLCR SGHVCFSTMD HKPCDPREKE
RIQNAGGSVM IQRVNGSLAV SRALGDYDYK CVEGKGPTEQ LVSPEPEVFE IARSDAEDEF
VVLACDGIWD VMTNEDLCAF VRSRLEVTDD LERVCNEVVD TSLHKGSRDN MSIVLVCLPN
APQVSEDAVK RDAELDKYLE SRVEELIEKA GEDGVPELAH VMSSLSQENI PNLPPGGGLA
SKHSVIETVY NRLNPQREED GSGADLEDPW
//
