GenomeNet

Database: UniProt
Entry: Q6BP45
LinkDB: Q6BP45
Original site: Q6BP45 
ID   ROK1_DEBHA              Reviewed;         550 AA.
AC   Q6BP45;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 2.
DT   11-DEC-2019, entry version 92.
DE   RecName: Full=ATP-dependent RNA helicase ROK1;
DE            EC=3.6.4.13;
GN   Name=ROK1; OrderedLocusNames=DEHA2E16632g;
OS   Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 /
OS   IGC 2968) (Yeast) (Torulaspora hansenii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX   NCBI_TaxID=284592;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: ATP-dependent RNA helicase involved in 40S ribosomal subunit
CC       biogenesis. Required for the processing and cleavage of 35S pre-rRNA at
CC       sites A0, A1, and A2, leading to mature 18S rRNA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Interacts with the U3 snoRNA and is associated with the 90S
CC       and 40S pre-ribosomes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX52/ROK1
CC       subfamily. {ECO:0000305}.
DR   EMBL; CR382137; CAG88281.2; -; Genomic_DNA.
DR   RefSeq; XP_460025.2; XM_460025.1.
DR   SMR; Q6BP45; -.
DR   STRING; 4959.XP_460025.2; -.
DR   PRIDE; Q6BP45; -.
DR   EnsemblFungi; CAG88281; CAG88281; DEHA2E16632g.
DR   GeneID; 2902212; -.
DR   KEGG; dha:DEHA2E16632g; -.
DR   HOGENOM; HOG000242486; -.
DR   InParanoid; Q6BP45; -.
DR   KO; K14779; -.
DR   OMA; ICTTPRF; -.
DR   OrthoDB; 400908at2759; -.
DR   Proteomes; UP000000599; Chromosome E.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW   Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT   CHAIN           1..550
FT                   /note="ATP-dependent RNA helicase ROK1"
FT                   /id="PRO_0000232303"
FT   DOMAIN          145..322
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          333..497
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   NP_BIND         158..165
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   MOTIF           114..142
FT                   /note="Q motif"
FT   MOTIF           269..272
FT                   /note="DEAD box"
SQ   SEQUENCE   550 AA;  62130 MW;  F74D6CF8EE12C3FD CRC64;
     MDIFRILSRG ASLKKSKDIT TDYAVPSRKQ FVNEKQKEEN LKSVVDKEVD FFHTKKHTSH
     KETEKKNDEG EDEIEDEVPP LTISSDDDAR KLRKLNKSKV SGEDIPLPIG SFEDLIGRYK
     LDKKLLSNLI DAGFTEPTPI QDEAIPISLE GRDLIACAPT GSGKTLAFLI PLVQTILQSN
     PNVKNYGIRG LIISPTNELA VQIFQELNTM TKGKKMKIGI LSKQLANKIN NKIINSSKYD
     ILVSTPLRLI DSIKNETISL SKVEQLVIDE TDKLFDQGFV EQTDEILSNC TNTKLRKSMF
     SATIPSGVEE MAQSIMKDPI RVIIGHKEAA SNTIEQKLVF TGNEEGKLLA IRQMIQQGEF
     KPPIIIFLQS ITRAKALFHE LLYDRLNVDV IHAERTPKQR DEVIKRFKSG DIWVLITTDV
     IARGVDFKGV NLVINYDVPQ SAQAYVHRIG RTGRGGKAGK AVTFFTKEDD KAVKPIINVM
     KQSGCESGYS GWLEDMHKLS KNEKKDVKKH EIKRKQISTV PKIMKQKRKQ REEMIDASKR
     RKQESQSNTE
//
DBGET integrated database retrieval system