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Database: UniProt
Entry: Q6CRF4
LinkDB: Q6CRF4
Original site: Q6CRF4 
ID   DBP4_KLULA              Reviewed;         770 AA.
AC   Q6CRF4;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   16-OCT-2019, entry version 99.
DE   RecName: Full=ATP-dependent RNA helicase DBP4;
DE            EC=3.6.4.13;
GN   Name=DBP4; OrderedLocusNames=KLLA0D09504g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC
OS   1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 /
RC   WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S.,
RA   Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E.,
RA   Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V.,
RA   Barnay S., Blanchin S., Beckerich J.-M., Beyne E., Bleykasten C.,
RA   Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A.,
RA   Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A.,
RA   Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R.,
RA   Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H.,
RA   Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O.,
RA   Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A.,
RA   Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B.,
RA   Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A.,
RA   Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J.,
RA   Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: ATP-dependent RNA helicase required for ribosome
CC       biogenesis. Involved in the release of U14 snoRNA in pre-ribosomal
CC       complexes. Required for pre-rRNA cleavage at site A2 (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Interacts with the U3 and U14 snoRNAs. Associates with
CC       pre-ribosomal complexes (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX10/DBP4
CC       subfamily. {ECO:0000305}.
DR   EMBL; CR382124; CAH00581.1; -; Genomic_DNA.
DR   RefSeq; XP_453485.1; XM_453485.1.
DR   SMR; Q6CRF4; -.
DR   STRING; 28985.XP_453485.1; -.
DR   EnsemblFungi; CAH00581; CAH00581; KLLA0_D09504g.
DR   GeneID; 2892880; -.
DR   KEGG; kla:KLLA0_D09504g; -.
DR   eggNOG; KOG0342; Eukaryota.
DR   eggNOG; COG0513; LUCA.
DR   HOGENOM; HOG000268801; -.
DR   InParanoid; Q6CRF4; -.
DR   KO; K14776; -.
DR   OMA; KVRTKYD; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0032040; C:small-subunit processome; IEA:EnsemblFungi.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034512; F:box C/D snoRNA binding; IEA:EnsemblFungi.
DR   GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008186; F:RNA-dependent ATPase activity; IEA:EnsemblFungi.
DR   GO; GO:0034511; F:U3 snoRNA binding; IEA:EnsemblFungi.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR025313; DUF4217.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF13959; DUF4217; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM01178; DUF4217; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA processing.
FT   CHAIN         1    770       ATP-dependent RNA helicase DBP4.
FT                                /FTId=PRO_0000232201.
FT   DOMAIN       72    246       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      276    435       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      85     92       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF        41     69       Q motif.
FT   MOTIF       194    197       DEAD box.
SQ   SEQUENCE   770 AA;  87827 MW;  E0022DA405E12264 CRC64;
     MAKKQKHNTQ QRKLIRERDS QKIKDLETKV EEYNPKIAKA NLFQDLPISE QTLKGLKEAA
     FIKLTEIQRE SIPLSLKGHD VLGAAKTGSG KTLAFLIPVI EKLYREKWTD MDGLGALIIS
     PTRELAMQIY EVLSKIGKHT TFSAGLVIGG KDVTFEKERI SRINILIGTP GRILQHMDQA
     VGFNTSNLQL LVLDEADRCL DMGFKRTLDA IINNLPASRQ TLLFSATQSQ SLDDLARLSL
     TDYKQVGTME VLNANSSSAT PESLQQSYIE VPLPDKLDIL FSFIKSHLKS KLIVFLSSSK
     QVHFVYETFR KLQPGISLMH LHGRQKQTAR TETLDKFSRA QHVCLFSTDV VARGIDFPSV
     DWVIQVDCPE DVDTYIHRVG RAARFGKEGK SLIMLTPEEE EGFLKRLKTK NIEPSRLNIK
     QSKKKSIKPQ LQSLLFKDPE LKYLGQKAFI SYVRSIYIQK DKEVFHFDKI PLEQFANSLG
     LPGAPKIKMR GMKSIEKAKE LKNTSRQLLT LAKANDEGDV IKENKPEVRT RYDKMFERKN
     QTVLSEHYLN VTKAQAHDDE DEDFIMVKRQ DHELNEAELP ELLVPTSKRA QKKALSRKAT
     LASNGNPTKV KFDDDGKAHP VYELEDEQDF RQNGNSEKQK EEFLSKEAEL MAKVDVEDKQ
     VAKEKRQEKK RKRLEAMRKE MEADMEDSDY EDEPVAYLGT GNLSDDMQSG ADTEDDSQDE
     RQPRKKSKYA DSSEDEEEEE RTTERDRIIE VEEPQTLEDL ESLTANLIGS
//
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