GenomeNet

Database: UniProt
Entry: Q6CXW0
LinkDB: Q6CXW0
Original site: Q6CXW0 
ID   DBP8_KLULA              Reviewed;         435 AA.
AC   Q6CXW0;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   16-OCT-2019, entry version 96.
DE   RecName: Full=ATP-dependent RNA helicase DBP8;
DE            EC=3.6.4.13;
GN   Name=DBP8; OrderedLocusNames=KLLA0A05203g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC
OS   1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 /
RC   WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S.,
RA   Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E.,
RA   Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V.,
RA   Barnay S., Blanchin S., Beckerich J.-M., Beyne E., Bleykasten C.,
RA   Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A.,
RA   Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A.,
RA   Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R.,
RA   Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H.,
RA   Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O.,
RA   Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A.,
RA   Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B.,
RA   Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A.,
RA   Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J.,
RA   Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: ATP-binding RNA helicase involved in 40S ribosomal
CC       subunit biogenesis and is required for the normal formation of 18S
CC       rRNAs through pre-rRNA processing at A0, A1 and A2 sites. Required
CC       for vegetative growth (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX49/DBP8
CC       subfamily. {ECO:0000305}.
DR   EMBL; CR382121; CAH02817.1; -; Genomic_DNA.
DR   RefSeq; XP_451229.1; XM_451229.1.
DR   SMR; Q6CXW0; -.
DR   STRING; 28985.XP_451229.1; -.
DR   PRIDE; Q6CXW0; -.
DR   EnsemblFungi; CAH02817; CAH02817; KLLA0_A05203g.
DR   GeneID; 2896757; -.
DR   KEGG; kla:KLLA0_A05203g; -.
DR   eggNOG; KOG0340; Eukaryota.
DR   eggNOG; ENOG410XPYC; LUCA.
DR   HOGENOM; HOG000268802; -.
DR   InParanoid; Q6CXW0; -.
DR   KO; K14778; -.
DR   OMA; TEHPIDQ; -.
DR   Proteomes; UP000000598; Chromosome A.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis;
KW   RNA-binding; rRNA processing.
FT   CHAIN         1    435       ATP-dependent RNA helicase DBP8.
FT                                /FTId=PRO_0000232289.
FT   DOMAIN       35    211       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      244    391       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      48     55       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF         4     32       Q motif.
FT   MOTIF       157    160       DEAD box.
SQ   SEQUENCE   435 AA;  48395 MW;  2D355BA04E6448CC CRC64;
     MSNSEFKSLG CSKWLVEALN AMKIVQPTAI QKACIPEILK GRDCIGGANT GSGKTIAFAA
     PMLTKWSEDP QGMFGIVLTP TRELAMQIAE QFTAFGSAMN IRVAIVVGGE SIVQQAIELQ
     KRPHFIIATP GRLAHHVLNS GEDTIGGLKR VKFLVLDEAD ILLTETFSKD LATCVSILPP
     KNKRQNLLFT ATMTDQVKAL SDAPQTEGKP PVFTFEVESV DNVAIPKTLE TTYLLVPEHV
     KESYLYQILT SEKYVKSSCI IFVNRTVTAE ILRRTLKSLD VRVTSLHSQM PQQERTNSVQ
     RFRAQAARVL IATDVASRGL DIPIVELVVN YDIPGNPDTF IHRAGRTARA GRHGESLCFV
     TEKDIQRVEA IEERINKKME EFTDVGDTAV IRKSLTKVTA AKRESLMAMD KEGFGERRKL
     QKRKNESKEK THRRT
//
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