ID BTUD_PECAS Reviewed; 250 AA.
AC Q6D654;
DT 15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 27-MAR-2024, entry version 109.
DE RecName: Full=Vitamin B12 import ATP-binding protein BtuD {ECO:0000255|HAMAP-Rule:MF_01005};
DE EC=7.6.2.8 {ECO:0000255|HAMAP-Rule:MF_01005};
DE AltName: Full=Vitamin B12-transporting ATPase {ECO:0000255|HAMAP-Rule:MF_01005};
GN Name=btuD {ECO:0000255|HAMAP-Rule:MF_01005}; OrderedLocusNames=ECA1834;
OS Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS carotovora subsp. atroseptica).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Pectobacterium.
OX NCBI_TaxID=218491;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCRI 1043 / ATCC BAA-672;
RX PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT subsp. atroseptica and characterization of virulence factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
CC -!- FUNCTION: Part of the ABC transporter complex BtuCDF involved in
CC vitamin B12 import. Responsible for energy coupling to the transport
CC system. {ECO:0000255|HAMAP-Rule:MF_01005}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an R-cob(III)alamin(out) + ATP + H2O = ADP + an R-
CC cob(III)alamin(in) + H(+) + phosphate; Xref=Rhea:RHEA:17873,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:140785, ChEBI:CHEBI:456216;
CC EC=7.6.2.8; Evidence={ECO:0000255|HAMAP-Rule:MF_01005};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (BtuD),
CC two transmembrane proteins (BtuC) and a solute-binding protein (BtuF).
CC {ECO:0000255|HAMAP-Rule:MF_01005}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01005}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01005}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Vitamin B12
CC importer (TC 3.A.1.13.1) family. {ECO:0000255|HAMAP-Rule:MF_01005}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX950851; CAG74737.1; -; Genomic_DNA.
DR AlphaFoldDB; Q6D654; -.
DR SMR; Q6D654; -.
DR STRING; 218491.ECA1834; -.
DR KEGG; eca:ECA1834; -.
DR eggNOG; COG4138; Bacteria.
DR HOGENOM; CLU_000604_1_11_6; -.
DR Proteomes; UP000007966; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd03214; ABC_Iron-Siderophores_B12_Hemin; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01005; BtuD; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR023693; ABC_transptr_BtuD.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR42794; HEMIN IMPORT ATP-BINDING PROTEIN HMUV; 1.
DR PANTHER; PTHR42794:SF1; HEMIN IMPORT ATP-BINDING PROTEIN HMUV; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Translocase; Transport.
FT CHAIN 1..250
FT /note="Vitamin B12 import ATP-binding protein BtuD"
FT /id="PRO_0000091953"
FT DOMAIN 3..233
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01005"
FT BINDING 29..36
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01005"
SQ SEQUENCE 250 AA; 27420 MW; 6DE289355961F5C1 CRC64;
MQLRQASVLP RLSPTNAECR PGELLHIIGP NGAGKSTLLA RAAGLLAGEG EVYLAGTPLS
QYTAADLAVR RAYLAQQQPP LALMPVFQYW QRHQPPLAQE YAVEKVVHFL AERLMLTDKL
ARPLTQLSGG EWQRVRLVAA LLQIWPTINP HARLLLLDEP TNSLDVAQQV ALDSLLSELC
RLGIAVVVCA HDLNHSAHHA DRVWLLSAGV LVAQGETEDV MLPEVLSPVF GVAFQRHVVD
GRNWIITRSA
//
