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Database: UniProt
Entry: Q6GBY5
LinkDB: Q6GBY5
Original site: Q6GBY5 
ID   MFD_STAAS               Reviewed;        1168 AA.
AC   Q6GBY5;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   16-OCT-2019, entry version 105.
DE   RecName: Full=Transcription-repair-coupling factor {ECO:0000255|HAMAP-Rule:MF_00969};
DE            Short=TRCF {ECO:0000255|HAMAP-Rule:MF_00969};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00969};
GN   Name=mfd {ECO:0000255|HAMAP-Rule:MF_00969}; OrderedLocusNames=SAS0460;
OS   Staphylococcus aureus (strain MSSA476).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSSA476;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T.,
RA   Churcher C., Clark L., Corton C., Cronin A., Doggett J., Dowd L.,
RA   Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K.,
RA   James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K.,
RA   Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M.,
RA   Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G.,
RA   Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains:
RT   evidence for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC       polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC       release of RNAP and its truncated transcript from the DNA, and
CC       recruitment of nucleotide excision repair machinery to the damaged
CC       site. {ECO:0000255|HAMAP-Rule:MF_00969}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00969}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC       {ECO:0000255|HAMAP-Rule:MF_00969}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the helicase
CC       family. RecG subfamily. {ECO:0000255|HAMAP-Rule:MF_00969}.
DR   EMBL; BX571857; CAG42235.1; -; Genomic_DNA.
DR   RefSeq; WP_000154236.1; NC_002953.3.
DR   SMR; Q6GBY5; -.
DR   PRIDE; Q6GBY5; -.
DR   KEGG; sas:SAS0460; -.
DR   HOGENOM; HOG000216591; -.
DR   KO; K03723; -.
DR   OMA; KRGQPGD; -.
DR   BioCyc; SAUR282459:G1G3P-505-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.30.840; -; 1.
DR   Gene3D; 3.90.1150.50; -; 1.
DR   HAMAP; MF_00969; TRCF; 1.
DR   InterPro; IPR036101; CarD-like/TRCF_dom_sf.
DR   InterPro; IPR003711; CarD-like/TRCF_domain.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR004576; Mfd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR037235; TRCF-like_C.
DR   InterPro; IPR005118; TRCF_C.
DR   InterPro; IPR041471; UvrB_inter.
DR   Pfam; PF02559; CarD_CdnL_TRCF; 1.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF03461; TRCF; 1.
DR   Pfam; PF17757; UvrB_inter; 1.
DR   SMART; SM01058; CarD_TRCF; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00982; TRCF; 1.
DR   SUPFAM; SSF141259; SSF141259; 1.
DR   SUPFAM; SSF143517; SSF143517; 1.
DR   SUPFAM; SSF52540; SSF52540; 4.
DR   TIGRFAMs; TIGR00580; mfd; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; DNA damage; DNA repair; DNA-binding; Helicase;
KW   Hydrolase; Nucleotide-binding.
FT   CHAIN         1   1168       Transcription-repair-coupling factor.
FT                                /FTId=PRO_0000282670.
FT   DOMAIN      633    794       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00969}.
FT   DOMAIN      808    969       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_00969}.
FT   NP_BIND     646    653       ATP. {ECO:0000255|HAMAP-Rule:MF_00969}.
FT   MOTIF       747    750       DEEQ box.
SQ   SEQUENCE   1168 AA;  134218 MW;  2EDB0443A4B544D3 CRC64;
     MTILTTLIKE DNHFQDLNQV FGQANTLVTG LSPSAKVTMI AEKYAQSNQQ LLLITNNLYQ
     ADKLETDLLQ FIDAEELYKY PVQDIMTEEF STQSPQLMSE RIRTLTALAQ GKKGLFIVPL
     NGLKKWLTPV EMWQNHQMTL RVGEDIDVDQ FLNKLVNMGY KRESVVSHIG EFSLRGGIID
     IFPLIGEPIR IELFDTKIDS IRDFDVETQR SKDNVEEVDI TTASDYIITE EVISHLKEEL
     KTAYENTRPK IDKSVRNDLK ETYESFKLFE STYFDHQILR RLVAFMYETP STIIEYFQKD
     AIIAVDEFNR IKETEESLTV ESDSFISNII ESGNGFIGQS FIKYDDFETL IEGYPVTYFS
     LFATTMPIKL NHIIKFSCKP VQQFYGQYDI MRSEFQRYVN QNYHIVVLVE TETKVERMQA
     MLSEMHIPSI TKLHRSMSSG QAVIIEGSLS EGFELPDMGL VVITERELFK SKQKKQRKRT
     KAISNAEKIK SYQDLNVGDY IVHVHHGVGR YLGVETLEVG QTHRDYIKLQ YKGTDQLFVP
     VDQMDQVQKY VASEDKTPKL NKLGGSEWKK TKAKVQQSVE DIAEELIDLY KEREMAEGYQ
     YGEDTAEQTT FELDFPYELT PDQAKSIDEI KDDMQKSRPM DRLLCGDVGY GKTEVAVRAA
     FKAVMEGKQV AFLVPTTILA QQHYETLIER MQDFPVEIQL MSRFRTPKEI KQTKEGLKTG
     FVDIVVGTHK LLSKDIQYKD LGLLIVDEEQ RFGVRHKERI KTLKHNVDVL TLTATPIPRT
     LHMSMLGVRD LSVIETPPEN RFPVQTYVLE QNMSFIKEAL ERELSRDGQV FYLYNKVQSI
     YEKREQLQML MPDANIAVAH GQMTERDLEE TMLSFINNEY DILVTTTIIE TGVDVPNANT
     LIIEDADRFG LSQLYQLRGR VGRSSRIGYA YFLHPANKVL TETAEDRLQA IKEFTELGSG
     FKIAMRDLNI RGAGNLLGKQ QHGFIDTVGF DLYSQMLEEA VNEKRGIKEP ESEVPEVEVD
     LNLDAYLPTE YIANEQAKIE IYKKLRKTET FDQIIDIKDE LIDRFNDYPV EVARLLDIVE
     IKVHALHSGI TLIKDKGKII DIHLSVKATE NIDGEVLFKA TQPLGRTMKV GVQNNAMTIT
     LTKQNQWLDS LKFLVKCIEE SMRISDEA
//
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