ID PUTP_STAAR Reviewed; 512 AA.
AC Q6GFF5;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 102.
DE RecName: Full=Sodium/proline symporter;
DE AltName: Full=Proline permease;
GN Name=putP; OrderedLocusNames=SAR1994;
OS Staphylococcus aureus (strain MRSA252).
OC Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=282458;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MRSA252;
RX PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT for the rapid evolution of virulence and drug resistance.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC -!- FUNCTION: Catalyzes the sodium-dependent uptake of extracellular L-
CC proline (By similarity). Since most S.aureus strains are L-proline
CC auxotrophs, this transporter may aid the bacterial persistence during
CC an infection of tissues with low proline concentrations (By
CC similarity). {ECO:0000250|UniProtKB:P07117,
CC ECO:0000250|UniProtKB:Q2FWY7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out);
CC Xref=Rhea:RHEA:28967, ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC Evidence={ECO:0000250|UniProtKB:P07117};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; BX571856; CAG40979.1; -; Genomic_DNA.
DR RefSeq; WP_000957023.1; NC_002952.2.
DR AlphaFoldDB; Q6GFF5; -.
DR SMR; Q6GFF5; -.
DR KEGG; sar:SAR1994; -.
DR HOGENOM; CLU_018808_15_2_9; -.
DR Proteomes; UP000000596; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005298; F:proline:sodium symporter activity; IEA:InterPro.
DR GO; GO:0031402; F:sodium ion binding; IEA:InterPro.
DR GO; GO:0015824; P:proline transport; IEA:InterPro.
DR CDD; cd11475; SLC5sbd_PutP; 1.
DR Gene3D; 1.20.1730.10; Sodium/glucose cotransporter; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR011851; Na/Pro_symporter.
DR InterPro; IPR001734; Na/solute_symporter.
DR NCBIfam; TIGR02121; Na_Pro_sym; 1.
DR NCBIfam; TIGR00813; sss; 1.
DR PANTHER; PTHR48086; SODIUM/PROLINE SYMPORTER-RELATED; 1.
DR PANTHER; PTHR48086:SF3; SODIUM_PROLINE SYMPORTER; 1.
DR Pfam; PF00474; SSF; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Cell membrane; Ion transport; Membrane; Sodium;
KW Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..512
FT /note="Sodium/proline symporter"
FT /id="PRO_0000364096"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..430
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 467..487
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 512 AA; 55919 MW; 6D1287B9E11A9236 CRC64;
MLTMGTALSQ QVDANWQTYI MIAVYFLILI VIGFYGYKQA TGNLSEYMLG GRSIGPYITA
LSAGASDMSG WMIMGLPGSV YSTGLSAMWI TIGLTLGAYI NYFVVAPRLR VYTELAGDAI
TLPDFFKNRL NDKNNVLKII SGLIIVVFFT LYTHSGFVSG GKLFESAFGL DYHFGLILVA
FIVIFYTFFG GYLAVSITDF FQGVIMLIAM VMVPIVAMMN LNGWGTFHDV AAMKPTNLNL
FKGLSFIGII SLFSWGLGYF GQPHIIVRFM SIKSHKMLPK ARRLGISWMA VGLLGAVAVG
LTGIAFVPAY HIKLEDPETL FIVMSQVLFH PLVGGFLLAA ILAAIMSTIS SQLLVTSSSL
TEDFYKLVRG EEKAKTHQKE FVMVGRLSVL AVAIVAIAIA WNPNDTILNL VGNAWAGFGA
SFSPLVLFAL YWKGLTRAGA VSGMVSGALV VIVWIAWIKP LAHINEIFGL YEIIPGFIVS
VIVTYVVSKL TKKPGAFVET DLNKVRDIVR EK
//
