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Database: UniProt
Entry: Q6K7B8
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Original site: Q6K7B8 
ID   ITPK4_ORYSJ             Reviewed;         355 AA.
AC   Q6K7B8; Q0E1B2;
DT   04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   16-JAN-2019, entry version 77.
DE   RecName: Full=Inositol-tetrakisphosphate 1-kinase 4 {ECO:0000305};
DE            EC=2.7.1.134 {ECO:0000305};
DE   AltName: Full=Inositol 1,3,4-trisphosphate 5/6-kinase 4 {ECO:0000305};
DE            Short=Inositol-triphosphate 5/6-kinase 4 {ECO:0000305};
DE            Short=Ins(1,3,4)P(3) 5/6-kinase 4 {ECO:0000305};
DE            Short=OsITP5/6K-4 {ECO:0000303|PubMed:17961936};
DE            Short=OsITPK4 {ECO:0000303|PubMed:22038091};
DE            EC=2.7.1.159 {ECO:0000305};
GN   Name=ITPK4;
GN   OrderedLocusNames=Os02g0466400 {ECO:0000312|EMBL:BAF08726.1},
GN   LOC_Os02g26720 {ECO:0000305};
GN   ORFNames=OJ1342_D02.13 {ECO:0000312|EMBL:BAD19564.1},
GN   OsJ_06663 {ECO:0000312|EMBL:EAZ22973.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H.,
RA   Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J.,
RA   Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X.,
RA   Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y.,
RA   Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J.,
RA   Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y.,
RA   Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y.,
RA   Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z.,
RA   Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T.,
RA   Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H.,
RA   Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
RA   Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
RA   Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J.,
RA   Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17961936; DOI=10.1016/j.gene.2007.09.006;
RA   Suzuki M., Tanaka K., Kuwano M., Yoshida K.T.;
RT   "Expression pattern of inositol phosphate-related enzymes in rice
RT   (Oryza sativa L.): implications for the phytic acid biosynthetic
RT   pathway.";
RL   Gene 405:55-64(2007).
RN   [7]
RP   INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=22038091; DOI=10.1007/s11103-011-9830-9;
RA   Du H., Liu L., You L., Yang M., He Y., Li X., Xiong L.;
RT   "Characterization of an inositol 1,3,4-trisphosphate 5/6-kinase gene
RT   that is essential for drought and salt stress responses in rice.";
RL   Plant Mol. Biol. 77:547-563(2011).
CC   -!- FUNCTION: Kinase that can phosphorylate various inositol
CC       polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3 and
CC       participates in phytic acid biosynthesis in developing seeds.
CC       Phytic acid is the primary storage form of phosphorus in cereal
CC       grains and other plant seeds. {ECO:0000250|UniProtKB:Q84Y01}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1D-myo-inositol 3,4,5,6-tetrakisphosphate + ATP = 1D-myo-
CC         inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+);
CC         Xref=Rhea:RHEA:12452, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57539, ChEBI:CHEBI:57733, ChEBI:CHEBI:456216;
CC         EC=2.7.1.134; Evidence={ECO:0000305};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1D-myo-inositol 1,3,4-trisphosphate + ATP = 1D-myo-
CC         inositol 1,3,4,5-tetrakisphosphate + ADP + H(+);
CC         Xref=Rhea:RHEA:13253, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57895, ChEBI:CHEBI:58414, ChEBI:CHEBI:456216;
CC         EC=2.7.1.159; Evidence={ECO:0000305};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1D-myo-inositol 1,3,4-trisphosphate + ATP = 1D-myo-
CC         inositol 1,3,4,6-tetrakisphosphate + ADP + H(+);
CC         Xref=Rhea:RHEA:20940, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57660, ChEBI:CHEBI:58414, ChEBI:CHEBI:456216;
CC         EC=2.7.1.159; Evidence={ECO:0000305};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q13572};
CC       Note=Binds 2 magnesium ions per subunit.
CC       {ECO:0000250|UniProtKB:Q13572};
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q13572}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in embryos and at lower
CC       levels in roots, leaves, flowers and anthers.
CC       {ECO:0000269|PubMed:17961936}.
CC   -!- INDUCTION: By drought stress. {ECO:0000269|PubMed:22038091}.
CC   -!- SIMILARITY: Belongs to the ITPK1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF08726.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
DR   EMBL; AP004850; BAD19564.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF08726.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CM000139; EAZ22973.1; -; Genomic_DNA.
DR   EMBL; AK071209; -; NOT_ANNOTATED_CDS; mRNA.
DR   UniGene; Os.53176; -.
DR   SMR; Q6K7B8; -.
DR   STRING; 39947.LOC_Os02g26720.1; -.
DR   PaxDb; Q6K7B8; -.
DR   eggNOG; ENOG410IHA6; Eukaryota.
DR   eggNOG; ENOG4110KIK; LUCA.
DR   HOGENOM; HOG000220790; -.
DR   InParanoid; Q6K7B8; -.
DR   Proteomes; UP000059680; Chromosome 2.
DR   ExpressionAtlas; Q6K7B8; differential.
DR   Genevisible; Q6K7B8; OS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0047325; F:inositol tetrakisphosphate 1-kinase activity; IBA:GO_Central.
DR   GO; GO:0052726; F:inositol-1,3,4-trisphosphate 5-kinase activity; IBA:GO_Central.
DR   GO; GO:0052725; F:inositol-1,3,4-trisphosphate 6-kinase activity; IBA:GO_Central.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0052746; P:inositol phosphorylation; IBA:GO_Central.
DR   GO; GO:0032957; P:inositol trisphosphate metabolic process; IEA:InterPro.
DR   InterPro; IPR008656; Inositol_tetrakis-P_1-kinase.
DR   InterPro; IPR040464; InsP(3)kin_ATP-grasp.
DR   PANTHER; PTHR14217; PTHR14217; 1.
DR   Pfam; PF05770; Ins134_P3_kin; 1.
DR   PIRSF; PIRSF038186; ITPK; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding;
KW   Nucleotide-binding; Reference proteome; Transferase.
FT   CHAIN         1    355       Inositol-tetrakisphosphate 1-kinase 4.
FT                                /FTId=PRO_0000431875.
FT   DOMAIN      107    318       ATP-grasp. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00409}.
FT   NP_BIND     179    190       ATP. {ECO:0000250|UniProtKB:Q13572}.
FT   METAL       272    272       Magnesium 1.
FT                                {ECO:0000250|UniProtKB:Q13572}.
FT   METAL       289    289       Magnesium 1.
FT                                {ECO:0000250|UniProtKB:Q13572}.
FT   METAL       289    289       Magnesium 2.
FT                                {ECO:0000250|UniProtKB:Q13572}.
FT   METAL       291    291       Magnesium 2.
FT                                {ECO:0000250|UniProtKB:Q13572}.
FT   BINDING      65     65       1D-myo-inositol 1,3,4-trisphosphate.
FT                                {ECO:0000250|UniProtKB:Q9XYQ1}.
FT   BINDING     101    101       ATP. {ECO:0000250|UniProtKB:Q13572}.
FT   BINDING     146    146       ATP. {ECO:0000250|UniProtKB:Q13572}.
FT   BINDING     157    157       1D-myo-inositol 1,3,4-trisphosphate.
FT                                {ECO:0000250|UniProtKB:Q9XYQ1}.
FT   BINDING     190    190       1D-myo-inositol 1,3,4-trisphosphate.
FT                                {ECO:0000250|UniProtKB:Q9XYQ1}.
FT   BINDING     205    205       ATP. {ECO:0000250|UniProtKB:Q13572}.
FT   BINDING     291    291       1D-myo-inositol 1,3,4-trisphosphate.
FT                                {ECO:0000250|UniProtKB:Q9XYQ1}.
FT   CONFLICT     72     72       R -> H (in Ref. 5; AK071209).
FT                                {ECO:0000305}.
SQ   SEQUENCE   355 AA;  38497 MW;  2C2E4E7F30632DC7 CRC64;
     MAPELSSPSS SPRYTVGYAL LPEKVSSVVR PSLVALAADR GVRLVAVDVS RPLAEQGPFD
     LLVHKMYDRG WRAQLEELAA RHPGVTVVVD SPGAIDRLLD RATMLDVVSG LRTPVSVPPQ
     VVVSDAAADA DELLARAALR FPLIAKPLAV DGSAESHDMR LVYRRDGVLP LLRAPLVLQE
     FVNHGGVLFK VYVVGDRATC VRRSSLPDVP ARRLLDLDAE PSVPFANISN QPLPPPDDDG
     GAADDDTPAA GFVDEVARGL RRGLGLHLFN FDMIRERSEE HGDRYFIIDI NYFPGYAKMP
     GYEAALTDFF LEMLRGTRPV PEQLGPGSGL DMEARKLEPG LGIGLRELES GRAQA
//
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